FastQCFastQC Report
Thu 26 May 2016
SRR1926995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581168
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9850.16948627591333315No Hit
GTCCTACAGTGGACATTTCTAAATT7570.13025493488973927No Hit
GTACATGGGAAGCAGTGGTATCAAC6970.11993089777826721No Hit
CTGTAGGACGTGGAATATGGCAAGA6800.11700575393001679No Hit
GTCCTAAAGTGTGTATTTCTCATTT6220.10702585138892713No Hit
CCCATGTACTCTGCGTTGATACCAC5930.1020359001183823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAC1051.2951205E-913.6178953
GGGTAGG500.001449041813.3582471
CCTATAC500.001459479613.3455373
TAGGACC2100.013.14917854
GTGTAGG905.107304E-712.722141
TGTTACA701.0535764E-412.2571652
GTCCTAC4000.011.4499261
TGAACTG752.0356805E-411.4202615
GGCGAGA1751.8189894E-1211.37898619
GTGTTAG1104.6751848E-711.2764431
GTCTTAA600.00568025911.1318731
AGGACCT2950.010.9686135
CTATGAC700.001447089410.8943163
TCTACAC1053.3147626E-610.8943163
CTACACT1053.3597298E-610.8820784
TAACACT700.001460728210.8820774
GTCCTAA1951.8189894E-1210.7648891
CTTATAG803.6459567E-410.7240913
CTAAGAG909.167188E-510.5916963
TGGCGAG3100.010.40014818
Produced by FastQC (version 0.11.5)