FastQCFastQC Report
Fri 17 Jun 2016
SRR1525796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1767
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGATTTCTTGTTCTTTACTGTGGGAATTTTGCT50.2829654782116582No Hit
TTTAGGGGGCGCGGAAATGATAGGACAATTTCCAGTATTT20.11318619128466327No Hit
TTTAGGGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC20.11318619128466327No Hit
TTTAGGGGGAATGGACCCTCATTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11318619128466327No Hit
TTTAGGGGGGTGTAACATTGGTGAAGGATCCAGGAATATG20.11318619128466327No Hit
TTTAGGGGGTGACAAAGGCTGAGGTAAGGTTACTAGGCAG20.11318619128466327No Hit
TTTAGGGGGCTGCTGCCCTGGAGATGCCGTCACCTTCCAC20.11318619128466327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC308.064844E-634.04
TTTAGGG1850.032.1621631
TTAGGGG1750.032.057142
TAGGGGG1600.031.8753
AGGGGGG601.2732926E-1131.1666664
AGGGGGA552.3464963E-1030.909094
GGGGGGA352.3241171E-529.1428575
GGGGGAT250.006270388627.1999995
Produced by FastQC (version 0.11.5)