FastQCFastQC Report
Thu 26 May 2016
SRR1926998_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926998_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361236
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7720.2137107043594769No Hit
GGTATCAACGCAGAGTACTTTTTTT4670.12927836649724833No Hit
CACACACACACACACACACACACAC3770.10436390614445958No Hit
TATCAACGCAGAGTACTTTTTTTTT3740.10353342413269996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAC350.00210788716.351961
GTATTAG651.8155151E-716.1423191
ATTATAC456.5885135E-414.823443
GCTGAAT456.700584E-414.7905188
CCTAGAC400.00516191314.2940323
TAGGACC1203.6379788E-1214.2840964
TCATAGC551.9030966E-413.8608793
GTAGGAC1900.013.5417153
GTCCTAC1550.013.538721
GTCTAGG500.001450569813.35411
CTAGGAC500.001461266213.3410973
CTACACT1009.644282E-913.3318214
GACGTGG500.001476664313.3225617
GTCTTAG655.2268082E-513.2073531
AGGACCA655.3371834E-513.1779915
GACTGTG655.34415E-513.176167
AGTGTGC1600.012.4794998
TCCTACA1700.012.3338212
AAGAGTG701.0679739E-412.2367055
GCATTTC1405.2750693E-1112.19265813
Produced by FastQC (version 0.11.5)