FastQCFastQC Report
Thu 26 May 2016
SRR1926996_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926996_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481306
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT6780.14086672511873943No Hit
GTCCTACAGTGGACATTTCTAAATT4900.10180633526280579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAC601.4090074E-615.8891323
AGGACCT1600.013.681515
CCCCTAT500.001448580813.3580341
GTCCTAC2750.012.8375921
CTAGGAC603.9634467E-412.7113053
GGACTGA701.0692631E-412.2368786
GAAGTGC550.00302102412.1132746
GTCCAGG959.817559E-712.05236151
TAGGACC1209.595169E-911.9031724
TCCTACA3100.011.68622
TGTAGGA2450.011.6736492
CCTACAG2750.011.4401763
GGACCTG2000.011.4210866
AGGACGT1803.6379788E-1211.1038345
GTGTAGA951.29080745E-511.0479981
GACCTGG1900.011.0203477
GATATAC1053.2739208E-610.9045181
TTATACT803.6789777E-410.7128544
GACGTGA803.6967619E-410.7072697
GTAGGAC2500.010.6774973
Produced by FastQC (version 0.11.5)