##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926997_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 447115 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.961405902284646 31.0 30.0 33.0 26.0 34.0 2 30.26352951701464 31.0 30.0 34.0 26.0 34.0 3 30.30794985630095 31.0 30.0 34.0 26.0 34.0 4 33.889672679288324 35.0 35.0 37.0 30.0 37.0 5 33.85872762041085 37.0 35.0 37.0 30.0 37.0 6 33.81583708889212 37.0 35.0 37.0 30.0 37.0 7 33.81851201592431 37.0 35.0 37.0 29.0 37.0 8 33.84083289534013 37.0 35.0 37.0 30.0 37.0 9 35.37397537546269 39.0 35.0 39.0 30.0 39.0 10 35.26780358520738 39.0 35.0 39.0 30.0 39.0 11 35.29731053532089 39.0 35.0 39.0 30.0 39.0 12 35.1933193921027 39.0 35.0 39.0 29.0 39.0 13 35.220211802332734 39.0 35.0 39.0 29.0 39.0 14 36.30593471478255 40.0 36.0 41.0 29.0 41.0 15 36.29100119656017 40.0 36.0 41.0 29.0 41.0 16 36.269342339219214 40.0 36.0 41.0 29.0 41.0 17 36.25860908267448 40.0 36.0 41.0 29.0 41.0 18 36.23871710857386 40.0 36.0 41.0 29.0 41.0 19 36.325442000380214 40.0 37.0 41.0 29.0 41.0 20 36.27882535812934 40.0 37.0 41.0 29.0 41.0 21 36.23884011943236 40.0 37.0 41.0 29.0 41.0 22 36.151140087002226 40.0 37.0 41.0 29.0 41.0 23 36.08571396620556 40.0 37.0 41.0 29.0 41.0 24 36.01467855026112 40.0 36.0 41.0 29.0 41.0 25 35.90577815550809 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14021352900437734 1101 2 -0.01900174502092966 1101 3 0.16780071024206222 1101 4 0.21238017363867812 1101 5 -0.1631136920499472 1101 6 -0.2248588705288057 1101 7 -0.16153103418196935 1101 8 0.08447380909372981 1101 9 -0.02426330798570575 1101 10 -0.1796540872804684 1101 11 -0.06221013934837316 1101 12 0.14791795707817812 1101 13 0.028328733328336853 1101 14 0.03664529931398164 1101 15 -0.2543030313999637 1101 16 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25 -0.03991231114502369 2213 1 0.5527037994695476 2213 2 0.5092846153880153 2213 3 0.45407082610450544 2213 4 0.5358346228303503 2213 5 0.3237404558911692 2213 6 0.45761729414936525 2213 7 0.3073917282938652 2213 8 0.41604642730584374 2213 9 0.5265327076267852 2213 10 0.782905064949567 2213 11 0.5202951653702641 2213 12 0.3878882424453636 2213 13 0.39913985251109807 2213 14 0.31740548498939347 2213 15 0.48989666406609444 2213 16 0.35014897607712925 2213 17 0.2713248396185719 2213 18 0.38619643752922883 2213 19 0.390890445269072 2213 20 0.4960794721964845 2213 21 0.2715771820450996 2213 22 0.3564845879830756 2213 23 0.5332769664447881 2213 24 0.2801933745180989 2213 25 0.1081243014609683 2214 1 0.0451151477762366 2214 2 0.08406426081832308 2214 3 0.16479357426064567 2214 4 -0.1789930055723019 2214 5 -0.049673862613424546 2214 6 -0.2487966275872182 2214 7 -0.2129824943931098 2214 8 -0.20497893988997617 2214 9 -0.24685899139316092 2214 10 -0.43281436444612353 2214 11 -0.19128230265653912 2214 12 -0.39112310066996514 2214 13 -0.32542342457016815 2214 14 -0.3840951880329797 2214 15 -0.36457661713118483 2214 16 -0.17440799721333633 2214 17 -0.3869763258411041 2214 18 -0.5943378272818975 2214 19 -0.38019295388325247 2214 20 -0.4648889383953687 2214 21 -0.44155583906518103 2214 22 -0.5340612090571071 2214 23 -0.2089925465688296 2214 24 -0.3001113581455499 2214 25 -0.36600976162885956 2215 1 0.47499526982601736 2215 2 0.47732944850368497 2215 3 0.3351826168392158 2215 4 0.6385055435733804 2215 5 0.3512817430247921 2215 6 0.3402003781343055 2215 7 0.3027739614635365 2215 8 0.1603995817695889 2215 9 0.28665129276066637 2215 10 0.07796824239193967 2215 11 -0.15192053613905188 2215 12 -0.14431765044228229 2215 13 -0.12575916827987044 2215 14 -0.25667991614466246 2215 15 -0.09622909568879834 2215 16 0.12267712075981052 2215 17 -0.02931492497450705 2215 18 0.06415955049920541 2215 19 -0.10416391543472514 2215 20 0.11502554130869669 2215 21 0.1995223860148414 2215 22 0.02751554024594327 2215 23 0.12314984009541519 2215 24 0.04564057603017346 2215 25 0.05297560165438142 2216 1 -0.2697802945830112 2216 2 -0.24612340674200084 2216 3 -1.8740232264542769 2216 4 -1.832458599852913 2216 5 -0.7063065703882216 2216 6 -0.2002139644951768 2216 7 -0.5778475524806836 2216 8 -0.28202272898737846 2216 9 -0.42249080551290774 2216 10 -0.5950091812468372 2216 11 -0.4696522758468902 2216 12 -0.525146335701244 2216 13 -0.5811589026756181 2216 14 -0.8001380834753391 2216 15 -0.8085265724484287 2216 16 -0.7247279257209343 2216 17 -0.585399918334403 2216 18 -0.6329294269244343 2216 19 -0.7509740083962129 2216 20 -0.6577075264203884 2216 21 -0.6649981983145139 2216 22 -0.8073051768855279 2216 23 -0.9022472382578357 2216 24 -0.8164152008622594 2216 25 -0.7614413970175988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10088.0 3 11.0 4 156.0 5 1097.0 6 1520.0 7 1211.0 8 946.0 9 805.0 10 676.0 11 643.0 12 574.0 13 560.0 14 535.0 15 546.0 16 547.0 17 563.0 18 659.0 19 804.0 20 1024.0 21 1368.0 22 1723.0 23 2054.0 24 2508.0 25 3015.0 26 3472.0 27 3889.0 28 4367.0 29 5453.0 30 7076.0 31 9165.0 32 11922.0 33 15975.0 34 21597.0 35 29876.0 36 43938.0 37 76804.0 38 179948.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.02178157522346 19.75612158991169 10.759354792483924 27.462742042380928 2 13.836099194870096 21.098388844607243 41.00050152697051 24.065010433552153 3 18.741569716071393 24.891007473453694 29.041559202682482 27.325863607792435 4 11.269136575601356 17.822931460586204 38.77906131532156 32.12887064849088 5 12.346899519561969 39.14764746405662 35.47636728011237 13.029085736269044 6 30.685159002670616 38.68960254942885 18.240535020983415 12.38470342691712 7 27.901941002646314 32.433556953898666 21.31725114475983 18.347250898695187 8 24.55365331019026 38.4790884730492 19.681752908914408 17.285505307846126 9 25.836662386987392 16.46819094825465 20.19084088987505 37.50430577488291 10 13.763394684398758 31.1642387927506 34.51070120935184 20.5616653134988 11 33.85716170908513 23.949603326377286 23.367397834455023 18.825837130082558 12 21.93266146888713 26.709071959276603 31.9412946139016 19.416971957934663 13 28.036411214117173 22.800845420082084 25.904073895977547 23.2586694698232 14 19.88302785636805 23.330015767755498 28.75613656441855 28.030819811457903 15 22.648594849084667 31.040806969280148 23.90870153543351 22.401896646201674 16 21.061518986209336 30.39045970378919 27.83764319614237 20.710378113859104 17 20.661574762644957 29.681401876474734 28.08829943079521 21.568723930085103 18 20.108070872305497 29.585519576662776 29.71054361974798 20.595865931283743 19 21.638951947485545 28.534269706898673 28.924549612515793 20.902228733099985 20 21.704979043375964 28.458066622829968 29.65597140773941 20.180982926054654 21 22.17703782739114 27.8279950878747 28.67486696327314 21.32010012146102 22 21.217583843082878 28.935508761727966 28.84112588483947 21.005781510349685 23 21.132194473892564 28.524492634987947 28.747254615154077 21.59605827596541 24 20.855931919081222 28.360712568354895 29.830804155530455 20.952551357033425 25 21.22803564398955 28.270497083348246 29.106574097269437 21.394893175392767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 230.0 3 246.0 4 246.0 5 246.0 6 396.0 7 546.0 8 546.0 9 546.0 10 902.0 11 1258.0 12 1258.0 13 1258.0 14 1963.0 15 2668.0 16 2668.0 17 2668.0 18 4514.5 19 6361.0 20 6361.0 21 6361.0 22 9663.5 23 12966.0 24 12966.0 25 12966.0 26 17823.0 27 22680.0 28 22680.0 29 22680.0 30 28789.5 31 34899.0 32 34899.0 33 34899.0 34 40318.0 35 45737.0 36 45737.0 37 45737.0 38 49524.0 39 53311.0 40 53311.0 41 53311.0 42 56212.0 43 59113.0 44 59113.0 45 59113.0 46 60827.5 47 62542.0 48 62542.0 49 62542.0 50 59055.5 51 55569.0 52 55569.0 53 55569.0 54 47585.5 55 39602.0 56 39602.0 57 39602.0 58 32305.5 59 25009.0 60 25009.0 61 25009.0 62 19399.5 63 13790.0 64 13790.0 65 13790.0 66 10171.0 67 6552.0 68 6552.0 69 6552.0 70 4619.5 71 2687.0 72 2687.0 73 2687.0 74 1814.5 75 942.0 76 942.0 77 942.0 78 625.5 79 309.0 80 309.0 81 309.0 82 198.0 83 87.0 84 87.0 85 87.0 86 54.0 87 21.0 88 21.0 89 21.0 90 13.5 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11160439707905126 2 0.10713127495163435 3 0.014761303020475718 4 0.0 5 0.005144090446529416 6 0.34107556221553736 7 0.017445176296925845 8 0.009169900361204611 9 0.009169900361204611 10 0.0035784977019335073 11 0.004920434340158573 12 2.236561063708442E-4 13 0.0 14 0.0 15 0.002236561063708442 16 2.236561063708442E-4 17 0.0 18 2.236561063708442E-4 19 0.0 20 2.236561063708442E-4 21 0.012972054169508963 22 0.0 23 2.236561063708442E-4 24 0.0 25 0.006038714872012793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 447115.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.41785646678294 #Duplication Level Percentage of deduplicated Percentage of total 1 95.17182940022538 85.10060980990504 2 3.911602177926443 6.9953416420196435 3 0.4349099934028302 1.1666615809819134 4 0.16078295397944983 0.5750746840495924 5 0.07493389326376362 0.3350214056178224 6 0.05590748379149994 0.29994764166524013 7 0.02819381989209685 0.17647216582532918 8 0.024534178525409567 0.17550349231324008 9 0.015875545578001384 0.12776015302430385 >10 0.10115548911185958 1.7822781596618227 >50 0.010262687517517988 0.6242377174810637 >100 0.008510520267927306 1.4279800061663273 >500 0.001001237678579647 0.5799210338521755 >1k 5.006188392898235E-4 0.6331905074364355 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1657 0.37059816825648884 No Hit GGTATCAACGCAGAGTACTTTTTTT 1172 0.2621249566666294 No Hit TATCAACGCAGAGTACTTTTTTTTT 907 0.20285608847835568 No Hit CACACACACACACACACACACACAC 591 0.13218075886516892 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 575 0.12860226116323542 No Hit GTACATGGGAAGCAGTGGTATCAAC 518 0.11585386310009728 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 487 0.10892052380260112 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTTAC 60 2.4743627E-5 14.30159 1 GGCCTCG 40 0.005317667 14.23121 15 TTATAGT 75 9.3496965E-7 13.969646 4 CCTAGAC 55 1.9106951E-4 13.85575 3 TAGGACC 110 1.7644197E-10 13.854194 4 TGTACTG 90 3.4942786E-8 13.757984 5 AGGACCT 125 9.640644E-11 12.953671 5 TGGACTG 75 1.43408615E-5 12.699678 5 CTACACT 115 5.0786184E-9 12.423599 4 CCTTACA 100 1.3675162E-7 12.3947115 2 GAATAAG 55 0.0029807445 12.134684 1 ACTGAGT 55 0.0030955754 12.073616 8 GTGTATA 190 0.0 12.043445 1 CCTGGAC 95 1.0008716E-6 12.032625 3 TACACTG 225 0.0 11.853034 5 TGGCGAG 145 9.822543E-11 11.777554 18 GGACCTG 130 2.4865585E-9 11.72278 6 GCACTGT 90 7.214956E-6 11.641371 6 CTAAATT 90 7.537139E-6 11.5958 19 GGACATG 140 6.4392225E-10 11.565779 6 >>END_MODULE