##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525797_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939 Sequences flagged as poor quality 0 Sequence length 40 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.690046415678186 30.0 29.0 33.0 4.0 36.0 2 24.48375451263538 28.0 21.0 31.0 4.0 35.0 3 16.214543579164516 12.0 10.0 23.0 4.0 34.0 4 19.895822588963384 21.0 11.0 28.0 4.0 33.0 5 21.822588963383186 24.0 14.0 30.0 4.0 35.0 6 22.520887055183085 26.0 16.0 30.0 4.0 34.0 7 23.272305312016503 27.0 18.0 31.0 4.0 35.0 8 21.518824136152656 24.0 13.0 30.0 4.0 34.0 9 22.516761217122227 27.0 16.0 31.0 4.0 35.0 10 16.65136668385766 16.0 7.0 25.0 4.0 31.0 11 22.809179989685404 27.0 11.0 33.0 4.0 36.0 12 23.918514698298093 29.0 13.0 34.0 4.0 38.0 13 24.493037648272306 29.0 14.0 35.0 4.0 38.0 14 24.620938628158846 30.0 13.0 35.0 4.0 37.0 15 22.721505930892214 27.0 10.0 34.0 4.0 36.0 16 20.186694172253738 23.0 4.0 31.0 4.0 35.0 17 21.304796286745745 26.0 4.0 32.0 4.0 35.0 18 20.674058793192366 24.0 4.0 31.0 4.0 35.0 19 18.427539969056216 19.0 4.0 30.0 4.0 34.0 20 20.17431665807117 23.0 4.0 31.0 4.0 35.0 21 17.050541516245488 15.0 4.0 28.0 4.0 34.0 22 17.60391954615781 17.0 4.0 29.0 4.0 34.0 23 17.501805054151625 18.0 4.0 29.0 4.0 34.0 24 17.185662712738527 17.0 4.0 29.0 4.0 33.0 25 17.11397627643115 17.0 4.0 29.0 4.0 33.0 26 15.438370293965962 10.0 4.0 28.0 4.0 35.0 27 15.771015987622485 11.0 4.0 28.0 4.0 35.0 28 15.72717895822589 11.0 4.0 28.0 4.0 35.0 29 15.342960288808664 10.0 4.0 28.0 4.0 34.0 30 14.326972666322847 4.0 4.0 26.0 4.0 33.0 31 15.24033006704487 4.0 4.0 29.0 4.0 35.0 32 14.584321815368746 4.0 4.0 28.0 4.0 34.0 33 14.951521402784941 4.0 4.0 29.0 4.0 34.0 34 14.42496132026818 4.0 4.0 28.0 4.0 34.0 35 14.434244455905105 4.0 4.0 28.0 4.0 34.0 36 15.500257864878803 4.0 4.0 32.0 4.0 35.0 37 15.635379061371841 4.0 4.0 32.0 4.0 35.0 38 15.318205260443527 4.0 4.0 32.0 4.0 35.0 39 14.70448684889118 4.0 4.0 31.0 4.0 35.0 40 14.437854564208354 4.0 4.0 31.0 4.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -19.256367041198505 1 2 -16.68970037453184 1 3 -9.25486891385768 1 4 -14.312172284644195 1 5 -14.829026217228467 1 6 -16.865168539325843 1 7 -17.41985018726592 1 8 -16.45131086142322 1 9 -16.194569288389513 1 10 -10.500561797752809 1 11 -17.485393258426964 1 12 -15.763295880149808 1 13 -18.582397003745314 1 14 -18.91516853932584 1 15 -15.217977528089886 1 16 -13.937827715355809 1 17 -12.959925093632961 1 18 -15.29119850187266 1 19 -13.535580524344564 1 20 -15.383895131086142 1 21 -10.155430711610485 1 22 -9.691947565543071 1 23 -10.681647940074907 1 24 -8.457303370786516 1 25 -9.453558052434452 1 26 -7.513483146067415 1 27 -5.644007490636703 1 28 -5.629962546816479 1 29 -5.414419475655432 1 30 -6.9874531835206 1 31 -4.864044943820225 1 32 -4.276966292134832 1 33 -4.302434456928836 1 34 -3.8106741573033727 1 35 -4.044194756554308 1 36 -4.708801498127343 1 37 -6.505430711610483 1 38 -4.377715355805243 1 39 -3.5191011235955063 1 40 -2.762172284644194 4 1 -24.006367041198505 4 2 -20.43970037453184 4 3 -11.25486891385768 4 4 -16.062172284644195 4 5 -16.579026217228467 4 6 -18.365168539325843 4 7 -19.41985018726592 4 8 -17.70131086142322 4 9 -18.194569288389513 4 10 -11.750561797752809 4 11 -18.485393258426964 4 12 -20.51329588014981 4 13 -20.332397003745314 4 14 -20.41516853932584 4 15 -18.967977528089886 4 16 -16.18782771535581 4 17 -17.45992509363296 4 18 -16.29119850187266 4 19 -14.035580524344564 4 20 -16.883895131086142 4 21 -13.405430711610485 4 22 -14.191947565543071 4 23 -14.181647940074907 4 24 -13.707303370786516 4 25 -13.453558052434452 4 26 -12.513483146067415 4 27 -13.144007490636703 4 28 -13.12996254681648 4 29 -12.414419475655432 4 30 -11.9874531835206 4 31 -12.364044943820225 4 32 -11.776966292134832 4 33 -12.052434456928836 4 34 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-3.294194756554308 6 36 2.541198501872657 6 37 1.994569288389517 6 38 -0.3777153558052433 6 39 1.2308988764044937 6 40 1.987827715355806 7 1 1.993632958801495 7 2 7.810299625468161 7 3 -3.7548689138576794 7 4 2.1878277153558052 7 5 10.170973782771533 7 6 9.384831460674157 7 7 8.580149812734081 7 8 5.54868913857678 7 9 8.305430711610487 7 10 8.999438202247191 7 11 1.5146067415730364 7 12 0.9867041198501916 7 13 2.9176029962546863 7 14 3.0848314606741596 7 15 3.532022471910114 7 16 9.062172284644191 7 17 5.790074906367039 7 18 0.4588014981273396 7 19 -0.03558052434456371 7 20 8.866104868913858 7 21 -3.405430711610485 7 22 0.05805243445692909 7 23 -1.6816479400749067 7 24 0.04269662921348427 7 25 -0.7035580524344525 7 26 3.236516853932585 7 27 -1.394007490636703 7 28 4.870037453183521 7 29 4.335580524344568 7 30 5.2625468164794 7 31 -0.6140449438202253 7 32 -3.0269662921348317 7 33 -8.552434456928836 7 34 -8.310674157303373 7 35 -6.044194756554308 7 36 -10.208801498127343 7 37 -5.505430711610483 7 38 -7.627715355805243 7 39 -6.769101123595506 7 40 -5.512172284644194 9 1 0.9936329588014949 9 2 5.360299625468162 9 3 2.7451310861423206 9 4 3.5378277153558066 9 5 4.620973782771532 9 6 5.834831460674156 9 7 6.180149812734083 9 8 8.698689138576778 9 9 8.005430711610487 9 10 6.8494382022471925 9 11 11.514606741573036 9 12 5.486704119850192 9 13 11.467602996254683 9 14 1.9848314606741582 9 15 12.232022471910117 9 16 5.812172284644191 9 17 8.34007490636704 9 18 10.70880149812734 9 19 -1.0355805243445637 9 20 4.916104868913859 9 21 11.394569288389516 9 22 2.4080524344569305 9 23 5.618352059925094 9 24 6.892696629213486 9 25 8.346441947565548 9 26 2.2865168539325857 9 27 3.855992509363297 9 28 1.4700374531835223 9 29 -0.8144194756554324 9 30 6.812546816479401 9 31 7.835955056179774 9 32 6.023033707865169 9 33 8.747565543071165 9 34 3.839325842696626 9 35 8.405805243445691 9 36 9.141198501872658 9 37 12.144569288389516 9 38 5.872284644194757 9 39 1.180898876404493 9 40 2.6878277153558052 10 1 10.393632958801494 10 2 10.760299625468164 10 3 23.14513108614232 10 4 17.537827715355807 10 5 14.22097378277153 10 6 15.034831460674155 10 7 14.580149812734081 10 8 16.29868913857678 10 9 14.805430711610487 10 10 19.049438202247188 10 11 12.114606741573038 10 12 11.88670411985019 10 13 10.267602996254688 10 14 10.58483146067416 10 15 -1.3679775280898845 10 16 10.812172284644191 10 17 11.340074906367036 10 18 12.308801498127341 10 19 11.364419475655435 10 20 6.116104868913858 10 21 4.994569288389513 10 22 15.208052434456928 10 23 16.218352059925092 10 24 13.292696629213484 10 25 11.746441947565547 10 26 10.486516853932585 10 27 4.2559925093632955 10 28 11.07003745318352 10 29 7.185580524344569 10 30 5.212546816479399 10 31 7.035955056179773 10 32 4.623033707865167 10 33 11.147565543071163 10 34 7.839325842696626 10 35 6.205805243445692 10 36 9.741198501872656 10 37 11.344569288389518 10 38 6.872284644194757 10 39 8.980898876404494 10 40 8.887827715355805 13 1 0.9936329588014949 13 2 -7.439700374531839 13 3 0.7451310861423206 13 4 -14.062172284644195 13 5 -5.245692883895133 13 6 -6.365168539325843 13 7 -5.753183520599251 13 8 -4.70131086142322 13 9 -6.194569288389513 13 10 5.249438202247191 13 11 6.181273408239704 13 12 9.153370786516856 13 13 9.33426966292135 13 14 7.918164794007495 13 15 3.3653558052434462 13 16 13.145505617977527 13 17 8.873408239700371 13 18 10.375468164794007 13 19 9.631086142322104 13 20 10.782771535580526 13 21 12.594569288389515 13 22 6.141385767790261 13 23 12.151685393258425 13 24 13.959363295880152 13 25 13.546441947565548 13 26 0.8198501872659172 13 27 3.522659176029965 13 28 -8.796629213483145 13 29 -2.414419475655432 13 30 -2.9874531835206 13 31 -4.364044943820225 13 32 -8.776966292134832 13 33 -4.052434456928836 13 34 -6.227340823970039 13 35 -5.460861423220974 13 36 -2.1254681647940092 13 37 -1.922097378277149 13 38 -1.7943820224719094 13 39 -0.6857677902621724 13 40 -0.17883895131086014 14 1 10.743632958801495 14 2 14.310299625468161 14 3 23.495131086142322 14 4 18.687827715355805 14 5 18.170973782771533 14 6 14.634831460674157 14 7 14.580149812734081 14 8 16.29868913857678 14 9 15.555430711610487 14 10 19.99943820224719 14 11 7.014606741573036 14 12 6.736704119850192 14 13 9.667602996254686 14 14 9.83483146067416 14 15 10.782022471910114 14 16 4.562172284644191 14 17 14.290074906367039 14 18 12.70880149812734 14 19 15.214419475655436 14 20 13.866104868913858 14 21 13.844569288389515 14 22 12.808052434456929 14 23 11.318352059925093 14 24 6.292696629213484 14 25 -4.9535580524344525 14 26 4.486516853932585 14 27 7.355992509363297 14 28 -4.129962546816479 14 29 0.335580524344568 14 30 -1.7374531835206 14 31 4.385955056179775 14 32 4.973033707865168 14 33 6.197565543071164 14 34 -1.0606741573033727 14 35 3.205805243445692 14 36 6.791198501872657 14 37 2.244569288389517 14 38 2.1222846441947567 14 39 -0.01910112359550631 14 40 0.9878277153558059 15 1 -24.006367041198505 15 2 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-14.967977528089886 103 16 -14.687827715355809 103 17 -16.95992509363296 103 18 -12.79119850187266 103 19 -7.035580524344564 103 20 -8.883895131086142 103 21 0.5945692883895148 103 22 1.308052434456929 103 23 1.3183520599250933 103 24 0.29269662921348427 103 25 2.0464419475655475 103 26 2.986516853932585 103 27 2.355992509363297 103 28 2.370037453183521 103 29 3.085580524344568 103 30 3.0125468164794 103 31 3.1359550561797747 103 32 2.2230337078651683 103 33 2.947565543071164 103 34 -1.5606741573033727 103 35 3.205805243445692 103 36 -0.9588014981273432 103 37 2.744569288389517 103 38 -0.6277153558052433 103 39 0.4808988764044937 103 40 0.9878277153558059 104 1 3.493632958801495 104 2 4.060299625468161 104 3 -2.2548689138576794 104 4 -2.5621722846441948 104 5 5.920973782771533 104 6 3.1348314606741567 104 7 5.580149812734081 104 8 4.79868913857678 104 9 2.3054307116104873 104 10 14.249438202247191 104 11 12.014606741573036 104 12 9.986704119850192 104 13 9.167602996254686 104 14 8.58483146067416 104 15 11.032022471910114 104 16 5.812172284644191 104 17 11.540074906367039 104 18 10.20880149812734 104 19 5.464419475655436 104 20 -6.883895131086142 104 21 0.09456928838951484 104 22 -0.6919475655430709 104 23 3.3183520599250933 104 24 4.292696629213484 104 25 10.546441947565548 104 26 -1.013483146067415 104 27 -0.644007490636703 104 28 10.87003745318352 104 29 9.585580524344568 104 30 7.5125468164794 104 31 15.635955056179775 104 32 18.72303370786517 104 33 18.447565543071164 104 34 16.439325842696626 104 35 17.205805243445692 104 36 21.041198501872657 104 37 20.244569288389517 104 38 20.872284644194757 104 39 21.980898876404495 104 40 16.987827715355806 105 1 3.493632958801495 105 2 0.060299625468161366 105 3 -2.7548689138576794 105 4 2.9378277153558052 105 5 5.920973782771533 105 6 5.634831460674157 105 7 1.0801498127340814 105 8 3.79868913857678 105 9 2.8054307116104873 105 10 -1.750561797752809 105 11 1.5146067415730364 105 12 8.486704119850192 105 13 1.6676029962546863 105 14 8.58483146067416 105 15 9.032022471910114 105 16 -6.687827715355809 105 17 -5.959925093632961 105 18 -6.79119850187266 105 19 -12.035580524344564 105 20 -4.883895131086142 105 21 -10.405430711610485 105 22 -9.191947565543071 105 23 -1.6816479400749067 105 24 -9.707303370786516 105 25 -4.4535580524344525 105 26 -9.513483146067415 105 27 -10.144007490636703 105 28 -2.129962546816479 105 29 -9.914419475655432 105 30 -7.9874531835206 105 31 -11.864044943820225 105 32 -6.276966292134832 105 33 -5.052434456928836 105 34 -5.060674157303373 105 35 -0.29419475655430816 105 36 3.041198501872657 105 37 3.244569288389517 105 38 3.3722846441947567 105 39 4.480898876404494 105 40 4.987827715355806 106 1 -0.0063670411985050634 106 2 3.5602996254681614 106 3 -3.2548689138576794 106 4 7.937827715355805 106 5 -3.579026217228467 106 6 7.634831460674157 106 7 4.580149812734081 106 8 5.29868913857678 106 9 6.805430711610487 106 10 -0.750561797752809 106 11 2.5146067415730364 106 12 5.486704119850192 106 13 10.667602996254686 106 14 5.58483146067416 106 15 12.032022471910114 106 16 -16.18782771535581 106 17 -17.45992509363296 106 18 -16.29119850187266 106 19 -14.035580524344564 106 20 -16.883895131086142 106 21 -13.405430711610485 106 22 -14.191947565543071 106 23 -14.181647940074907 106 24 -13.707303370786516 106 25 -13.453558052434452 106 26 -12.513483146067415 106 27 -13.144007490636703 106 28 -13.12996254681648 106 29 -12.414419475655432 106 30 -11.9874531835206 106 31 -12.364044943820225 106 32 -11.776966292134832 106 33 -12.052434456928836 106 34 -11.560674157303373 106 35 -11.794194756554308 106 36 -12.458801498127343 106 37 -12.255430711610483 106 38 -12.127715355805243 106 39 -11.019101123595506 106 40 -10.512172284644194 107 1 1.993632958801495 107 2 -3.4397003745318386 107 3 -3.2548689138576794 107 4 -5.062172284644195 107 5 9.420973782771533 107 6 8.634831460674157 107 7 7.580149812734081 107 8 3.29868913857678 107 9 5.805430711610487 107 10 7.249438202247191 107 11 12.514606741573036 107 12 10.486704119850192 107 13 10.667602996254686 107 14 10.58483146067416 107 15 10.032022471910114 107 16 10.812172284644191 107 17 -9.459925093632961 107 18 2.7088014981273396 107 19 -14.035580524344564 107 20 2.116104868913858 107 21 -4.405430711610485 107 22 3.808052434456929 107 23 6.818352059925093 107 24 3.2926966292134843 107 25 -3.4535580524344525 107 26 -6.513483146067415 107 27 -2.144007490636703 107 28 4.870037453183521 107 29 -3.414419475655432 107 30 14.0125468164794 107 31 3.6359550561797747 107 32 -7.776966292134832 107 33 16.947565543071164 107 34 10.439325842696627 107 35 16.205805243445692 107 36 18.541198501872657 107 37 18.744569288389517 107 38 -9.127715355805243 107 39 19.980898876404495 107 40 20.487827715355806 108 1 0.3269662921348271 108 2 -7.1063670411985065 108 3 -5.9215355805243455 108 4 -7.728838951310861 108 5 -4.579026217228467 108 6 -6.698501872659177 108 7 -3.4198501872659186 108 8 -2.367977528089888 108 9 -2.1945692883895127 108 10 -2.083895131086143 108 11 1.8479400749063686 108 12 -2.8466292134831406 108 13 2.334269662921354 108 14 2.2514981273408274 108 15 -6.634644194756554 108 16 -8.854494382022475 108 17 -2.7932584269662932 108 18 -6.624531835205994 108 19 -3.70224719101123 108 20 -10.217228464419476 108 21 -3.738764044943819 108 22 -6.191947565543071 108 23 -7.181647940074907 108 24 -5.04063670411985 108 25 -0.12022471910112031 108 26 1.486516853932585 108 27 -2.144007490636703 108 28 5.870037453183521 108 29 2.9189138576779 108 30 1.3458801498127322 108 31 6.635955056179775 108 32 7.223033707865168 108 33 5.280898876404496 108 34 6.439325842696627 108 35 5.205805243445692 108 36 7.207865168539325 108 37 10.411235955056185 108 38 10.205617977528089 108 39 3.6475655430711615 108 40 4.154494382022474 112 1 3.493632958801495 112 2 -12.939700374531839 112 3 -2.2548689138576794 112 4 9.937827715355805 112 5 3.420973782771533 112 6 8.634831460674157 112 7 5.580149812734081 112 8 -2.20131086142322 112 9 1.8054307116104873 112 10 1.249438202247191 112 11 -1.9853932584269636 112 12 8.986704119850192 112 13 7.667602996254686 112 14 4.58483146067416 112 15 10.032022471910114 112 16 14.312172284644191 112 17 13.040074906367039 112 18 14.20880149812734 112 19 8.464419475655436 112 20 13.116104868913858 112 21 2.094569288389515 112 22 3.308052434456929 112 23 3.3183520599250933 112 24 3.7926966292134843 112 25 4.0464419475655475 112 26 4.986516853932585 112 27 4.355992509363297 112 28 2.370037453183521 112 29 5.085580524344568 112 30 5.5125468164794 112 31 4.635955056179775 112 32 4.723033707865168 112 33 4.447565543071164 112 34 5.439325842696627 112 35 5.205805243445692 112 36 4.541198501872657 112 37 -2.255430711610483 112 38 0.8722846441947567 112 39 0.9808988764044937 112 40 1.987827715355806 114 1 7.993632958801495 114 2 8.560299625468161 114 3 18.745131086142322 114 4 5.937827715355805 114 5 12.420973782771533 114 6 10.634831460674157 114 7 9.580149812734081 114 8 6.29868913857678 114 9 10.805430711610487 114 10 5.249438202247191 114 11 -2.4853932584269636 114 12 5.486704119850192 114 13 -9.332397003745314 114 14 5.58483146067416 114 15 -18.967977528089886 114 16 -16.18782771535581 114 17 -17.45992509363296 114 18 -16.29119850187266 114 19 -14.035580524344564 114 20 -16.883895131086142 114 21 -13.405430711610485 114 22 -14.191947565543071 114 23 -14.181647940074907 114 24 -13.707303370786516 114 25 -13.453558052434452 114 26 -12.513483146067415 114 27 -13.144007490636703 114 28 -13.12996254681648 114 29 -12.414419475655432 114 30 -11.9874531835206 114 31 -12.364044943820225 114 32 -11.776966292134832 114 33 -12.052434456928836 114 34 -11.560674157303373 114 35 -11.794194756554308 114 36 -12.458801498127343 114 37 -12.255430711610483 114 38 -12.127715355805243 114 39 -11.019101123595506 114 40 -10.512172284644194 119 1 7.993632958801495 119 2 11.560299625468161 119 3 20.745131086142322 119 4 7.937827715355805 119 5 12.420973782771533 119 6 7.634831460674157 119 7 9.580149812734081 119 8 11.29868913857678 119 9 -13.194569288389513 119 10 -8.750561797752809 119 11 -1.4853932584269636 119 12 -3.5132958801498084 119 13 -3.3323970037453137 119 14 0.5848314606741596 119 15 2.032022471910114 119 16 11.812172284644191 119 17 7.540074906367039 119 18 11.70880149812734 119 19 13.964419475655436 119 20 8.116104868913858 119 21 8.594569288389515 119 22 13.808052434456929 119 23 16.818352059925093 119 24 8.292696629213484 119 25 17.546441947565548 119 26 18.486516853932585 119 27 17.855992509363297 119 28 14.87003745318352 119 29 18.585580524344568 119 30 19.0125468164794 119 31 -12.364044943820225 119 32 -11.776966292134832 119 33 -12.052434456928836 119 34 -11.560674157303373 119 35 -11.794194756554308 119 36 -12.458801498127343 119 37 -12.255430711610483 119 38 -12.127715355805243 119 39 -11.019101123595506 119 40 -10.512172284644194 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 288.0 5 45.0 6 22.0 7 13.0 8 16.0 9 15.0 10 24.0 11 37.0 12 41.0 13 43.0 14 61.0 15 72.0 16 59.0 17 67.0 18 58.0 19 59.0 20 63.0 21 74.0 22 82.0 23 120.0 24 116.0 25 110.0 26 113.0 27 92.0 28 77.0 29 57.0 30 50.0 31 22.0 32 29.0 33 4.0 34 9.0 35 0.0 36 0.0 37 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.08982963345379 0.8776458440887971 0.5162622612287042 0.5162622612287042 8 96.95719443011862 1.3924703455389376 0.6704486848891181 0.9798865394533264 9 88.79710893133712 7.227671657201859 2.2715539494062984 1.7036654620547238 10 20.06188757091284 43.115007735946364 16.297060340381638 20.526044352759154 11 22.382671480144403 35.53378029912326 21.918514698298093 20.165033522434246 12 37.0293965961836 20.629190304280556 18.25683341928829 24.08457968024755 13 28.365136668385766 24.49716348633316 23.001547189272824 24.136152656008253 14 22.331098504383704 34.14130995358432 22.279525528623 21.248066013408973 15 26.353790613718413 28.261990716864364 23.826714801444044 21.557503867973182 16 21.299638989169676 23.620422898401237 30.840639504899432 24.239298607529655 17 24.858174316658072 28.674574522949975 20.216606498194945 26.250644662197008 18 24.08457968024755 29.448169159360493 20.577617328519857 25.8896338318721 19 15.348837209302326 21.3953488372093 17.9328165374677 45.32299741602067 20 20.113460546673544 25.8380608561114 23.31098504383703 30.73749355337803 21 19.133574007220215 22.485817431665808 27.385250128932437 30.995358432181536 22 20.06188757091284 22.124806601340897 21.763795771015985 36.04951005673027 23 25.02579979360165 26.26418988648091 28.121775025799796 20.588235294117645 24 18.421052631578945 25.541795665634677 26.831785345717236 29.205366357069146 25 21.465428276573785 26.522187822497422 27.76057791537668 24.251805985552117 26 20.3425012973534 23.923196678775298 33.056564608199274 22.677737415672027 27 19.23671995874162 18.824136152656006 34.29602888086642 27.643115007735947 28 14.956162970603405 20.938628158844764 39.762764311500774 24.342444559051057 29 13.068181818181818 18.59504132231405 43.75 24.586776859504134 30 11.116856256463288 19.493278179937953 45.13960703205791 24.250258531540847 31 10.186142709410548 20.78593588417787 51.96483971044468 17.063081695966908 32 10.985043837029398 16.864363073749356 54.30634347601857 17.84424961320268 33 9.649122807017543 15.841073271413828 55.67595459236326 18.833849329205364 34 9.592573491490459 18.25683341928829 57.71015987622485 14.440433212996389 35 11.357769747031492 16.0557563242127 57.666494579246255 14.919979349509552 36 6.814661848218895 17.191533298915846 58.8022715539494 17.191533298915846 37 5.891472868217054 15.968992248062017 60.46511627906976 17.674418604651162 38 7.890665291387313 18.566271273852504 58.844765342960294 14.698298091799897 39 9.96386164171399 17.39803820340733 56.53071760454311 16.10738255033557 40 7.013924703455389 24.08457968024755 57.71015987622485 11.191335740072201 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 2.5 10 4.0 11 7.0 12 10.0 13 10.0 14 11.5 15 13.0 16 15.5 17 18.0 18 18.0 19 26.0 20 34.0 21 41.0 22 48.0 23 48.0 24 49.0 25 50.0 26 51.0 27 52.0 28 52.0 29 65.5 30 79.0 31 95.5 32 112.0 33 112.0 34 118.5 35 125.0 36 137.5 37 150.0 38 150.0 39 175.0 40 200.0 41 190.0 42 180.0 43 180.0 44 166.0 45 152.0 46 130.0 47 108.0 48 108.0 49 104.5 50 101.0 51 103.5 52 106.0 53 106.0 54 95.0 55 84.0 56 81.0 57 78.0 58 78.0 59 65.0 60 52.0 61 46.5 62 41.0 63 41.0 64 40.0 65 39.0 66 39.5 67 40.0 68 40.0 69 39.5 70 39.0 71 26.0 72 13.0 73 13.0 74 10.0 75 7.0 76 5.0 77 3.0 78 3.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.1031459515214028 8 0.0 9 0.1031459515214028 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.2062919030428056 20 0.0 21 0.0 22 0.0 23 0.0515729757607014 24 0.0515729757607014 25 0.0515729757607014 26 0.6188757091284167 27 0.0 28 0.0 29 0.15471892728210418 30 0.25786487880350695 31 0.25786487880350695 32 0.0 33 0.0515729757607014 34 0.0 35 0.1031459515214028 36 0.1031459515214028 37 0.2062919030428056 38 0.0 39 0.1031459515214028 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.27539969056214 #Duplication Level Percentage of deduplicated Percentage of total 1 95.84245076586433 90.35585353274884 2 3.1181619256017505 5.879319236719958 3 0.5470459518599562 1.547189272821042 4 0.3282275711159737 1.2377514182568334 5 0.05470459518599562 0.25786487880350695 6 0.0 0.0 7 0.10940919037199125 0.7220216606498195 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 7 0.36101083032490977 No Hit TTTAGGGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 7 0.36101083032490977 No Hit TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.25786487880350695 No Hit TTTAGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 4 0.2062919030428056 No Hit TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2062919030428056 No Hit TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA 4 0.2062919030428056 No Hit TTTAGGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 4 0.2062919030428056 No Hit TTTAGGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 4 0.2062919030428056 No Hit TTTAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.2062919030428056 No Hit TTTAGGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA 3 0.15471892728210418 No Hit TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.15471892728210418 No Hit TTTAGGGGGGCCGAATAGCCTCTCCACCCCCGCGGCCCGA 3 0.15471892728210418 No Hit TTTAGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.15471892728210418 No Hit TTTAGGGGGGCCGAATAGCCTCTCCCCCCCAGCGGCCGGA 3 0.15471892728210418 No Hit TTTAGGGGGCAACAGCCAGGACAACATCCCAAAAAAAAAA 3 0.15471892728210418 No Hit TTTAGGGGGCTCGTCTTAATCAGCACCATCTTTTGATACA 3 0.15471892728210418 No Hit TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGA 3 0.15471892728210418 No Hit TTTAGGGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 3 0.15471892728210418 No Hit TTTAGGGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTT 3 0.15471892728210418 No Hit TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1031459515214028 No Hit TTTAGGGGGTCTCGATTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCCGCCCGA 2 0.1031459515214028 No Hit TTTAGGGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGATAACGCAGCGTCGACTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGATGGCAACGCAGCGTCGACTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGCTCGTCTTACTCCGCAACATCTTTTGATACA 2 0.1031459515214028 No Hit TTTAGGGGGGTCACCAGTCGACTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGTCAACGCAGAGTCGATTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT 2 0.1031459515214028 No Hit TTTAGGGGGAGTGGACACGAGAGTCGATTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGAGGTTCGCAGAGTCGACTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCCGA 2 0.1031459515214028 No Hit TTTAGGGGGTACGCAGAGTCGCTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGAGGTCCAGATCGACTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGTTAATCAGCCACATCTTTTGATACAAAAAAA 2 0.1031459515214028 No Hit TTTAGGGGGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGTAGAGATGTGGAGGAATACCGCCGGGATGAG 2 0.1031459515214028 No Hit TTTAGGGGGGTGTTGCTGAGCACTTTCTGGGATGTTGTCC 2 0.1031459515214028 No Hit TTTAGGGGGTACGAACGATTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAAGCATCAACGCAGAGTCGACTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATAGCCCCTCCACCCAAGCGGCCGGA 2 0.1031459515214028 No Hit TTTAGGGGTGGTACAACGCCGATCGACTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGCTCAGTCGATTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGATCCAGTGACAGATACCATTAGATTCAAGAG 2 0.1031459515214028 No Hit TTTAGGGGGGATCCAGTGACAGATACCATTAGATTCAAGA 2 0.1031459515214028 No Hit TTTAGGGGGGGGAATTCGCGGAGAAAGAAATGGCTCCCCT 2 0.1031459515214028 No Hit TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGCTCGTCTTACTCAGCAACATCTTTTGATACA 2 0.1031459515214028 No Hit TTTAGGGGGACAGTATCAACGCAGAGTCGACTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATAGCCTCTCCCCCCAAGCGGCCCGA 2 0.1031459515214028 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAA 2 0.1031459515214028 No Hit TTTAGGGGGCGCAGAGTCGACTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAGATGGTATACGCAGAGTCGACTTTTTCTTT 2 0.1031459515214028 No Hit TTTAGGGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGACGCAGAGTCGACTCTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATCGCCTCTCCACCCAACCGGCCGGA 2 0.1031459515214028 No Hit TTTAGGGGGAAGCATGGTATCAACGCAGAGTCGACTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAGTGTAACGCAGTCGTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGATCCAGTGACACATACCATTAGATTCAAGA 2 0.1031459515214028 No Hit TTTAGGGGGTATCAACCAGAGTCGACTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAATCAACGCCGATCGACTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCT 2 0.1031459515214028 No Hit TTTAGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGTCAACGATTTTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGTCAACGCAGAGTCGTCTTTCTTTACCTTGTG 2 0.1031459515214028 No Hit TTTAGGGGGTACCATCGATTTTTTTTTTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGAGTTCACGCAGAGTCGACTTTTTTTTTTTTT 2 0.1031459515214028 No Hit TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCCGA 2 0.1031459515214028 No Hit TTTAGGGGGCTCGTCTTAATCCGCAACATCTTTTGATACA 2 0.1031459515214028 No Hit TTTAGGGGGTGCTGCTGAGGACCTTTCTGGGATGTTGTCC 2 0.1031459515214028 No Hit TTTAGGGGGGATCCAGTGACAGATACCATTAGATTCCAGA 2 0.1031459515214028 No Hit TTTAGGGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAA 2 0.1031459515214028 No Hit TTTAGGGGGGCCGAATAGCCTCTCCACCCAAACCGCCCGA 2 0.1031459515214028 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.05157297576070139 0.0 0.0 0.0 0.0 11 0.05157297576070139 0.0 0.0 0.0 0.0 12 0.05157297576070139 0.0 0.0 0.0 0.0 13 0.15471892728210418 0.0 0.0 0.0 0.0 14 0.15471892728210418 0.0 0.0 0.0 0.0 15 0.15471892728210418 0.0 0.0 0.0 0.0 16 0.15471892728210418 0.0 0.0 0.0 0.0 17 0.15471892728210418 0.0 0.0 0.0 0.0 18 0.15471892728210418 0.0 0.0 0.0 0.0 19 0.15471892728210418 0.0 0.0 0.0 0.0 20 0.15471892728210418 0.0 0.0 0.0 0.0 21 0.15471892728210418 0.0 0.0 0.0 0.0 22 0.15471892728210418 0.0 0.0 0.0 0.0 23 0.15471892728210418 0.0 0.0 0.0 0.0 24 0.15471892728210418 0.0 0.0 0.0 0.0 25 0.15471892728210418 0.0 0.0 0.0 0.0 26 0.15471892728210418 0.0 0.0 0.0 0.0 27 0.15471892728210418 0.0 0.0 0.0 0.0 28 0.15471892728210418 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGC 45 1.7444108E-9 34.000004 4 GGGGGCT 25 1.3663282E-4 34.000004 5 TAGGGGG 160 0.0 31.875002 3 AGGGGGA 75 0.0 31.733334 4 TTTAGGG 205 0.0 31.512194 1 TTAGGGG 185 0.0 31.243244 2 AGGGGGG 30 3.9940278E-4 28.333336 4 GGGGAAG 30 3.9940278E-4 28.333336 6 GGGGGAT 25 0.0064177946 27.2 5 GGGGGAA 50 8.391251E-6 23.800001 5 >>END_MODULE