##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525796_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767 Sequences flagged as poor quality 0 Sequence length 40 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.503112620260328 30.0 4.0 35.0 4.0 36.0 2 20.530277306168646 26.0 4.0 33.0 4.0 36.0 3 18.050367855121674 12.0 4.0 34.0 4.0 37.0 4 16.530843237125072 13.0 4.0 30.0 4.0 35.0 5 17.079230333899265 16.0 4.0 30.0 4.0 35.0 6 15.694963214487833 10.0 4.0 28.0 4.0 35.0 7 17.243350311262027 11.0 4.0 32.0 4.0 36.0 8 17.350311262026032 4.0 4.0 32.0 4.0 36.0 9 16.50877192982456 4.0 4.0 31.0 4.0 36.0 10 11.93152235427278 4.0 4.0 20.0 4.0 32.0 11 11.164685908319186 4.0 4.0 17.0 4.0 31.0 12 11.297113752122241 4.0 4.0 19.0 4.0 32.0 13 11.41482739105829 4.0 4.0 19.0 4.0 32.0 14 11.031126202603282 4.0 4.0 18.0 4.0 32.0 15 10.752122241086587 4.0 4.0 16.0 4.0 33.0 16 8.202037351443124 4.0 4.0 4.0 4.0 23.0 17 7.807583474816073 4.0 4.0 4.0 4.0 20.0 18 7.538766270514997 4.0 4.0 4.0 4.0 20.0 19 7.77645727221279 4.0 4.0 4.0 4.0 22.0 20 7.78494623655914 4.0 4.0 4.0 4.0 22.0 21 7.875495189586871 4.0 4.0 4.0 4.0 24.0 22 7.637238256932654 4.0 4.0 4.0 4.0 22.0 23 7.718166383701188 4.0 4.0 4.0 4.0 22.0 24 7.584606677985286 4.0 4.0 4.0 4.0 21.0 25 7.621958121109225 4.0 4.0 4.0 4.0 22.0 26 7.932088285229202 4.0 4.0 4.0 4.0 26.0 27 8.043010752688172 4.0 4.0 4.0 4.0 27.0 28 7.971703452178835 4.0 4.0 4.0 4.0 27.0 29 7.89417091114884 4.0 4.0 4.0 4.0 25.0 30 7.912846632710809 4.0 4.0 4.0 4.0 27.0 31 7.745897000565931 4.0 4.0 4.0 4.0 29.0 32 7.5529145444255805 4.0 4.0 4.0 4.0 25.0 33 7.731182795698925 4.0 4.0 4.0 4.0 27.0 34 7.707979626485569 4.0 4.0 4.0 4.0 27.0 35 7.624221844934918 4.0 4.0 4.0 4.0 27.0 36 7.345217883418223 4.0 4.0 4.0 4.0 28.0 37 7.099603848330504 4.0 4.0 4.0 4.0 23.0 38 7.256932654216186 4.0 4.0 4.0 4.0 26.0 39 7.206564799094511 4.0 4.0 4.0 4.0 26.0 40 7.168081494057725 4.0 4.0 4.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -15.512820512820515 1 2 -15.077545787545787 1 3 -13.35611721611722 1 4 -10.525347985347985 1 5 -12.458021978021975 1 6 -9.227179487179486 1 7 -10.184139194139194 1 8 -10.218168498168497 1 9 -9.51747252747253 1 10 -5.98813186813187 1 11 -5.470512820512818 1 12 -5.641684981684978 1 13 -5.382307692307695 1 14 -5.603150183150182 1 15 -5.310842490842491 1 16 -3.3318681318681316 1 17 -2.905677655677656 1 18 -2.677545787545787 1 19 -2.955677655677656 1 20 -2.6237728937728946 1 21 -2.555824175824176 1 22 -2.2691208791208783 1 23 -2.2825274725274722 1 24 -2.07084249084249 1 25 -2.107728937728937 1 26 -2.178571428571428 1 27 -2.426630036630037 1 28 -2.358205128205129 1 29 -2.5439926739926753 1 30 -2.44054945054945 1 31 -2.7452014652014647 1 32 -2.5663369963369966 1 33 -2.4700366300366294 1 34 -2.4507692307692306 1 35 -2.5082051282051268 1 36 -2.1573626373626382 1 37 -2.071721611721613 1 38 -2.218388278388278 1 39 -2.1063369963369976 1 40 -2.163516483516484 2 1 7.687179487179485 2 2 3.9224542124542126 2 3 13.64388278388278 2 4 -10.525347985347985 2 5 -12.458021978021975 2 6 -9.227179487179486 2 7 -10.184139194139194 2 8 -10.218168498168497 2 9 -9.51747252747253 2 10 -5.98813186813187 2 11 -5.470512820512818 2 12 -5.641684981684978 2 13 -5.382307692307695 2 14 -5.603150183150182 2 15 -5.310842490842491 2 16 -3.3318681318681316 2 17 -2.905677655677656 2 18 -2.677545787545787 2 19 -2.955677655677656 2 20 -2.6237728937728946 2 21 -2.555824175824176 2 22 -2.2691208791208783 2 23 -2.2825274725274722 2 24 -2.07084249084249 2 25 -2.107728937728937 2 26 -2.178571428571428 2 27 -2.426630036630037 2 28 -2.358205128205129 2 29 -2.5439926739926753 2 30 -2.44054945054945 2 31 -2.7452014652014647 2 32 -2.5663369963369966 2 33 -2.4700366300366294 2 34 -2.4507692307692306 2 35 -2.5082051282051268 2 36 -2.1573626373626382 2 37 -2.071721611721613 2 38 -2.218388278388278 2 39 -2.1063369963369976 2 40 -2.163516483516484 3 1 7.687179487179485 3 2 10.922454212454213 3 3 -13.35611721611722 3 4 -10.525347985347985 3 5 -12.458021978021975 3 6 -9.227179487179486 3 7 -10.184139194139194 3 8 -10.218168498168497 3 9 -9.51747252747253 3 10 -5.98813186813187 3 11 -5.470512820512818 3 12 -5.641684981684978 3 13 -5.382307692307695 3 14 -5.603150183150182 3 15 -5.310842490842491 3 16 -3.3318681318681316 3 17 -2.905677655677656 3 18 -2.677545787545787 3 19 -2.955677655677656 3 20 -2.6237728937728946 3 21 -2.555824175824176 3 22 -2.2691208791208783 3 23 -2.2825274725274722 3 24 -2.07084249084249 3 25 -2.107728937728937 3 26 -2.178571428571428 3 27 -2.426630036630037 3 28 -2.358205128205129 3 29 -2.5439926739926753 3 30 -2.44054945054945 3 31 -2.7452014652014647 3 32 -2.5663369963369966 3 33 -2.4700366300366294 3 34 -2.4507692307692306 3 35 -2.5082051282051268 3 36 -2.1573626373626382 3 37 -2.071721611721613 3 38 -2.218388278388278 3 39 -2.1063369963369976 3 40 -2.163516483516484 5 1 -18.312820512820515 5 2 -15.077545787545787 5 3 -13.35611721611722 5 4 -10.525347985347985 5 5 -12.458021978021975 5 6 -9.227179487179486 5 7 -10.184139194139194 5 8 -10.218168498168497 5 9 -9.51747252747253 5 10 -5.98813186813187 5 11 -5.470512820512818 5 12 -5.641684981684978 5 13 -5.382307692307695 5 14 -5.603150183150182 5 15 -5.310842490842491 5 16 -3.3318681318681316 5 17 -2.905677655677656 5 18 -2.677545787545787 5 19 -2.955677655677656 5 20 -2.6237728937728946 5 21 -2.555824175824176 5 22 -2.2691208791208783 5 23 -2.2825274725274722 5 24 -2.07084249084249 5 25 -2.107728937728937 5 26 -2.178571428571428 5 27 -2.426630036630037 5 28 -2.358205128205129 5 29 -2.5439926739926753 5 30 -2.44054945054945 5 31 -2.7452014652014647 5 32 -2.5663369963369966 5 33 -2.4700366300366294 5 34 -2.4507692307692306 5 35 -2.5082051282051268 5 36 -2.1573626373626382 5 37 -2.071721611721613 5 38 -2.218388278388278 5 39 -2.1063369963369976 5 40 -2.163516483516484 10 1 11.937179487179485 10 2 7.4224542124542126 10 3 9.14388278388278 10 4 4.9746520146520155 10 5 7.791978021978025 10 6 11.022820512820514 10 7 12.815860805860806 10 8 11.781831501831503 10 9 13.48252747252747 10 10 -0.9881318681318696 10 11 1.529487179487182 10 12 1.3583150183150217 10 13 2.6176923076923053 10 14 1.3968498168498176 10 15 0.9391575091575088 10 16 2.9181318681318684 10 17 3.594322344322344 10 18 3.822454212454213 10 19 3.294322344322344 10 20 3.8762271062271054 10 21 -2.555824175824176 10 22 -2.2691208791208783 10 23 -2.2825274725274722 10 24 -2.07084249084249 10 25 -2.107728937728937 10 26 -2.178571428571428 10 27 -2.426630036630037 10 28 -2.358205128205129 10 29 -2.5439926739926753 10 30 -2.44054945054945 10 31 -2.7452014652014647 10 32 -2.5663369963369966 10 33 -2.4700366300366294 10 34 -2.4507692307692306 10 35 -2.5082051282051268 10 36 -2.1573626373626382 10 37 -2.071721611721613 10 38 -2.218388278388278 10 39 -2.1063369963369976 10 40 -2.163516483516484 11 1 14.687179487179485 11 2 16.922454212454213 11 3 19.64388278388278 11 4 21.474652014652015 11 5 17.541978021978025 11 6 7.772820512820514 11 7 17.815860805860808 11 8 19.781831501831505 11 9 16.482527472527472 11 10 20.01186813186813 11 11 -5.470512820512818 11 12 -5.641684981684978 11 13 -5.382307692307695 11 14 -5.603150183150182 11 15 -5.310842490842491 11 16 -3.3318681318681316 11 17 -2.905677655677656 11 18 -2.677545787545787 11 19 -2.955677655677656 11 20 -2.6237728937728946 11 21 -2.555824175824176 11 22 -2.2691208791208783 11 23 -2.2825274725274722 11 24 -2.07084249084249 11 25 -2.107728937728937 11 26 -2.178571428571428 11 27 -2.426630036630037 11 28 -2.358205128205129 11 29 -2.5439926739926753 11 30 -2.44054945054945 11 31 -2.7452014652014647 11 32 -2.5663369963369966 11 33 -2.4700366300366294 11 34 -2.4507692307692306 11 35 -2.5082051282051268 11 36 -2.1573626373626382 11 37 -2.071721611721613 11 38 -2.218388278388278 11 39 -2.1063369963369976 11 40 -2.163516483516484 14 1 14.687179487179485 14 2 13.922454212454213 14 3 19.64388278388278 14 4 22.474652014652015 14 5 16.541978021978025 14 6 -9.227179487179486 14 7 -10.184139194139194 14 8 -10.218168498168497 14 9 -9.51747252747253 14 10 -5.98813186813187 14 11 -5.470512820512818 14 12 -5.641684981684978 14 13 -5.382307692307695 14 14 -5.603150183150182 14 15 -5.310842490842491 14 16 -3.3318681318681316 14 17 -2.905677655677656 14 18 -2.677545787545787 14 19 -2.955677655677656 14 20 -2.6237728937728946 14 21 -2.555824175824176 14 22 -2.2691208791208783 14 23 -2.2825274725274722 14 24 -2.07084249084249 14 25 -2.107728937728937 14 26 -2.178571428571428 14 27 -2.426630036630037 14 28 -2.358205128205129 14 29 -2.5439926739926753 14 30 -2.44054945054945 14 31 -2.7452014652014647 14 32 -2.5663369963369966 14 33 -2.4700366300366294 14 34 -2.4507692307692306 14 35 -2.5082051282051268 14 36 -2.1573626373626382 14 37 -2.071721611721613 14 38 -2.218388278388278 14 39 -2.1063369963369976 14 40 -2.163516483516484 16 1 12.687179487179485 16 2 13.922454212454213 16 3 15.64388278388278 16 4 10.474652014652015 16 5 18.541978021978025 16 6 -9.227179487179486 16 7 -10.184139194139194 16 8 -10.218168498168497 16 9 -9.51747252747253 16 10 -5.98813186813187 16 11 -5.470512820512818 16 12 -5.641684981684978 16 13 -5.382307692307695 16 14 -5.603150183150182 16 15 -5.310842490842491 16 16 -3.3318681318681316 16 17 -2.905677655677656 16 18 -2.677545787545787 16 19 -2.955677655677656 16 20 -2.6237728937728946 16 21 -2.555824175824176 16 22 -2.2691208791208783 16 23 -2.2825274725274722 16 24 -2.07084249084249 16 25 -2.107728937728937 16 26 -2.178571428571428 16 27 -2.426630036630037 16 28 -2.358205128205129 16 29 -2.5439926739926753 16 30 -2.44054945054945 16 31 -2.7452014652014647 16 32 -2.5663369963369966 16 33 -2.4700366300366294 16 34 -2.4507692307692306 16 35 -2.5082051282051268 16 36 -2.1573626373626382 16 37 -2.071721611721613 16 38 -2.218388278388278 16 39 -2.1063369963369976 16 40 -2.163516483516484 17 1 -3.1461538461538474 17 2 -0.4108791208791196 17 3 -8.35611721611722 17 4 -0.19201465201465062 17 5 0.20864468864469288 17 6 0.4394871794871804 17 7 3.3158608058608063 17 8 3.115164835164835 17 9 4.1491941391941385 17 10 -2.9881318681318696 17 11 4.029487179487182 17 12 2.5249816849816877 17 13 1.1176923076923053 17 14 6.73018315018315 17 15 1.1891575091575088 17 16 8.001465201465201 17 17 6.927655677655678 17 18 7.489120879120879 17 19 6.377655677655678 17 20 5.0428937728937715 17 21 8.777509157509158 17 22 9.230879120879122 17 23 9.550805860805863 17 24 9.262490842490845 17 25 8.725604395604396 17 26 5.154761904761906 17 27 4.906703296703297 17 28 4.975128205128205 17 29 5.456007326007325 17 30 3.05945054945055 17 31 6.254798534798535 17 32 5.433663003663003 17 33 6.529963369963371 17 34 6.549230769230769 17 35 6.491794871794873 17 36 5.342637362637362 17 37 4.261611721611721 17 38 4.448278388278388 17 39 6.0603296703296685 17 40 5.16981684981685 18 1 -18.312820512820515 18 2 -15.077545787545787 18 3 -13.35611721611722 18 4 -10.525347985347985 18 5 -12.458021978021975 18 6 -9.227179487179486 18 7 -10.184139194139194 18 8 -10.218168498168497 18 9 -9.51747252747253 18 10 -5.98813186813187 18 11 -5.470512820512818 18 12 -5.641684981684978 18 13 -5.382307692307695 18 14 -5.603150183150182 18 15 -5.310842490842491 18 16 -3.3318681318681316 18 17 -2.905677655677656 18 18 -2.677545787545787 18 19 -2.955677655677656 18 20 -2.6237728937728946 18 21 -2.555824175824176 18 22 -2.2691208791208783 18 23 -2.2825274725274722 18 24 -2.07084249084249 18 25 -2.107728937728937 18 26 -2.178571428571428 18 27 -2.426630036630037 18 28 -2.358205128205129 18 29 -2.5439926739926753 18 30 -2.44054945054945 18 31 -2.7452014652014647 18 32 -2.5663369963369966 18 33 -2.4700366300366294 18 34 -2.4507692307692306 18 35 -2.5082051282051268 18 36 -2.1573626373626382 18 37 -2.071721611721613 18 38 -2.218388278388278 18 39 -2.1063369963369976 18 40 -2.163516483516484 19 1 -11.312820512820515 19 2 -6.7442124542124535 19 3 -10.689450549450552 19 4 -8.858681318681317 19 5 -6.458021978021975 19 6 -4.56051282051282 19 7 -3.5174725274725276 19 8 -4.884835164835163 19 9 -3.1841391941391954 19 10 -1.3214652014652035 19 11 2.862820512820516 19 12 4.691648351648356 19 13 4.951025641025639 19 14 2.0635164835164836 19 15 5.022490842490843 19 16 4.3347985347985345 19 17 5.427655677655678 19 18 6.989120879120879 19 19 -2.955677655677656 19 20 -2.6237728937728946 19 21 -2.555824175824176 19 22 -2.2691208791208783 19 23 -2.2825274725274722 19 24 -2.07084249084249 19 25 -2.107728937728937 19 26 -2.178571428571428 19 27 -2.426630036630037 19 28 -2.358205128205129 19 29 -2.5439926739926753 19 30 -2.44054945054945 19 31 -2.7452014652014647 19 32 -2.5663369963369966 19 33 -2.4700366300366294 19 34 -2.4507692307692306 19 35 -2.5082051282051268 19 36 -2.1573626373626382 19 37 -2.071721611721613 19 38 -2.218388278388278 19 39 -2.1063369963369976 19 40 -2.163516483516484 22 1 -18.312820512820515 22 2 -15.077545787545787 22 3 -13.35611721611722 22 4 -10.525347985347985 22 5 -12.458021978021975 22 6 -9.227179487179486 22 7 -10.184139194139194 22 8 -10.218168498168497 22 9 -9.51747252747253 22 10 -5.98813186813187 22 11 -5.470512820512818 22 12 -5.641684981684978 22 13 -5.382307692307695 22 14 -5.603150183150182 22 15 -5.310842490842491 22 16 -3.3318681318681316 22 17 -2.905677655677656 22 18 -2.677545787545787 22 19 -2.955677655677656 22 20 -2.6237728937728946 22 21 -2.555824175824176 22 22 -2.2691208791208783 22 23 -2.2825274725274722 22 24 -2.07084249084249 22 25 -2.107728937728937 22 26 -2.178571428571428 22 27 -2.426630036630037 22 28 -2.358205128205129 22 29 -2.5439926739926753 22 30 -2.44054945054945 22 31 -2.7452014652014647 22 32 -2.5663369963369966 22 33 -2.4700366300366294 22 34 -2.4507692307692306 22 35 -2.5082051282051268 22 36 -2.1573626373626382 22 37 -2.071721611721613 22 38 -2.218388278388278 22 39 -2.1063369963369976 22 40 -2.163516483516484 25 1 11.687179487179485 25 2 15.922454212454213 25 3 17.64388278388278 25 4 17.474652014652015 25 5 18.541978021978025 25 6 19.272820512820516 25 7 20.815860805860808 25 8 20.781831501831505 25 9 21.482527472527472 25 10 9.51186813186813 25 11 5.529487179487182 25 12 5.358315018315022 25 13 5.617692307692305 25 14 4.896849816849818 25 15 10.189157509157509 25 16 5.668131868131868 25 17 1.594322344322344 25 18 4.322454212454213 25 19 6.044322344322344 25 20 1.8762271062271054 25 21 6.444175824175824 25 22 1.7308791208791217 25 23 5.717472527472528 25 24 5.42915750915751 25 25 5.892271062271063 25 26 -0.17857142857142794 25 27 4.073369963369963 25 28 -0.8582051282051291 25 29 8.956007326007324 25 30 9.059450549450549 25 31 10.754798534798535 25 32 10.933663003663003 25 33 0.5299633699633706 25 34 0.5492307692307694 25 35 0.49179487179487325 25 36 8.842637362637362 25 37 12.928278388278386 25 38 7.781611721611722 25 39 8.393663003663002 25 40 10.836483516483515 26 1 14.353846153846149 26 2 16.589120879120877 26 3 19.310549450549445 26 4 13.974652014652015 26 5 19.70864468864469 26 6 15.106153846153846 26 7 16.649194139194137 26 8 16.615164835164833 26 9 16.3158608058608 26 10 15.51186813186813 26 11 16.529487179487184 26 12 16.191648351648354 26 13 13.284358974358973 26 14 10.73018315018315 26 15 9.355824175824177 26 16 3.3347985347985345 26 17 8.260989010989011 26 18 6.822454212454213 26 19 10.210989010989012 26 20 14.209560439560438 26 21 9.11084249084249 26 22 2.5642124542124556 26 23 10.217472527472527 26 24 15.095824175824177 26 25 5.558937728937729 26 26 6.154761904761906 26 27 8.573369963369963 26 28 8.141794871794872 26 29 8.456007326007324 26 30 8.059450549450549 26 31 12.588131868131867 26 32 12.600329670329671 26 33 12.02996336996337 26 34 12.54923076923077 26 35 12.491794871794873 26 36 12.675970695970694 26 37 12.261611721611718 26 38 11.94827838827839 26 39 12.06032967032967 26 40 12.003150183150183 27 1 -7.812820512820515 27 2 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-2.2691208791208783 119 23 -2.2825274725274722 119 24 -2.07084249084249 119 25 -2.107728937728937 119 26 -2.178571428571428 119 27 -2.426630036630037 119 28 -2.358205128205129 119 29 -2.5439926739926753 119 30 -2.44054945054945 119 31 -2.7452014652014647 119 32 -2.5663369963369966 119 33 -2.4700366300366294 119 34 -2.4507692307692306 119 35 -2.5082051282051268 119 36 -2.1573626373626382 119 37 -2.071721611721613 119 38 -2.218388278388278 119 39 -2.1063369963369976 119 40 -2.163516483516484 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 645.0 5 75.0 6 74.0 7 74.0 8 58.0 9 65.0 10 106.0 11 93.0 12 69.0 13 45.0 14 36.0 15 46.0 16 39.0 17 25.0 18 25.0 19 22.0 20 26.0 21 29.0 22 45.0 23 33.0 24 36.0 25 33.0 26 20.0 27 14.0 28 16.0 29 10.0 30 2.0 31 5.0 32 0.0 33 0.0 34 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 96.65911664779162 0.8493771234428085 1.4156285390713477 1.0758776896942241 8 94.85002829654782 1.6411997736276174 1.414827391058291 2.0939445387662703 9 88.88888888888889 3.741496598639456 3.798185941043084 3.571428571428571 10 30.560271646859082 35.93661573288059 11.318619128466327 22.184493491794 11 28.07017543859649 20.99603848330504 25.24052065647991 25.693265421618563 12 32.597623089983024 15.336728919071874 25.46689303904924 26.59875495189587 13 21.279003961516697 21.44878324844369 32.48443689869836 24.78777589134126 14 22.76330690826727 22.706681766704417 28.765571913929783 25.76443941109853 15 22.58064516129032 22.52405206564799 30.447085455574417 24.448217317487266 16 27.05149971703452 21.958121109224674 25.52348613469157 25.46689303904924 17 21.279003961516697 22.976796830786643 28.636106395019805 27.108092812676855 18 19.807583474816074 25.86304470854556 26.089417091114885 28.239954725523486 19 19.12599318955732 21.282633371169126 27.128263337116916 32.46311010215664 20 19.35483870967742 21.675155631013016 28.862478777589136 30.107526881720432 21 20.48670062252405 22.976796830786643 31.975099037917374 24.561403508771928 22 16.411997736276174 24.052065647990943 32.371250707413694 27.164685908319186 23 17.374080362195812 20.260328239954724 32.2014714204867 30.164119977362763 24 18.61912846632711 26.032823995472555 27.67402376910017 27.67402376910017 25 18.742921857304644 20.44167610419026 27.519818799546997 33.2955832389581 26 18.902093944538766 20.31692133559706 26.37238256932654 34.40860215053764 27 17.861205915813425 20.932878270762227 30.204778156996586 31.00113765642776 28 18.12004530011325 19.59229898074745 29.728199320498298 32.559456398641 29 17.56373937677054 21.019830028328613 30.934844192634557 30.481586402266288 30 18.675721561969443 17.487266553480477 33.446519524618 30.390492359932086 31 16.24221844934918 18.449349179400112 33.446519524618 31.861912846632713 32 15.402038505096263 19.988674971687427 32.16308040770102 32.44620611551529 33 17.044167610419024 14.665911664779163 35.22083805209513 33.069082672706685 34 15.223542727787208 16.97792869269949 38.256932654216186 29.541595925297116 35 13.242784380305602 18.449349179400112 38.93604980192416 29.37181663837012 36 16.58177702320317 18.902093944538766 40.407470288624786 24.108658743633278 37 15.44159544159544 20.284900284900285 37.66381766381766 26.609686609686612 38 17.10079275198188 19.932049830124576 38.4484711211778 24.518686296715742 39 18.39275608375778 17.374080362195812 37.5778155065082 26.6553480475382 40 14.204867006225241 19.524617996604416 38.08715336728919 28.183361629881155 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 3.0 12 5.0 13 5.0 14 5.0 15 5.0 16 10.0 17 15.0 18 15.0 19 15.5 20 16.0 21 18.0 22 20.0 23 20.0 24 27.0 25 34.0 26 38.5 27 43.0 28 43.0 29 43.5 30 44.0 31 49.0 32 54.0 33 54.0 34 54.5 35 55.0 36 66.5 37 78.0 38 78.0 39 86.0 40 94.0 41 94.5 42 95.0 43 95.0 44 109.5 45 124.0 46 132.0 47 140.0 48 140.0 49 136.5 50 133.0 51 134.5 52 136.0 53 136.0 54 130.5 55 125.0 56 116.5 57 108.0 58 108.0 59 104.0 60 100.0 61 96.5 62 93.0 63 93.0 64 82.0 65 71.0 66 65.5 67 60.0 68 60.0 69 49.5 70 39.0 71 33.5 72 28.0 73 28.0 74 26.0 75 24.0 76 18.5 77 13.0 78 13.0 79 10.5 80 8.0 81 4.5 82 1.0 83 1.0 84 2.0 85 3.0 86 1.5 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.056593095642331635 8 0.0 9 0.1697792869269949 10 0.0 11 0.0 12 0.0 13 0.0 14 0.056593095642331635 15 0.0 16 0.0 17 0.0 18 0.0 19 0.2829654782116582 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.056593095642331635 26 0.0 27 0.5093378607809848 28 0.056593095642331635 29 0.11318619128466327 30 0.0 31 0.0 32 0.056593095642331635 33 0.056593095642331635 34 0.0 35 0.0 36 0.0 37 0.6791171477079796 38 0.056593095642331635 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1767.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.03791737408037 #Duplication Level Percentage of deduplicated Percentage of total 1 99.2 98.24561403508771 2 0.7428571428571429 1.4714204867006226 3 0.0 0.0 4 0.0 0.0 5 0.05714285714285715 0.2829654782116582 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTAGGGGGATTTCTTGTTCTTTACTGTGGGAATTTTGCT 5 0.2829654782116582 No Hit TTTAGGGGGCGCGGAAATGATAGGACAATTTCCAGTATTT 2 0.11318619128466327 No Hit TTTAGGGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC 2 0.11318619128466327 No Hit TTTAGGGGGAATGGACCCTCATTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.11318619128466327 No Hit TTTAGGGGGGTGTAACATTGGTGAAGGATCCAGGAATATG 2 0.11318619128466327 No Hit TTTAGGGGGTGACAAAGGCTGAGGTAAGGTTACTAGGCAG 2 0.11318619128466327 No Hit TTTAGGGGGCTGCTGCCCTGGAGATGCCGTCACCTTCCAC 2 0.11318619128466327 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGC 30 8.064844E-6 34.0 4 TTTAGGG 185 0.0 32.162163 1 TTAGGGG 175 0.0 32.05714 2 TAGGGGG 160 0.0 31.875 3 AGGGGGG 60 1.2732926E-11 31.166666 4 AGGGGGA 55 2.3464963E-10 30.90909 4 GGGGGGA 35 2.3241171E-5 29.142857 5 GGGGGAT 25 0.0062703886 27.199999 5 >>END_MODULE