FastQCFastQC Report
Fri 17 Jun 2016
SRR1525797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939
Sequences flagged as poor quality0
Sequence length40
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.36101083032490977No Hit
TTTAGGGGGCTCGTCTTAATCAGCAACATCTTTTGATACA70.36101083032490977No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.25786487880350695No Hit
TTTAGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT40.2062919030428056No Hit
TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT40.2062919030428056No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA40.2062919030428056No Hit
TTTAGGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT40.2062919030428056No Hit
TTTAGGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT40.2062919030428056No Hit
TTTAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.2062919030428056No Hit
TTTAGGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA30.15471892728210418No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15471892728210418No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCCCGCGGCCCGA30.15471892728210418No Hit
TTTAGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.15471892728210418No Hit
TTTAGGGGGGCCGAATAGCCTCTCCCCCCCAGCGGCCGGA30.15471892728210418No Hit
TTTAGGGGGCAACAGCCAGGACAACATCCCAAAAAAAAAA30.15471892728210418No Hit
TTTAGGGGGCTCGTCTTAATCAGCACCATCTTTTGATACA30.15471892728210418No Hit
TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGA30.15471892728210418No Hit
TTTAGGGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT30.15471892728210418No Hit
TTTAGGGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTT30.15471892728210418No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1031459515214028No Hit
TTTAGGGGGTCTCGATTTTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCCGCCCGA20.1031459515214028No Hit
TTTAGGGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGATAACGCAGCGTCGACTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGATGGCAACGCAGCGTCGACTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGCTCGTCTTACTCCGCAACATCTTTTGATACA20.1031459515214028No Hit
TTTAGGGGGGTCACCAGTCGACTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGTCAACGCAGAGTCGATTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT20.1031459515214028No Hit
TTTAGGGGGAGTGGACACGAGAGTCGATTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGAGGTTCGCAGAGTCGACTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA20.1031459515214028No Hit
TTTAGGGGGTACGCAGAGTCGCTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGAGGTCCAGATCGACTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGTTAATCAGCCACATCTTTTGATACAAAAAAA20.1031459515214028No Hit
TTTAGGGGGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGTAGAGATGTGGAGGAATACCGCCGGGATGAG20.1031459515214028No Hit
TTTAGGGGGGTGTTGCTGAGCACTTTCTGGGATGTTGTCC20.1031459515214028No Hit
TTTAGGGGGTACGAACGATTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGAAGCATCAACGCAGAGTCGACTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCCGAATAGCCCCTCCACCCAAGCGGCCGGA20.1031459515214028No Hit
TTTAGGGGTGGTACAACGCCGATCGACTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGCTCAGTCGATTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGATCCAGTGACAGATACCATTAGATTCAAGAG20.1031459515214028No Hit
TTTAGGGGGGATCCAGTGACAGATACCATTAGATTCAAGA20.1031459515214028No Hit
TTTAGGGGGGGGAATTCGCGGAGAAAGAAATGGCTCCCCT20.1031459515214028No Hit
TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGCTCGTCTTACTCAGCAACATCTTTTGATACA20.1031459515214028No Hit
TTTAGGGGGACAGTATCAACGCAGAGTCGACTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCCGAATAGCCTCTCCCCCCAAGCGGCCCGA20.1031459515214028No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAA20.1031459515214028No Hit
TTTAGGGGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGAGATGGTATACGCAGAGTCGACTTTTTCTTT20.1031459515214028No Hit
TTTAGGGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGACGCAGAGTCGACTCTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCCGAATCGCCTCTCCACCCAACCGGCCGGA20.1031459515214028No Hit
TTTAGGGGGAAGCATGGTATCAACGCAGAGTCGACTTTTT20.1031459515214028No Hit
TTTAGGGGGAGTGTAACGCAGTCGTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGATCCAGTGACACATACCATTAGATTCAAGA20.1031459515214028No Hit
TTTAGGGGGTATCAACCAGAGTCGACTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGAATCAACGCCGATCGACTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCT20.1031459515214028No Hit
TTTAGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGTCAACGATTTTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGTCAACGCAGAGTCGTCTTTCTTTACCTTGTG20.1031459515214028No Hit
TTTAGGGGGTACCATCGATTTTTTTTTTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGAGTTCACGCAGAGTCGACTTTTTTTTTTTTT20.1031459515214028No Hit
TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCCGA20.1031459515214028No Hit
TTTAGGGGGCTCGTCTTAATCCGCAACATCTTTTGATACA20.1031459515214028No Hit
TTTAGGGGGTGCTGCTGAGGACCTTTCTGGGATGTTGTCC20.1031459515214028No Hit
TTTAGGGGGGATCCAGTGACAGATACCATTAGATTCCAGA20.1031459515214028No Hit
TTTAGGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAA20.1031459515214028No Hit
TTTAGGGGGGCCGAATAGCCTCTCCACCCAAACCGCCCGA20.1031459515214028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC451.7444108E-934.0000044
GGGGGCT251.3663282E-434.0000045
TAGGGGG1600.031.8750023
AGGGGGA750.031.7333344
TTTAGGG2050.031.5121941
TTAGGGG1850.031.2432442
AGGGGGG303.9940278E-428.3333364
GGGGAAG303.9940278E-428.3333366
GGGGGAT250.006417794627.25
GGGGGAA508.391251E-623.8000015
Produced by FastQC (version 0.11.5)