FastQCFastQC Report
Thu 26 May 2016
SRR1926997_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926997_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447115
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9830.21985395256253984No Hit
GGTATCAACGCAGAGTACTTTTTTT6190.13844312984355256No Hit
TATCAACGCAGAGTACTTTTTTTTT4950.11070977265356786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC307.608116E-419.0379524
ATGACGA250.006085062418.96766917
TAGGACC651.861572E-716.1090374
GTAGGAC2150.014.1852993
GGACTGG707.1036193E-613.5954856
GTTCTAG655.2232306E-513.2097851
TGTAGGA2250.013.1312522
ACGTGGA1001.4151738E-712.3621778
TAGGACG1405.0931703E-1112.2386844
GGACGTG1405.0931703E-1112.2359366
TCGAACT701.1049771E-412.19350219
TCTAAAC550.002989296612.1300423
GTCCTAC1850.011.8610391
CTCGAAC802.905873E-511.85479318
GTGACAC657.7896134E-411.730153
CCTACAC907.164579E-611.6486913
GGGACTG752.03376E-411.4202065
ATACAAT1001.8787796E-611.4202066
GACGTGG1104.8254697E-711.2471737
CCCTTAT600.00568210211.1304671
Produced by FastQC (version 0.11.5)