##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926998_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 361236 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.01123088507236 31.0 30.0 33.0 26.0 34.0 2 30.31855075352401 31.0 30.0 34.0 26.0 34.0 3 30.366679954378856 31.0 30.0 34.0 26.0 34.0 4 33.95668482653999 37.0 35.0 37.0 30.0 37.0 5 33.93722109645772 37.0 35.0 37.0 30.0 37.0 6 33.88565923662093 37.0 35.0 37.0 30.0 37.0 7 33.895027073713585 37.0 35.0 37.0 30.0 37.0 8 33.90477416425827 37.0 35.0 37.0 30.0 37.0 9 35.457487625818025 39.0 35.0 39.0 30.0 39.0 10 35.34953880565614 39.0 35.0 39.0 30.0 39.0 11 35.37729074621577 39.0 35.0 39.0 30.0 39.0 12 35.27480372941789 39.0 35.0 39.0 30.0 39.0 13 35.285627678304486 39.0 35.0 39.0 30.0 39.0 14 36.40509251569611 40.0 37.0 41.0 30.0 41.0 15 36.379757277790695 40.0 36.0 41.0 30.0 41.0 16 36.353597094420266 40.0 36.0 41.0 30.0 41.0 17 36.34726882149066 40.0 36.0 41.0 30.0 41.0 18 36.32583131249377 40.0 36.0 41.0 29.0 41.0 19 36.40643236000842 40.0 37.0 41.0 30.0 41.0 20 36.36671317365933 40.0 37.0 41.0 29.0 41.0 21 36.32688879292208 40.0 37.0 41.0 29.0 41.0 22 36.24582544375422 40.0 37.0 41.0 29.0 41.0 23 36.18420367848166 40.0 37.0 41.0 29.0 41.0 24 36.12057768328738 40.0 37.0 41.0 29.0 41.0 25 36.01404621909223 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1814629353726609 1101 2 -0.0440293022891467 1101 3 0.265525722261156 1101 4 0.07124493717065405 1101 5 0.19818660211738148 1101 6 0.31193922669709195 1101 7 0.33310866004231343 1101 8 0.3660143733782917 1101 9 0.4427786078483251 1101 10 0.5471670453218209 1101 11 0.43921927230738333 1101 12 0.6203178267128351 1101 13 0.31223009906853605 1101 14 0.6261604268557122 1101 15 0.374353439235918 1101 16 0.3677468864786988 1101 17 0.4387571682645586 1101 18 0.36573765162308547 1101 19 0.153420763954486 1101 20 0.24403265563880439 1101 21 0.375035268464849 1101 22 0.34629649858938905 1101 23 0.5069707773232679 1101 24 0.4860118343783384 1101 25 0.6266343102258602 1102 1 -0.36251316013451174 1102 2 -0.3778612229583622 1102 3 -0.06056424505367275 1102 4 -0.02950086638593774 1102 5 -0.31920855604560217 1102 6 -0.4302010979386637 1102 7 0.01913713491905611 1102 8 -0.06438075708539515 1102 9 0.0024844413201563498 1102 10 -0.16272091102408837 1102 11 -0.08985113930416588 1102 12 -0.09054306742044815 1102 13 0.17313808200576375 1102 14 0.016266930650516542 1102 15 -0.1485608037423134 1102 16 -0.28763577930158846 1102 17 -0.2919225724700283 1102 18 -0.2619457177193354 1102 19 -0.15528097002146524 1102 20 -0.25148352075031255 1102 21 0.2056263714508333 1102 22 -0.14334050213079053 1102 23 0.037613124007663146 1102 24 -0.06820261357468382 1102 25 -0.057919177115564935 1103 1 -0.34357279933288254 1103 2 -0.2732459975435759 1103 3 0.10477672283291284 1103 4 -0.026889711199842736 1103 5 -0.19466645677341887 1103 6 -0.20751474700216477 1103 7 -0.4374459425877575 1103 8 -0.2292043230196512 1103 9 -0.38509446247757495 1103 10 -0.2072140295781253 1103 11 0.01729245698434312 1103 12 -0.07170618700929055 1103 13 -0.28808436634027146 1103 14 -0.44754597680351793 1103 15 -0.36239613194761944 1103 16 -0.10851812209762812 1103 17 -0.24151727427403813 1103 18 0.008569269690553938 1103 19 0.07221721907169609 1103 20 0.1041026956616804 1103 21 0.1555384131189399 1103 22 0.0422584768626848 1103 23 -0.29178565492372854 1103 24 -0.019977302271172448 1103 25 -0.03432909743565915 1104 1 0.10000969178505059 1104 2 0.06455193974730733 1104 3 0.403786760430787 1104 4 0.1569707972338037 1104 5 0.19815890282094273 1104 6 0.13652650718016446 1104 7 0.19646803071429986 1104 8 0.10929383158005379 1104 9 -0.11634554039830647 1104 10 0.09687689519163456 1104 11 -0.05877252870087091 1104 12 0.020974300038417937 1104 13 0.11463716792898282 1104 14 0.3032870403397325 1104 15 0.21341360210777793 1104 16 0.2059656832212582 1104 17 0.15049059023564126 1104 18 0.15670144864264302 1104 19 0.13722360036244652 1104 20 0.15071026352756434 1104 21 0.13798468954955467 1104 22 0.06141269713794628 1104 23 0.125364495122831 1104 24 0.13106612499935721 1104 25 0.09381756877109382 1105 1 0.22059179174835464 1105 2 0.07340178982548906 1105 3 0.33973526798968834 1105 4 0.4001019978315128 1105 5 0.17892527272946523 1105 6 0.13131974069786168 1105 7 0.05114800466393632 1105 8 0.01551914076280525 1105 9 0.14954336208143815 1105 10 0.25824367965794437 1105 11 -0.03503959119198896 1105 12 0.2909162156656606 1105 13 0.24426747097932378 1105 14 0.2286831678278176 1105 15 0.20714564702812055 1105 16 0.2506480043481716 1105 17 0.3369159714866541 1105 18 0.3508361501709487 1105 19 0.42109981486138537 1105 20 0.47633648050592114 1105 21 0.4805205005323643 1105 22 0.4525317717550408 1105 23 0.3157205718325571 1105 24 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0.41777064449039614 1107 11 0.4517179048421127 1107 12 0.45236574269577545 1107 13 0.14947438306371907 1107 14 0.36111147159917323 1107 15 0.31080732830713487 1107 16 0.3903715993109955 1107 17 0.5631418088946845 1107 18 0.11322835285927368 1107 19 0.13628642416671966 1107 20 0.2167448862481507 1107 21 0.21558881840468302 1107 22 0.1390366254901636 1107 23 0.08380865064128784 1107 24 0.054125279296840745 1107 25 0.19696304887590088 1108 1 0.6015707031916975 1108 2 0.46002532852604716 1108 3 0.6247873526495482 1108 4 0.47049269645362557 1108 5 0.363252756620156 1108 6 0.45065028090110104 1108 7 0.5709981180021941 1108 8 0.28798940538853657 1108 9 0.2324713095128601 1108 10 0.3439286757102096 1108 11 0.3838949018421687 1108 12 0.4240950354196258 1108 13 0.26234746996055236 1108 14 0.17318880927142288 1108 15 0.4438156671488471 1108 16 0.26139285318804184 1108 17 0.4177904589175654 1108 18 0.44498967893849084 1108 19 0.6063123559305907 1108 20 0.17667776874166918 1108 21 0.14674888348832837 1108 22 0.290305248055887 1108 23 0.09179792200956882 1108 24 -0.10639106788372032 1108 25 0.19316226583832474 1109 1 0.5101511331398072 1109 2 0.7065289114269611 1109 3 0.7161636807938514 1109 4 0.7620543470590064 1109 5 0.4079154697409848 1109 6 0.660144968323884 1109 7 0.5624547525463015 1109 8 0.4874457928360272 1109 9 0.340625497800481 1109 10 0.41512956525802025 1109 11 0.3075605801223702 1109 12 0.4620243657877978 1109 13 0.44819501134923456 1109 14 0.697472229088568 1109 15 0.5006293563013031 1109 16 0.5382213681373926 1109 17 0.515152702554829 1109 18 0.4599760886246784 1109 19 0.42220066826070735 1109 20 0.40820729678713263 1109 21 0.5522282509209901 1109 22 0.6621856532943298 1109 23 0.8119548283599514 1109 24 0.625206411730808 1109 25 0.7046646132561634 1110 1 0.1957821657386063 1110 2 0.11839074594405119 1110 3 0.3833038495548564 1110 4 0.658988724525166 1110 5 0.256485857648471 1110 6 0.05975038324527304 1110 7 0.3489417312387104 1110 8 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0.32284886350363706 2214 11 0.4769465450346573 2214 12 0.3322875236825382 2214 13 0.30889676573519864 2214 14 0.5072967904920773 2214 15 0.2723837422662143 2214 16 0.432519613751424 2214 17 0.3293632288706192 2214 18 0.3444497728352047 2214 19 0.4144813700150962 2214 20 0.33259326169941517 2214 21 0.5300352684648502 2214 22 0.42288740768029953 2214 23 0.594849565202054 2214 24 0.5338906222571254 2214 25 0.5746646132561608 2215 1 0.7635793897717242 2215 2 0.521920046268896 2215 3 0.34014876427550433 2215 4 0.5873022494236153 2215 5 0.5285189107105595 2215 6 0.4851386118529959 2215 7 0.3358169018809889 2215 8 0.28764919990442195 2215 9 0.4420832484573154 2215 10 0.4864237427306861 2215 11 0.3274859975306299 2215 12 0.32815708889992834 2215 13 0.27640079150009456 2215 14 0.4619183685919168 2215 15 0.5052822929908558 2215 16 0.5045405477288796 2215 17 0.400329412445501 2215 18 0.43270259087063323 2215 19 0.2415667162308779 2215 20 0.3034950330359649 2215 21 0.31867455993022986 2215 22 0.41068128851765806 2215 23 0.6223455394371626 2215 24 0.49631413272411606 2215 25 0.3674665456233157 2216 1 -0.6004532195871484 2216 2 -0.39683245739872675 2216 3 -2.2489393568661953 2216 4 -1.845474772279701 2216 5 -1.0890931143221394 2216 6 -0.5023423868019847 2216 7 -0.7748989281959169 2216 8 -0.4287194142508852 2216 9 -0.5704384057805498 2216 10 -0.7443779349587487 2216 11 -0.7321775625985154 2216 12 -0.7536899612158692 2216 13 -0.5346946675432207 2216 14 -0.8557894258725582 2216 15 -0.9606585421928671 2216 16 -1.2798774208449473 2216 17 -1.0160129380706238 2216 18 -1.069715520959413 2216 19 -0.8696372461298836 2216 20 -0.6833704944785097 2216 21 -0.7578587458130173 2216 22 -0.9898418619517741 2216 23 -0.9888544435843301 2216 24 -0.9165953744479793 2216 25 -0.9924248980013886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7948.0 3 6.0 4 124.0 5 836.0 6 1262.0 7 871.0 8 698.0 9 632.0 10 542.0 11 546.0 12 451.0 13 436.0 14 422.0 15 427.0 16 429.0 17 462.0 18 524.0 19 628.0 20 801.0 21 1077.0 22 1342.0 23 1654.0 24 1949.0 25 2348.0 26 2799.0 27 3161.0 28 3557.0 29 4388.0 30 5521.0 31 7281.0 32 9425.0 33 12392.0 34 16879.0 35 23898.0 36 35502.0 37 61697.0 38 148321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39361640213839 19.905440497074807 11.379011226862437 27.32193187392436 2 13.650234058651856 20.86822040592339 41.80739287208951 23.674152663335246 3 18.594464687185052 24.769361966043878 29.312626670949022 27.323546675822048 4 11.335802633181633 18.046927770211163 38.66004495675957 31.957224639847638 5 12.265305331465557 38.85342207112681 35.77795987973888 13.10331271766875 6 30.419493008449862 39.01546085343022 18.14525313466998 12.419793003449943 7 27.702846763229378 32.37490550939091 21.38215186613761 18.540095861242097 8 24.23134958539253 39.218406079986714 19.606019076096736 16.944225258524025 9 25.409003192287567 16.825542730416437 20.415966421453945 37.349487655842054 10 13.762364451285517 31.227212451241222 34.363277475866305 20.647145621606956 11 33.70578415386021 24.15513370301569 23.534167010417733 18.604915132706367 12 21.90891172432121 26.521459892811265 31.78760464189219 19.78202374097533 13 27.823792887712674 22.90980361759967 25.946062662983 23.320340831704655 14 19.746148629008818 23.558348443533987 28.542915276758897 28.152587650698297 15 22.330403488130663 31.261955844695134 23.924146999792374 22.483493667381826 16 20.899472364173917 30.582669405426955 27.755692985710095 20.762165244689037 17 20.555317175799686 29.74462607443908 28.08338062480103 21.616676124960204 18 20.031392480753418 29.74479075831942 29.75835541049683 20.46546135043033 19 21.5939762204659 28.481182609658536 29.049787534430493 20.87505363544507 20 21.70765602161492 28.551734065642027 29.716913230278603 20.023696682464458 21 22.031124978889167 27.899797612884115 28.54212060721672 21.526956801009998 22 21.08621510591414 29.01316590815976 29.135800418562933 20.764818567363164 23 21.189035362119846 28.347553109618694 28.856918230288404 21.60649329797306 24 20.72252135036749 28.47786067241546 29.90823148366022 20.891386493556826 25 21.39307614578575 28.420192959871542 29.0857245684703 21.101006325872405 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 164.0 2 156.0 3 148.0 4 148.0 5 148.0 6 295.0 7 442.0 8 442.0 9 442.0 10 737.0 11 1032.0 12 1032.0 13 1032.0 14 1643.0 15 2254.0 16 2254.0 17 2254.0 18 3856.5 19 5459.0 20 5459.0 21 5459.0 22 8224.0 23 10989.0 24 10989.0 25 10989.0 26 15053.0 27 19117.0 28 19117.0 29 19117.0 30 24216.0 31 29315.0 32 29315.0 33 29315.0 34 33398.5 35 37482.0 36 37482.0 37 37482.0 38 40462.5 39 43443.0 40 43443.0 41 43443.0 42 45299.5 43 47156.0 44 47156.0 45 47156.0 46 47894.0 47 48632.0 48 48632.0 49 48632.0 50 46360.0 51 44088.0 52 44088.0 53 44088.0 54 37691.5 55 31295.0 56 31295.0 57 31295.0 58 25805.0 59 20315.0 60 20315.0 61 20315.0 62 15788.0 63 11261.0 64 11261.0 65 11261.0 66 8280.5 67 5300.0 68 5300.0 69 5300.0 70 3771.5 71 2243.0 72 2243.0 73 2243.0 74 1499.5 75 756.0 76 756.0 77 756.0 78 506.5 79 257.0 80 257.0 81 257.0 82 161.0 83 65.0 84 65.0 85 65.0 86 41.5 87 18.0 88 18.0 89 18.0 90 10.5 91 3.0 92 3.0 93 3.0 94 2.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11211507158754941 2 0.11875892768162641 3 0.01771694958420534 4 0.0 5 0.006090201419570586 6 0.3404976248214464 7 0.02463763301553555 8 0.01411819419991363 9 0.014671848874420046 10 0.00525971940781096 11 0.006920683431330211 12 0.0011073093490128337 13 5.536546745064169E-4 14 2.7682733725320843E-4 15 0.003045100709785293 16 5.536546745064169E-4 17 2.7682733725320843E-4 18 8.304820117596253E-4 19 2.7682733725320843E-4 20 0.0011073093490128337 21 0.013010884850900796 22 0.0 23 5.536546745064169E-4 24 2.7682733725320843E-4 25 0.0058133740823173765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 361236.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.59567763758389 #Duplication Level Percentage of deduplicated Percentage of total 1 95.57480668663504 86.58664376856787 2 3.592124854540085 6.508619707117329 3 0.39356808720690667 1.0696670267111228 4 0.13902959866058343 0.5038192280934753 5 0.07300024819386439 0.3306753476417476 6 0.048132822152787816 0.2616375383724673 7 0.02880176438252537 0.18265207529750394 8 0.01824222148036663 0.13221331333029632 9 0.019175542694224286 0.15634991560065037 >10 0.09569155658333384 1.622579470969127 >50 0.0097833642092364 0.6143378342920455 >100 0.0064203327392781855 1.1098519816480503 >500 9.17190391325455E-4 0.5644817416018783 >1k 3.0573013044181836E-4 0.35647105075643637 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1287 0.35627678304487925 No Hit GGTATCAACGCAGAGTACTTTTTTT 918 0.25412749559844533 No Hit TATCAACGCAGAGTACTTTTTTTTT 598 0.16554274767741864 No Hit CACACACACACACACACACACACAC 522 0.1445038700461748 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 415 0.11488334496008151 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 412 0.11405286294832188 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTACT 45 3.4571553E-5 16.92816 4 TCAAGAC 35 0.0021305236 16.32585 3 TAGGACC 95 2.910383E-10 15.034879 4 GTATAGG 45 6.557232E-4 14.832746 1 CATACTG 45 6.6267565E-4 14.8121395 5 GTCCTAT 40 0.005140462 14.303005 1 CCTATAC 60 2.4987792E-5 14.285118 3 GGCGAGG 40 0.0053137676 14.231728 19 AAGACTG 55 1.9154612E-4 13.850312 5 TGGCGAG 110 1.8735591E-10 13.800463 18 GTAGGAC 175 0.0 13.604876 3 CTATACT 70 7.0542465E-6 13.602985 4 GTCTTAG 50 0.0014543708 13.34947 1 GTGCTGA 60 3.9526715E-4 12.713781 1 CTAAATT 75 1.4906942E-5 12.650424 19 ACTGGGG 75 1.4906942E-5 12.650424 8 ATACTGG 70 1.0631135E-4 12.242686 6 GTTCTAT 55 0.002976621 12.135883 1 GGACGTG 110 3.6568963E-8 12.119023 6 GACTGTC 55 0.0030901642 12.075405 7 >>END_MODULE