##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926999_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3013531 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.02763701451885 31.0 28.0 31.0 16.0 34.0 2 28.01285734243318 31.0 28.0 31.0 16.0 34.0 3 27.65947587730141 31.0 28.0 31.0 16.0 34.0 4 31.47310712914518 35.0 32.0 37.0 17.0 37.0 5 31.670166326478807 35.0 32.0 37.0 17.0 37.0 6 31.125275299972028 35.0 32.0 37.0 11.0 37.0 7 30.919962993577965 35.0 32.0 37.0 11.0 37.0 8 30.775957838163933 35.0 32.0 37.0 10.0 37.0 9 32.83772192819652 37.0 32.0 39.0 17.0 39.0 10 33.03322779822076 37.0 33.0 39.0 17.0 39.0 11 33.198456893259106 38.0 33.0 39.0 17.0 39.0 12 33.077921216008725 38.0 33.0 39.0 16.0 39.0 13 33.16218913958409 38.0 33.0 39.0 17.0 39.0 14 34.16913182575524 39.0 34.0 41.0 17.0 41.0 15 33.717898040537825 39.0 33.0 41.0 10.0 41.0 16 33.79715158065406 39.0 33.0 41.0 11.0 41.0 17 34.02446863828512 39.0 34.0 41.0 15.0 41.0 18 34.079491798823376 39.0 34.0 41.0 11.0 41.0 19 33.42987113787779 39.0 33.0 40.0 10.0 41.0 20 33.71198736631546 39.0 33.0 41.0 10.0 41.0 21 33.69803330378881 39.0 34.0 41.0 2.0 41.0 22 33.710632145479835 39.0 34.0 41.0 2.0 41.0 23 33.69811228090901 39.0 34.0 41.0 2.0 41.0 24 32.373937085764176 38.0 32.0 40.0 2.0 41.0 25 32.93824188302692 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.24539929273209182 1101 2 -0.3928123911217831 1101 3 -1.2938127169066505 1101 4 -0.6060060394467364 1101 5 -0.38199553567461564 1101 6 -2.4136679117692665 1101 7 -0.8807824807272624 1101 8 -0.21742235683688094 1101 9 -0.2492362746958534 1101 10 -9.07146480328598 1101 11 -3.9279645540119823 1101 12 -1.5905546011544978 1101 13 -0.9363478418818971 1101 14 -0.7154296568463607 1101 15 -2.009516541431694 1101 16 -0.5010051167666703 1101 17 -0.4137789556556797 1101 18 -0.31711994528124876 1101 19 -1.0932700000420397 1101 20 -0.26478961340521323 1101 21 -0.28733829122136 1101 22 -0.08756545642429359 1101 23 -0.2599328503191245 1101 24 -0.15995000031315243 1101 25 -0.14078844635869814 1102 1 -0.3772582746370894 1102 2 -0.5129075835803647 1102 3 -0.7705186752308038 1102 4 -0.5255795884806744 1102 5 -0.2871204639739169 1102 6 -0.9960475364450403 1102 7 -0.4050569579266714 1102 8 0.18469624804842155 1102 9 0.06772879037384882 1102 10 0.07719608066187789 1102 11 -0.1136947372937982 1102 12 -0.11191035063962573 1102 13 -0.13838391078394352 1102 14 -0.38737401146002526 1102 15 0.19623256908965203 1102 16 -0.07739339058128536 1102 17 -0.2227133372911041 1102 18 -0.36035383688364675 1102 19 0.15603044027645296 1102 20 0.0017525035001000333 1102 21 -0.03375936287751813 1102 22 -0.11412486257573562 1102 23 -0.5652301887132722 1102 24 -4.002895426498817 1102 25 -2.1585562616152103 1103 1 -0.28250425243614785 1103 2 -0.6191324753528598 1103 3 -0.061841360709799176 1103 4 -0.16078201476982912 1103 5 -0.3570533095284105 1103 6 0.3290990799420257 1103 7 0.10642135475702119 1103 8 0.29925938957103426 1103 9 0.07923454953355247 1103 10 0.08554854468864903 1103 11 -0.1874285321138629 1103 12 -0.24455513752006652 1103 13 -0.14885058047610045 1103 14 -0.11398793402870666 1103 15 0.24372236401433867 1103 16 0.14922296133649837 1103 17 -0.12734164015919447 1103 18 -0.23837761411212455 1103 19 -1.8452006194583497 1103 20 -0.5907360214435826 1103 21 -0.40643660792947855 1103 22 -0.3606018153408215 1103 23 -0.3343958148087367 1103 24 1.146349206164473 1103 25 0.4305186143355044 1104 1 -0.4129830389968028 1104 2 -0.2797366929081342 1104 3 -1.5554788433456679 1104 4 -0.5874433111720592 1104 5 -0.36427839860426303 1104 6 -1.7696302298598 1104 7 -0.2902520764033234 1104 8 0.28770700664956905 1104 9 0.11954004883088487 1104 10 0.20502597109071985 1104 11 0.04782577155169321 1104 12 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0.31922337600540374 2215 4 0.567719124253415 2215 5 0.47399423512734273 2215 6 0.9889385167120999 2215 7 0.5947226031117694 2215 8 -0.7258659091207313 2215 9 -0.11608832505591948 2215 10 -0.29932250434777075 2215 11 -0.34245739137185893 2215 12 -0.6108889130935253 2215 13 -0.2482702925943414 2215 14 -0.08636639037547411 2215 15 -0.26328794692660296 2215 16 -0.04825446438946557 2215 17 -0.45547814800359987 2215 18 -1.142825830515939 2215 19 0.06942233365452921 2215 20 -4.0277576906255845 2215 21 -2.268393617350796 2215 22 -1.2154215564002655 2215 23 -1.1350528556166282 2215 24 -0.8556003374417855 2215 25 -0.6770018314186999 2216 1 -0.35679493405907436 2216 2 -0.7076029550865925 2216 3 0.09060409924147095 2216 4 -0.26536946936128913 2216 5 -0.39250047371713137 2216 6 -1.7867535918877913 2216 7 -9.429385602312387 2216 8 -10.837866412482274 2216 9 -5.6925155001610115 2216 10 -3.3323386586114943 2216 11 -2.2287241848885166 2216 12 -1.8488956662871772 2216 13 -1.525398730376093 2216 14 -1.5899055881231803 2216 15 -2.57600029581673 2216 16 -1.560796225639244 2216 17 -1.866569914310432 2216 18 -1.6200132582320848 2216 19 -0.9880191510168359 2216 20 -1.915723762038379 2216 21 -4.5506017879975715 2216 22 -3.173312731149114 2216 23 -2.9603596456429315 2216 24 -3.1670128885599347 2216 25 -2.5993380491651195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 189016.0 3 10.0 4 1438.0 5 8466.0 6 11009.0 7 9048.0 8 7212.0 9 6465.0 10 6064.0 11 5945.0 12 5840.0 13 5806.0 14 5735.0 15 5940.0 16 6367.0 17 6864.0 18 8128.0 19 9866.0 20 12696.0 21 16230.0 22 20469.0 23 24144.0 24 28231.0 25 32090.0 26 36766.0 27 40180.0 28 45225.0 29 53298.0 30 63334.0 31 77028.0 32 98583.0 33 129010.0 34 173989.0 35 239858.0 36 344777.0 37 545000.0 38 733404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.535945343325125 17.451428020358247 12.197856098911423 25.814770537405206 2 16.140669724511543 19.62388840250586 38.74308288575942 25.492358987223167 3 18.946397866879312 23.462799139441966 28.78114746608853 28.809655527590188 4 12.821411102370389 16.574490602026202 36.03855555153386 34.565542744069546 5 14.032197196627402 36.81040442820154 35.04248937309297 14.114909002078086 6 33.98130434147656 35.066441146911345 17.464615392785404 13.487639118826694 7 29.70902380375417 30.89984395346298 21.14417262049003 18.24695962229282 8 26.52935627808765 34.897920872949776 20.02946312017505 18.54325972878752 9 26.8661907905458 15.29448195451065 19.045433258689354 38.79389399625419 10 15.443898040980924 28.244245667777403 33.36078628985409 22.951070001387585 11 34.9994075772365 22.494209357890057 22.998714591953473 19.507668472919974 12 23.562593324356715 24.51565924209421 30.04676713973687 21.874980293812207 13 28.79843534635685 21.411633278162455 25.045742488623702 24.744188886856996 14 22.299148059261807 20.806716754015845 26.465888019361415 30.428247167360933 15 24.418194423839815 28.53926401482604 22.840936358413675 24.20160520292047 16 23.856671318830312 27.4395232555631 25.246678073041736 23.457127352564857 17 23.03230829891549 27.464476935066106 26.170903679157988 23.332311086860415 18 23.082442605203052 27.231090599048546 26.7592475203098 22.927219275438603 19 24.435754193652674 26.608497600426446 26.321192687134044 22.63455551878684 20 24.38820478337667 26.38301530226504 26.464030417342343 22.76474949701595 21 24.34581889627889 26.008469738606344 26.21473707966599 23.430974285448777 22 24.28619695348448 26.122529717731336 26.04838474140197 23.542888587382212 23 23.856497985564566 26.22144750602638 26.040465276669156 23.881589231739895 24 24.076626546810463 26.360753454550284 26.050935014462066 23.51168498417719 25 23.910357359176256 26.006853463654238 26.254640193158153 23.82814898401135 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1132.0 1 1132.0 2 1428.0 3 1724.0 4 1724.0 5 1724.0 6 2331.0 7 2938.0 8 2938.0 9 2938.0 10 4028.0 11 5118.0 12 5118.0 13 5118.0 14 7796.0 15 10474.0 16 10474.0 17 10474.0 18 18092.0 19 25710.0 20 25710.0 21 25710.0 22 39991.5 23 54273.0 24 54273.0 25 54273.0 26 76617.5 27 98962.0 28 98962.0 29 98962.0 30 128099.0 31 157236.0 32 157236.0 33 157236.0 34 190251.5 35 223267.0 36 223267.0 37 223267.0 38 257911.0 39 292555.0 40 292555.0 41 292555.0 42 323096.5 43 353638.0 44 353638.0 45 353638.0 46 378952.0 47 404266.0 48 404266.0 49 404266.0 50 405853.5 51 407441.0 52 407441.0 53 407441.0 54 380988.5 55 354536.0 56 354536.0 57 354536.0 58 310792.0 59 267048.0 60 267048.0 61 267048.0 62 221975.5 63 176903.0 64 176903.0 65 176903.0 66 138588.5 67 100274.0 68 100274.0 69 100274.0 70 73688.0 71 47102.0 72 47102.0 73 47102.0 74 33149.5 75 19197.0 76 19197.0 77 19197.0 78 12988.5 79 6780.0 80 6780.0 81 6780.0 82 4484.0 83 2188.0 84 2188.0 85 2188.0 86 1390.0 87 592.0 88 592.0 89 592.0 90 361.5 91 131.0 92 131.0 93 131.0 94 81.0 95 31.0 96 31.0 97 31.0 98 23.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21224271460953945 2 0.12563335170602194 3 0.12795620818236147 4 0.06915475566702317 5 0.021901218205487183 6 0.02120436126258532 7 0.30857489104973534 8 0.3018717909322983 9 0.018649219138611815 10 0.036667948662217176 11 0.01592815869489977 12 0.017189137924912667 13 0.015529954727527275 14 0.017520974564389745 15 0.015065383432259367 16 0.01977746371283388 17 0.018549668146768693 18 0.01503219976831166 19 0.02336129941918633 20 0.017023219605174132 21 0.014965832440416243 22 0.017089586933069546 23 0.017520974564389745 24 0.015795424039108937 25 0.015131750760154784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3013531.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.25108862322719 #Duplication Level Percentage of deduplicated Percentage of total 1 88.89692971120509 71.34075384586717 2 7.63008716143319 12.246456019902457 3 1.7371175272781307 4.182167178916756 4 0.6642839745233038 2.13238048441837 5 0.32994168514107053 1.3239089707376486 6 0.18875880848889676 0.9088859921074333 7 0.12265156313593403 0.689004502409943 8 0.08349601611768111 0.5360516951317146 9 0.05595171911650368 0.4041167732496406 >10 0.2730449955135369 3.76083310706765 >50 0.011419204363911738 0.626836560414085 >100 0.005120657453700625 0.7595316976555241 >500 8.255008484015007E-4 0.439087380770639 >1k 3.7147538054247336E-4 0.6499857913509756 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4777 0.15851836267820044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.318366394770785E-5 0.0 0.0 0.0 0.0 7 3.318366394770785E-5 0.0 0.0 0.0 0.0 8 6.63673278954157E-5 0.0 0.0 0.0 0.0 9 6.63673278954157E-5 0.0 0.0 0.0 0.0 10 6.63673278954157E-5 0.0 0.0 0.0 0.0 11 6.63673278954157E-5 0.0 0.0 0.0 0.0 12 6.63673278954157E-5 0.0 0.0 0.0 3.318366394770785E-5 13 6.63673278954157E-5 0.0 0.0 0.0 3.318366394770785E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 335 0.0 10.802746 4 CTAATAC 205 0.0 10.696465 3 GTATTAG 170 1.2732926E-10 10.664684 1 GTATAAG 290 0.0 10.529198 1 CCGCGTC 75 0.002688817 10.115033 9 GTCCTAG 365 0.0 9.934226 1 GTATCAA 3275 0.0 9.760607 1 TAGCCGT 80 0.00442648 9.523474 4 TAGATCG 80 0.0044441675 9.518871 5 ACTGCGC 130 4.1825533E-6 9.510472 8 TTTGCGA 80 0.0045841234 9.483158 10 GGCGTGC 195 1.6516424E-9 9.266614 8 TAGTACC 155 4.114172E-7 9.216265 4 GTCTTAC 255 3.6379788E-12 8.980787 1 GTACTAG 170 1.5758633E-7 8.980786 1 TTAGGAC 345 0.0 8.842949 3 GTCCTAA 445 0.0 8.791584 1 GTCCTAC 620 0.0 8.772563 1 TGCGTTG 620 0.0 8.716796 12 CTTAGAC 340 0.0 8.692587 3 >>END_MODULE