FastQCFastQC Report
Thu 26 May 2016
SRR1926999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926999_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013531
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81620.2708450651411915No Hit
GGTATCAACGCAGAGTACTTTTTTT52430.17398195007783226No Hit
TATCAACGCAGAGTACTTTTTTTTT45530.15108522195391386No Hit
GTACATGGGAAGCAGTGGTATCAAC30340.10067923641734564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG1459.276846E-1111.8220295
GCGTAAG1002.6588654E-49.5342591
GGTATCA23200.09.5342581
CGTAAGG1106.5822285E-59.5334642
GACCGCT1304.3019136E-69.4894787
GACCGTG2304.3655746E-119.0768917
GTATTAA2651.8189894E-128.9945841
CTAGGAC3400.08.9641523
GGTCGTA850.00751934348.92934711
CCCGTTT850.00752241358.92890214
GTATCAA41600.08.9154491
TAGGACG4250.08.739034
GTATAGA2950.08.7262711
ACCGTGC1856.718619E-88.7184698
TGCGTTG7200.08.69637912
CCTAGTA2201.5043042E-98.6667862
ATACCGA1106.975133E-48.6575476
CCCCTAA2659.094947E-128.6348011
GTACTGT4750.08.6210946
AGGACCG1909.7277734E-88.5208485
Produced by FastQC (version 0.11.5)