FastQCFastQC Report
Thu 26 May 2016
SRR1926998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926998_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361236
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12870.35627678304487925No Hit
GGTATCAACGCAGAGTACTTTTTTT9180.25412749559844533No Hit
TATCAACGCAGAGTACTTTTTTTTT5980.16554274767741864No Hit
CACACACACACACACACACACACAC5220.1445038700461748No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4150.11488334496008151No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4120.11405286294832188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT453.4571553E-516.928164
TCAAGAC350.002130523616.325853
TAGGACC952.910383E-1015.0348794
GTATAGG456.557232E-414.8327461
CATACTG456.6267565E-414.81213955
GTCCTAT400.00514046214.3030051
CCTATAC602.4987792E-514.2851183
GGCGAGG400.005313767614.23172819
AAGACTG551.9154612E-413.8503125
TGGCGAG1101.8735591E-1013.80046318
GTAGGAC1750.013.6048763
CTATACT707.0542465E-613.6029854
GTCTTAG500.001454370813.349471
GTGCTGA603.9526715E-412.7137811
CTAAATT751.4906942E-512.65042419
ACTGGGG751.4906942E-512.6504248
ATACTGG701.0631135E-412.2426866
GTTCTAT550.00297662112.1358831
GGACGTG1103.6568963E-812.1190236
GACTGTC550.003090164212.0754057
Produced by FastQC (version 0.11.5)