##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926997_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 447115 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.972210728783423 31.0 28.0 31.0 16.0 34.0 2 28.099218321908236 31.0 28.0 31.0 16.0 34.0 3 27.86728246647954 31.0 28.0 31.0 16.0 34.0 4 31.460579492971608 35.0 32.0 37.0 17.0 37.0 5 31.253100432774566 35.0 32.0 37.0 17.0 37.0 6 31.00202632432372 35.0 32.0 37.0 11.0 37.0 7 30.765234894825717 35.0 31.0 37.0 11.0 37.0 8 30.573838945237803 35.0 31.0 37.0 10.0 37.0 9 32.750623441396506 37.0 32.0 39.0 17.0 39.0 10 32.99074511031837 37.0 33.0 39.0 17.0 39.0 11 33.118694295651004 37.0 33.0 39.0 17.0 39.0 12 33.043085112331276 38.0 33.0 39.0 16.0 39.0 13 33.13131968285564 38.0 33.0 39.0 17.0 39.0 14 34.11461257170974 39.0 34.0 40.0 17.0 41.0 15 33.70425505742371 39.0 33.0 40.0 10.0 41.0 16 33.76550328215336 39.0 33.0 40.0 11.0 41.0 17 33.98480480413316 39.0 33.0 40.0 16.0 41.0 18 34.02642496896772 39.0 34.0 41.0 11.0 41.0 19 33.43768158080136 39.0 33.0 40.0 10.0 41.0 20 33.73474385784418 39.0 33.0 41.0 10.0 41.0 21 33.71064938550485 39.0 34.0 41.0 7.0 41.0 22 33.726012323451464 39.0 34.0 41.0 2.0 41.0 23 33.711490332464805 39.0 34.0 41.0 2.0 41.0 24 32.43178824239849 38.0 32.0 40.0 2.0 41.0 25 32.993708553727785 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4677446772715932 1101 2 -0.6034427628187835 1101 3 -1.4482528182671217 1101 4 -0.7362880613942124 1101 5 -0.5309705974950276 1101 6 -2.2754645310425943 1101 7 -0.7713450922368921 1101 8 -0.6211020643116036 1101 9 -0.49695302536639474 1101 10 -9.70799095593593 1101 11 -4.573836992723489 1101 12 -1.904966776795881 1101 13 -1.2606212960557848 1101 14 -1.033113237595792 1101 15 -2.7193043773851997 1101 16 -0.9465756706154238 1101 17 -0.7518954899632959 1101 18 -0.7189707492681734 1101 19 -1.3499962825622447 1101 20 -0.686053703432016 1101 21 -0.7563560258443189 1101 22 -0.4259083020958343 1101 23 -0.4675215686812422 1101 24 -0.6593374156947256 1101 25 -0.5941919025618603 1102 1 0.664373557171885 1102 2 0.5837905175944336 1102 3 0.4778705548815978 1102 4 0.32239577556466514 1102 5 0.6572842327552308 1102 6 0.19465845294428163 1102 7 0.3823428795665613 1102 8 0.9049784125885445 1102 9 0.947043047636825 1102 10 1.1921271193049705 1102 11 0.8893695239684547 1102 12 1.0885435072480405 1102 13 1.007429401118813 1102 14 0.7111831091474059 1102 15 1.2988911499069857 1102 16 1.2169939163865706 1102 17 0.9260332845749062 1102 18 0.9026578045975739 1102 19 1.1439341193800203 1102 20 1.347343458339914 1102 21 1.0306751557483267 1102 22 1.0171015994196893 1102 23 0.49518301767952266 1102 24 -2.929986210398585 1102 25 -1.0959276528260915 1103 1 -0.4166972438157259 1103 2 -0.5315352381276988 1103 3 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22 1.31756266499346 2210 23 1.1578367062041153 2210 24 1.766874817917362 2210 25 1.5331474216895558 2211 1 0.4125167406099699 2211 2 0.3520468514245394 2211 3 0.12439909761470247 2211 4 0.5896335938760764 2211 5 0.7272823698640245 2211 6 -1.393243688570653 2211 7 0.18711188105687526 2211 8 0.935963153079701 2211 9 0.8263600955212311 2211 10 0.9914147659064128 2211 11 0.4785554405109309 2211 12 0.5619649094381529 2211 13 0.5571118996608959 2211 14 0.5243067727254527 2211 15 1.2476474675380373 2211 16 0.25187452935877275 2211 17 0.4038142571661112 2211 18 0.5826637172522737 2211 19 0.8920813525816058 2211 20 0.7956543019866444 2211 21 0.2884486767746992 2211 22 -2.3607352599077274 2211 23 -0.7516865151397596 2211 24 1.3304864779964092 2211 25 0.9117911963931178 2212 1 -0.6276080636776342 2212 2 -0.44712212364471426 2212 3 -2.6918269652541404 2212 4 -1.3318970275860487 2212 5 -0.45078203456810684 2212 6 0.003980959147039442 2212 7 0.40040827994884154 2212 8 -0.033844446583071175 2212 9 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0.7317292457789044 2215 7 0.10079049991477618 2215 8 -0.8694420377511776 2215 9 -0.5756550323729854 2215 10 -0.6731786719364194 2215 11 -0.6451580389047393 2215 12 -0.9421454606755972 2215 13 -0.4895276291802588 2215 14 -0.5034120620802511 2215 15 -0.6588912327333389 2215 16 -0.5289839208981704 2215 17 -1.0698797349812423 2215 18 -2.1775400329642522 2215 19 -0.8695875515121685 2215 20 -4.138621276857403 2215 21 -2.769076656674116 2215 22 -1.666506739207918 2215 23 -1.6516771323809394 2215 24 -1.9117402671967305 2215 25 -1.6727618955867918 2216 1 -1.087523114462492 2216 2 -1.7679531485754616 2216 3 -1.1018472791968925 2216 4 -1.0152395945297243 2216 5 -1.2759676301359768 2216 6 -2.780971949440218 2216 7 -10.211554785609792 2216 8 -12.147768730978271 2216 9 -7.192074687087466 2216 10 -4.378400451484893 2216 11 -3.2843612261557347 2216 12 -2.8401003079531506 2216 13 -2.606627230773885 2216 14 -2.9940627924919383 2216 15 -3.3823706484039917 2216 16 -2.763639963394848 2216 17 -3.064631395007801 2216 18 -2.8312239639071457 2216 19 -2.1910961836502807 2216 20 -3.0372188864191543 2216 21 -5.948996975399215 2216 22 -4.425789607733812 2216 23 -4.311114051371646 2216 24 -4.50289033359779 2216 25 -4.031295760128625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27768.0 3 2.0 4 193.0 5 1294.0 6 1753.0 7 1430.0 8 1145.0 9 935.0 10 892.0 11 874.0 12 823.0 13 886.0 14 858.0 15 924.0 16 931.0 17 1029.0 18 1173.0 19 1474.0 20 1916.0 21 2556.0 22 3143.0 23 3651.0 24 4188.0 25 4921.0 26 5487.0 27 5977.0 28 6762.0 29 8063.0 30 9626.0 31 11221.0 32 14708.0 33 19313.0 34 26032.0 35 36394.0 36 52254.0 37 81865.0 38 104654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.491582484035746 18.875811661022116 12.733075873074931 24.899529981867207 2 13.90035918703702 20.200911851380766 42.944572334536595 22.95415662704562 3 19.145267372673548 24.645895523223654 29.627207764433468 26.581629339669334 4 10.971226241953822 17.533505223860555 39.60286126106769 31.892407273117936 5 12.022648870912175 39.53082564127804 35.918952657506296 12.527572830303491 6 30.883807086385172 38.12276564995817 18.9173105919937 12.07611667166296 7 27.397011349334722 32.782729448820305 22.05593680805289 17.764322393792078 8 24.13561816228148 37.87936228776013 20.723628867089445 17.261390682868942 9 25.792762179176 16.52077232653189 20.53161764870373 37.154847845588385 10 13.372639398550076 30.860332945493603 35.330484202989346 20.436543452966973 11 33.59715094871439 24.152733845458656 23.62614255099256 18.623972654834393 12 21.846100157040656 26.576111029086075 32.341650671785025 19.236138142088237 13 27.94701897867695 22.83615344005297 25.928353481213705 23.288474100056373 14 19.696657867656928 23.278824213681716 29.137398774104067 27.887119144557293 15 22.815918394738606 31.217591660514955 23.889224436838692 22.077265507907747 16 20.85586260740787 30.464574697266954 28.170447707103403 20.509114988221768 17 20.829465046721445 29.699940269926245 28.05322464100436 21.41737004234795 18 20.090463120172338 29.791536642708706 29.62644815930815 20.49155207781081 19 22.15585205866824 28.490474017199567 28.65759259756414 20.69608132656805 20 21.79047516783328 28.64652891027491 29.409804013681683 20.15319190821013 21 22.28746678119883 27.996402974257105 28.320985334514447 21.395144910029618 22 21.216805399274758 29.204313871993165 28.823796538433143 20.755084190298934 23 21.254225230807776 28.672735041396635 28.55260538189823 21.52043434589736 24 21.385908712653347 28.522821836663475 29.33440410891488 20.7568653417683 25 21.436975992984905 28.39391011014773 28.969255272506018 21.199858624361347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 190.0 2 253.0 3 316.0 4 316.0 5 316.0 6 495.5 7 675.0 8 675.0 9 675.0 10 1025.5 11 1376.0 12 1376.0 13 1376.0 14 2167.5 15 2959.0 16 2959.0 17 2959.0 18 4864.5 19 6770.0 20 6770.0 21 6770.0 22 10212.0 23 13654.0 24 13654.0 25 13654.0 26 18722.0 27 23790.0 28 23790.0 29 23790.0 30 29773.0 31 35756.0 32 35756.0 33 35756.0 34 41100.0 35 46444.0 36 46444.0 37 46444.0 38 50434.5 39 54425.0 40 54425.0 41 54425.0 42 56833.0 43 59241.0 44 59241.0 45 59241.0 46 60187.0 47 61133.0 48 61133.0 49 61133.0 50 57636.5 51 54140.0 52 54140.0 53 54140.0 54 46136.5 55 38133.0 56 38133.0 57 38133.0 58 31358.5 59 24584.0 60 24584.0 61 24584.0 62 18964.0 63 13344.0 64 13344.0 65 13344.0 66 9842.5 67 6341.0 68 6341.0 69 6341.0 70 4427.5 71 2514.0 72 2514.0 73 2514.0 74 1700.5 75 887.0 76 887.0 77 887.0 78 601.0 79 315.0 80 315.0 81 315.0 82 208.0 83 101.0 84 101.0 85 101.0 86 61.5 87 22.0 88 22.0 89 22.0 90 12.5 91 3.0 92 3.0 93 3.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21515717432875212 2 0.12323451461033516 3 0.1265893562058978 4 0.0713462979322993 5 0.02616776444538877 6 0.02616776444538877 7 0.3046196168770898 8 0.29835724589870616 9 0.022812922849826108 10 0.043612940742314615 11 0.019905393467005133 12 0.021694642317971885 13 0.019458081254263442 14 0.021247330105230198 15 0.019010769041521755 16 0.023260235062567795 17 0.023260235062567795 18 0.018339800722409223 19 0.02840432550909721 20 0.021023673998859356 21 0.017668832403296694 22 0.020576361786117665 23 0.02191829842434273 24 0.019458081254263442 25 0.017668832403296694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 447115.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.61156775466682 #Duplication Level Percentage of deduplicated Percentage of total 1 96.2494356090691 88.17561691648676 2 2.9933728791512677 5.4845516466669695 3 0.3642806595680384 1.001169669771962 4 0.11091587717837602 0.4064470958878039 5 0.07371155798170458 0.33764156941714907 6 0.03997174931929259 0.21971247726221588 7 0.02766557729002724 0.17741408358641117 8 0.021009753969858193 0.15397891994548354 9 0.01742608127586065 0.1436787562951655 >10 0.0860974974685855 1.6113634519120075 >50 0.008544644948897477 0.5421368027101069 >100 0.007079847438505899 1.387995275873465 >500 4.882653405865703E-4 0.35829333418453047 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 983 0.21985395256253984 No Hit GGTATCAACGCAGAGTACTTTTTTT 619 0.13844312984355256 No Hit TATCAACGCAGAGTACTTTTTTTTT 495 0.11070977265356786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 2.2365610637084418E-4 0.0 0.0 0.0 0.0 11 2.2365610637084418E-4 0.0 0.0 0.0 0.0 12 2.2365610637084418E-4 0.0 0.0 0.0 0.0 13 2.2365610637084418E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACC 30 7.608116E-4 19.037952 4 ATGACGA 25 0.0060850624 18.967669 17 TAGGACC 65 1.861572E-7 16.109037 4 GTAGGAC 215 0.0 14.185299 3 GGACTGG 70 7.1036193E-6 13.595485 6 GTTCTAG 65 5.2232306E-5 13.209785 1 TGTAGGA 225 0.0 13.131252 2 ACGTGGA 100 1.4151738E-7 12.362177 8 TAGGACG 140 5.0931703E-11 12.238684 4 GGACGTG 140 5.0931703E-11 12.235936 6 TCGAACT 70 1.1049771E-4 12.193502 19 TCTAAAC 55 0.0029892966 12.130042 3 GTCCTAC 185 0.0 11.861039 1 CTCGAAC 80 2.905873E-5 11.854793 18 GTGACAC 65 7.7896134E-4 11.73015 3 CCTACAC 90 7.164579E-6 11.648691 3 GGGACTG 75 2.03376E-4 11.420206 5 ATACAAT 100 1.8787796E-6 11.420206 6 GACGTGG 110 4.8254697E-7 11.247173 7 CCCTTAT 60 0.005682102 11.130467 1 >>END_MODULE