##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 481306 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.861067595251253 31.0 28.0 31.0 16.0 34.0 2 27.998917528557715 31.0 28.0 31.0 16.0 34.0 3 27.776360568951976 31.0 28.0 31.0 16.0 34.0 4 31.348825902855978 35.0 32.0 37.0 17.0 37.0 5 31.111243159237574 35.0 32.0 37.0 16.0 37.0 6 30.88389299115324 35.0 32.0 37.0 11.0 37.0 7 30.61863346810553 35.0 31.0 37.0 11.0 37.0 8 30.436422982468535 35.0 30.0 37.0 10.0 37.0 9 32.65056325913245 37.0 32.0 39.0 17.0 39.0 10 32.92322763481029 37.0 33.0 39.0 17.0 39.0 11 33.03815244355982 37.0 33.0 39.0 17.0 39.0 12 32.97675075731448 37.0 33.0 39.0 15.0 39.0 13 33.03793636480742 38.0 33.0 39.0 16.0 39.0 14 34.03081823206027 39.0 34.0 40.0 16.0 41.0 15 33.621114218397445 39.0 33.0 40.0 10.0 41.0 16 33.689839727740775 39.0 33.0 40.0 10.0 41.0 17 33.90728143841963 39.0 33.0 40.0 11.0 41.0 18 33.9517022434792 39.0 34.0 41.0 10.0 41.0 19 33.32353222274395 39.0 33.0 40.0 8.0 41.0 20 33.64326229051788 39.0 33.0 40.0 8.0 41.0 21 33.60323162395648 39.0 33.0 40.0 2.0 41.0 22 33.60797912346823 39.0 34.0 40.0 2.0 41.0 23 33.58356222444765 39.0 34.0 40.0 2.0 41.0 24 32.292452202964434 38.0 32.0 40.0 2.0 41.0 25 32.8549446713733 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.23467169980894909 1101 2 -0.2696289266751961 1101 3 -0.9623275830685003 1101 4 -0.4684909054207722 1101 5 -0.7384780703517713 1101 6 -2.0583052917176907 1101 7 -0.8959452929278662 1101 8 -0.38345963938134275 1101 9 -0.4158627520071505 1101 10 -9.182529430331694 1101 11 -4.171713998386309 1101 12 -1.591235955030296 1101 13 -0.8187939036374559 1101 14 -0.5358822253077875 1101 15 -2.414927442899451 1101 16 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0.03585808631181564 1103 3 0.45094352082760025 1103 4 0.29655779587793063 1103 5 -0.3808806677543686 1103 6 0.603521006983609 1103 7 0.744111525253949 1103 8 0.4041215294498244 1103 9 0.41989211812271776 1103 10 0.2517319333046686 1103 11 -0.05527731007462222 1103 12 0.16819586315151724 1103 13 0.14528077168721865 1103 14 0.0941908266402649 1103 15 0.393489764892756 1103 16 0.3448799692043991 1103 17 0.2712534033223051 1103 18 -0.01990609411019051 1103 19 -1.893858971111591 1103 20 -0.3015695951002755 1103 21 -0.044397492302813646 1103 22 -0.07120317431289891 1103 23 -0.11558238860582293 1103 24 1.4742227851520084 1103 25 0.6469880515555673 1104 1 -0.20177675925903316 1104 2 -0.10519679885750932 1104 3 -1.5231903834152938 1104 4 -0.5536485100776964 1104 5 -0.39636139955662486 1104 6 -1.5809048396493246 1104 7 -0.014199101449047902 1104 8 0.18561238192160445 1104 9 0.11041372185957954 1104 10 0.2243204891629773 1104 11 0.043146201019190755 1104 12 0.07086616287904235 1104 13 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2216 14 -2.17745419500476 2216 15 -3.386676180273188 2216 16 -2.3965413872169528 2216 17 -2.5600597279908293 2216 18 -2.331781995986095 2216 19 -1.6291475714001926 2216 20 -2.301620100150778 2216 21 -4.826316684222007 2216 22 -3.6254888885986105 2216 23 -3.3577108157342472 2216 24 -3.64990419897498 2216 25 -2.931886407318892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30987.0 3 2.0 4 222.0 5 1476.0 6 1811.0 7 1453.0 8 1112.0 9 1005.0 10 996.0 11 967.0 12 877.0 13 918.0 14 1009.0 15 994.0 16 1094.0 17 1114.0 18 1304.0 19 1609.0 20 1992.0 21 2689.0 22 3252.0 23 3936.0 24 4551.0 25 5381.0 26 6043.0 27 6585.0 28 7309.0 29 8627.0 30 10338.0 31 12380.0 32 16132.0 33 21296.0 34 28196.0 35 39308.0 36 56904.0 37 88225.0 38 109212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30962966047872 18.95905129780212 12.096577717159306 25.63474132455985 2 14.080738945463246 20.742482082860917 41.69487294173939 23.481906029936443 3 18.574905860569622 25.141781263652817 30.05305094971602 26.230261926061537 4 11.225368101093181 17.53288064834027 39.70252525042517 31.53922600014138 5 12.061254940752532 39.32070107771788 36.20745549685995 12.41058848466964 6 30.956933026325633 38.0699246057805 18.91547036184238 12.057672006051487 7 26.945968024273544 32.62257639664571 22.96879949318974 17.462656085891002 8 24.035842667361294 37.39515498827819 21.444334462099505 17.124667882261004 9 25.92868126844271 15.855533851460871 20.64003989859108 37.57574498150534 10 13.340954202043084 30.422022924958693 35.86935059806499 20.36767227493323 11 33.763062799101476 23.59127491256834 24.38673158324554 18.258930705084648 12 21.919729689292307 26.015743188203416 32.63698402407195 19.42754309843233 13 28.036348070385426 22.802117879792867 26.106317898079112 23.0552161517426 14 19.928183729957524 22.817666469947717 28.94573881819013 28.308410981904625 15 22.757696343782143 31.396718858769624 23.800430143277193 22.04515465417104 16 21.25846806034662 29.60558580275134 28.207472673621215 20.928473463280827 17 20.923771307688952 29.33962009297145 28.256961949034025 21.479646650305575 18 20.549041120939908 29.16056263104196 29.782706721608182 20.507689526409955 19 22.608317972398115 28.076118196421856 28.240499252907842 21.07506457827219 20 22.264659860482226 28.411005986754745 29.029214781320327 20.295119371442702 21 22.35214176769083 27.893474746383284 27.892851354215587 21.861532131710298 22 21.683900354925317 28.990416185259377 28.318385463854973 21.007297995960332 23 21.371871012788173 28.532747607153397 28.32993229792239 21.765449082136044 24 21.86746074130719 28.299714690034516 28.81547531227207 21.017349256386225 25 21.80164118036984 28.261632993169716 28.46839096581948 21.46833486064097 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 171.0 1 171.0 2 222.0 3 273.0 4 273.0 5 273.0 6 391.5 7 510.0 8 510.0 9 510.0 10 845.0 11 1180.0 12 1180.0 13 1180.0 14 1970.5 15 2761.0 16 2761.0 17 2761.0 18 4664.5 19 6568.0 20 6568.0 21 6568.0 22 10241.5 23 13915.0 24 13915.0 25 13915.0 26 19533.5 27 25152.0 28 25152.0 29 25152.0 30 32109.5 31 39067.0 32 39067.0 33 39067.0 34 44834.5 35 50602.0 36 50602.0 37 50602.0 38 54984.0 39 59366.0 40 59366.0 41 59366.0 42 61523.5 43 63681.0 44 63681.0 45 63681.0 46 64455.5 47 65230.0 48 65230.0 49 65230.0 50 60944.0 51 56658.0 52 56658.0 53 56658.0 54 48931.0 55 41204.0 56 41204.0 57 41204.0 58 34186.5 59 27169.0 60 27169.0 61 27169.0 62 21203.5 63 15238.0 64 15238.0 65 15238.0 66 11435.0 67 7632.0 68 7632.0 69 7632.0 70 5417.0 71 3202.0 72 3202.0 73 3202.0 74 2192.5 75 1183.0 76 1183.0 77 1183.0 78 786.0 79 389.0 80 389.0 81 389.0 82 246.5 83 104.0 84 104.0 85 104.0 86 70.5 87 37.0 88 37.0 89 37.0 90 23.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22251956136013265 2 0.1290239473432702 3 0.13214046781049893 4 0.06731684209214096 5 0.021607875239452658 6 0.02119233917715549 7 0.29960150091625704 8 0.2973160525736226 9 0.01786805067877816 10 0.03552833332640773 11 0.015167066273846577 12 0.017036978554183825 13 0.014543762180400826 14 0.01620590642958949 15 0.014751530211549409 16 0.01786805067877816 17 0.01724474658533241 18 0.013920458086955075 19 0.022231179332898407 20 0.015998138398440907 21 0.01412822611810366 22 0.01620590642958949 23 0.01662144249188666 24 0.015582602336143742 25 0.013920458086955075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 481306.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.42544328260507 #Duplication Level Percentage of deduplicated Percentage of total 1 95.76122005876073 87.5501199315529 2 3.400400748659814 6.217662915694513 3 0.41707892009709574 1.143948754611216 4 0.14747982709361354 0.5393363426910227 5 0.06620892921244576 0.3026590351257237 6 0.03921391700209527 0.21510898468583062 7 0.030186967714989944 0.193189983329045 8 0.01797886955303306 0.1314980894884943 9 0.015065483209036262 0.12396316325875054 >10 0.08992817133101208 1.5564144865912293 >50 0.009097378933084571 0.6038373046641932 >100 0.0059132940466320935 1.2812825048521888 >500 2.2743438640874974E-4 0.14097850345496263 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 678 0.14086672511873943 No Hit GTCCTACAGTGGACATTTCTAAATT 490 0.10180633526280579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.233040934457497E-4 0.0 10 0.0 0.0 0.0 6.233040934457497E-4 0.0 11 0.0 0.0 0.0 6.233040934457497E-4 0.0 12 0.0 0.0 0.0 6.233040934457497E-4 0.0 13 0.0 0.0 0.0 6.233040934457497E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCAC 60 1.4090074E-6 15.889132 3 AGGACCT 160 0.0 13.68151 5 CCCCTAT 50 0.0014485808 13.358034 1 GTCCTAC 275 0.0 12.837592 1 CTAGGAC 60 3.9634467E-4 12.711305 3 GGACTGA 70 1.0692631E-4 12.236878 6 GAAGTGC 55 0.003021024 12.113274 6 GTCCAGG 95 9.817559E-7 12.0523615 1 TAGGACC 120 9.595169E-9 11.903172 4 TCCTACA 310 0.0 11.6862 2 TGTAGGA 245 0.0 11.673649 2 CCTACAG 275 0.0 11.440176 3 GGACCTG 200 0.0 11.421086 6 AGGACGT 180 3.6379788E-12 11.103834 5 GTGTAGA 95 1.29080745E-5 11.047998 1 GACCTGG 190 0.0 11.020347 7 GATATAC 105 3.2739208E-6 10.904518 1 TTATACT 80 3.6789777E-4 10.712854 4 GACGTGA 80 3.6967619E-4 10.707269 7 GTAGGAC 250 0.0 10.677497 3 >>END_MODULE