FastQCFastQC Report
Thu 26 May 2016
SRR1926996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926996_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481306
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10820.22480500970276707No Hit
GGTATCAACGCAGAGTACTTTTTTT6840.14211333330563092No Hit
GTACATGGGAAGCAGTGGTATCAAC5160.10720830407266896No Hit
TATCAACGCAGAGTACTTTTTTTTT5050.10492285573003453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1500.013.96322254
CTACACT1101.7826096E-1013.8478254
GTATAGA801.9212148E-613.1041811
CCTTAGT603.9745806E-412.7070862
CCTACAC751.439888E-512.6951633
TGTAGGA3150.012.4045362
TCTACAC853.829842E-612.3217763
CTAGGAC701.0652868E-412.2417653
TAAGACT951.0083531E-612.0257434
CCCTACA657.7945157E-411.7296182
GTAGGAC3100.011.671363
CTATAGG752.0078097E-411.4363781
CTGCATC752.0890628E-411.3876569
TGGCGAG2700.011.24589818
TATACTG855.1903153E-511.2004465
TCTAGGA600.00572814311.11872
CCCTTAG600.00572814311.11871
CTATCCC600.00577280711.107114
CAGCATA600.00577280711.107114
CTGTAGG3450.011.049641
Produced by FastQC (version 0.11.5)