FastQCFastQC Report
Thu 26 May 2016
SRR1926999_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926999_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013531
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47770.15851836267820044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG3350.010.8027464
CTAATAC2050.010.6964653
GTATTAG1701.2732926E-1010.6646841
GTATAAG2900.010.5291981
CCGCGTC750.00268881710.1150339
GTCCTAG3650.09.9342261
GTATCAA32750.09.7606071
TAGCCGT800.004426489.5234744
TAGATCG800.00444416759.5188715
ACTGCGC1304.1825533E-69.5104728
TTTGCGA800.00458412349.48315810
GGCGTGC1951.6516424E-99.2666148
TAGTACC1554.114172E-79.2162654
GTCTTAC2553.6379788E-128.9807871
GTACTAG1701.5758633E-78.9807861
TTAGGAC3450.08.8429493
GTCCTAA4450.08.7915841
GTCCTAC6200.08.7725631
TGCGTTG6200.08.71679612
CTTAGAC3400.08.6925873
Produced by FastQC (version 0.11.5)