FastQCFastQC Report
Thu 26 May 2016
SRR1926997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926997_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447115
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16570.37059816825648884No Hit
GGTATCAACGCAGAGTACTTTTTTT11720.2621249566666294No Hit
TATCAACGCAGAGTACTTTTTTTTT9070.20285608847835568No Hit
CACACACACACACACACACACACAC5910.13218075886516892No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5750.12860226116323542No Hit
GTACATGGGAAGCAGTGGTATCAAC5180.11585386310009728No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4870.10892052380260112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC602.4743627E-514.301591
GGCCTCG400.00531766714.2312115
TTATAGT759.3496965E-713.9696464
CCTAGAC551.9106951E-413.855753
TAGGACC1101.7644197E-1013.8541944
TGTACTG903.4942786E-813.7579845
AGGACCT1259.640644E-1112.9536715
TGGACTG751.43408615E-512.6996785
CTACACT1155.0786184E-912.4235994
CCTTACA1001.3675162E-712.39471152
GAATAAG550.002980744512.1346841
ACTGAGT550.003095575412.0736168
GTGTATA1900.012.0434451
CCTGGAC951.0008716E-612.0326253
TACACTG2250.011.8530345
TGGCGAG1459.822543E-1111.77755418
GGACCTG1302.4865585E-911.722786
GCACTGT907.214956E-611.6413716
CTAAATT907.537139E-611.595819
GGACATG1406.4392225E-1011.5657796
Produced by FastQC (version 0.11.5)