##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926995_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 581168 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.153829873633786 31.0 28.0 31.0 16.0 34.0 2 28.281906436693003 31.0 28.0 31.0 16.0 34.0 3 28.047788247116152 31.0 28.0 31.0 16.0 34.0 4 31.64291908707981 35.0 32.0 37.0 19.0 37.0 5 31.383467431104258 35.0 32.0 37.0 17.0 37.0 6 31.172261721223467 35.0 32.0 37.0 16.0 37.0 7 30.91343466949316 35.0 32.0 37.0 11.0 37.0 8 30.749086322715634 35.0 31.0 37.0 11.0 37.0 9 32.967708132588164 37.0 33.0 39.0 17.0 39.0 10 33.211694380970734 37.0 33.0 39.0 17.0 39.0 11 33.34379903917628 38.0 34.0 39.0 17.0 39.0 12 33.28450981471795 38.0 34.0 39.0 17.0 39.0 13 33.35430718828291 38.0 34.0 39.0 17.0 39.0 14 34.374476915453016 39.0 34.0 41.0 17.0 41.0 15 33.97409182886876 39.0 33.0 41.0 11.0 41.0 16 34.03257749910525 39.0 33.0 40.0 17.0 41.0 17 34.26320100211987 39.0 34.0 41.0 17.0 41.0 18 34.301296699061204 39.0 34.0 41.0 17.0 41.0 19 33.684294386476886 39.0 33.0 40.0 10.0 41.0 20 33.97846061723977 39.0 34.0 41.0 10.0 41.0 21 33.96250997990254 39.0 34.0 41.0 10.0 41.0 22 33.975402981581915 39.0 34.0 41.0 8.0 41.0 23 33.95801042039479 39.0 34.0 41.0 2.0 41.0 24 32.666266897007404 39.0 32.0 40.0 2.0 41.0 25 33.24025066762107 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.09016530273227374 1101 2 -0.047947772497884955 1101 3 -0.6974755216841615 1101 4 -0.09789977159358898 1101 5 -0.08272020360553611 1101 6 -1.5823296109109393 1101 7 -0.3038904524475292 1101 8 0.32466337937467316 1101 9 0.33303199510925907 1101 10 -9.61040790113519 1101 11 -3.6880797374775973 1101 12 -1.147595522553253 1101 13 -0.3892347991089693 1101 14 -0.09267103166668278 1101 15 -1.7314109707410736 1101 16 -0.11895191634403801 1101 17 -0.025722482500960098 1101 18 0.3254786806893861 1101 19 -0.5826524930500767 1101 20 0.17337964113112747 1101 21 0.5032437513693111 1101 22 0.685513478358402 1101 23 0.5105520911352173 1101 24 0.33763358569776614 1101 25 0.4891411393890124 1102 1 -0.1717425104460908 1102 2 -0.31880017629123003 1102 3 -0.20390411926650742 1102 4 -0.3685298683102438 1102 5 -0.10716352053173495 1102 6 -0.7914146472908357 1102 7 0.08707054181433094 1102 8 0.18237115803005466 1102 9 0.10314032404004081 1102 10 0.3816701208025961 1102 11 0.1645672859614109 1102 12 0.1696432143240969 1102 13 0.038164940399440184 1102 14 -0.08523028304106361 1102 15 0.4717894665500211 1102 16 0.2596265737462957 1102 17 -0.08685823627887146 1102 18 -0.09166485645158673 1102 19 0.13818343386581944 1102 20 0.3069064252108049 1102 21 0.06447365873264488 1102 22 0.22001684434908242 1102 23 -0.27900679931708794 1102 24 -3.5981052845638253 1102 25 -1.9046117702183167 1103 1 0.004335809644715027 1103 2 -0.3367166390264451 1103 3 0.18752320997778327 1103 4 -0.15062564146210633 1103 5 -0.5908481366372875 1103 6 0.5848531988806336 1103 7 0.10513800060282108 1103 8 0.1387567290554763 1103 9 0.05540864923347044 1103 10 0.20708353758294606 1103 11 -0.1954255287091513 1103 12 -0.09881735487896037 1103 13 -0.22964087867604377 1103 14 -0.13058377000854193 1103 15 0.17120709039281934 1103 16 0.3368623566868649 1103 17 0.04551583821945826 1103 18 -0.23106805756791715 1103 19 -1.7504166244498975 1103 20 -0.5530187015738122 1103 21 -0.3232581722767378 1103 22 -0.1694097871893021 1103 23 -0.16114917297504405 1103 24 1.197942003234651 1103 25 0.4126075072916038 1104 1 -0.7247365885453299 1104 2 -0.5832710829635133 1104 3 -1.8774805261673464 1104 4 -0.9824108961426639 1104 5 -0.9711254416312656 1104 6 -2.040323230194879 1104 7 -0.8155519408642355 1104 8 -0.2561538110250581 1104 9 -0.4637442836404517 1104 10 -0.47759100266364385 1104 11 -0.34715453364916726 1104 12 -0.3646337025895292 1104 13 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0.2070735655047642 1107 11 0.031189947956185904 1107 12 0.4480015521812746 1107 13 0.31745724657325525 1107 14 0.4706128793731921 1107 15 -0.9834997305086546 1107 16 -0.054701265171928526 1107 17 0.11618795628886147 1107 18 0.02981147972765541 1107 19 1.0262886009190666 1107 20 0.9457148444971892 1107 21 0.6725635269653836 1107 22 0.36550245852269825 1107 23 0.2938069498809597 1107 24 -3.67817486952163 1107 25 -1.6256254404547086 1108 1 0.40798445829336316 1108 2 0.0639528377898202 1108 3 0.49468041305241783 1108 4 0.5408335700429774 1108 5 0.8421471522295612 1108 6 -2.994855454727105 1108 7 -1.1700120117948956 1108 8 0.3153919881678 1108 9 0.09573718878219495 1108 10 0.40191492860699896 1108 11 0.19702426052962352 1108 12 0.4809098953070077 1108 13 0.42629390932296474 1108 14 0.32018984764580694 1108 15 -0.8925655354440281 1108 16 0.25801906385274265 1108 17 0.14342062373891906 1108 18 0.4066681189756025 1108 19 0.4993614563832267 1108 20 -1.0947023117895327 1108 21 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1 0.9538373836216287 2215 2 0.4356439412752593 2215 3 0.7220171306724517 2215 4 0.9944293273952951 2215 5 1.186838454234607 2215 6 1.4941630075967112 2215 7 1.1636302558662592 2215 8 0.025240262465182184 2215 9 0.6624017757196015 2215 10 0.4697246900998664 2215 11 0.26457764812498397 2215 12 0.2701478636005916 2215 13 0.34688833375788874 2215 14 0.6087738356211929 2215 15 0.750763922030238 2215 16 0.9786225634698837 2215 17 0.5485651176557127 2215 18 -0.21498413138598949 2215 19 0.9817028533940899 2215 20 -3.2787833077907784 2215 21 -1.378530272934249 2215 22 -0.49179828512436075 2215 23 -0.38307269791319243 2215 24 -0.3947746231600249 2215 25 -0.15426725805587438 2216 1 -0.7428614997723244 2216 2 -1.2002333214636032 2216 3 -0.4527295978553525 2216 4 -0.7042470262483818 2216 5 -0.7167172278455816 2216 6 -2.240737166034826 2216 7 -9.727615938591647 2216 8 -11.457470695143314 2216 9 -6.260606284736166 2216 10 -3.8636419735866774 2216 11 -2.7322873380644843 2216 12 -2.435479633234987 2216 13 -2.2479843599777283 2216 14 -2.100921532792924 2216 15 -3.0895974103682775 2216 16 -2.111305866458732 2216 17 -2.4240196865265027 2216 18 -2.110454654021609 2216 19 -1.6339326897808704 2216 20 -2.6388581716853032 2216 21 -5.023951388222763 2216 22 -3.370493837051484 2216 23 -3.4147181037357335 2216 24 -3.5770147993325097 2216 25 -3.141378998653188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34345.0 3 3.0 4 262.0 5 1606.0 6 2221.0 7 1685.0 8 1337.0 9 1147.0 10 1059.0 11 1079.0 12 1047.0 13 1015.0 14 1033.0 15 1098.0 16 1204.0 17 1247.0 18 1546.0 19 1950.0 20 2391.0 21 2902.0 22 3869.0 23 4506.0 24 5284.0 25 6172.0 26 6806.0 27 7670.0 28 8601.0 29 9984.0 30 11688.0 31 14737.0 32 19195.0 33 25255.0 34 33668.0 35 47145.0 36 68747.0 37 107985.0 38 139679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92656177791267 19.23347519295898 12.568371971113057 25.271591058015296 2 14.22455304918914 20.49401050594987 41.89854815284452 23.382888292016478 3 18.32685247240394 25.292414033117 30.03823047515019 26.342503019328873 4 11.374257425742575 17.52647438656909 39.29246663796814 31.806801549720188 5 12.24214073530981 39.177913857020414 35.82957279608422 12.750372611585552 6 30.998198049349696 37.84320103539017 18.94388825496483 12.2147126602953 7 27.08570915922936 32.640009109369366 22.799647356988938 17.474634374412336 8 24.32072460642657 37.22993314643088 21.19525555315937 17.25408669398318 9 26.185809014230916 15.986672609838243 20.623206937677587 37.20431143825326 10 13.966686231950165 29.97843228719312 35.11165121238775 20.94323026846897 11 33.19841191989385 23.679740620466408 24.696469843171066 18.425377616468673 12 22.513449418826777 25.8564431579201 32.014607634933036 19.615499788320086 13 27.968093894128177 22.842809940111515 26.098988091140633 23.09010807461967 14 20.328980995306907 22.88359173726954 28.465220017657174 28.32220724976638 15 22.754619829903525 31.226918059757487 23.744669427034395 22.273792683304595 16 21.289404346202364 29.29995060742541 28.20642998387427 21.204215062497955 17 21.052867082801978 29.26181588507919 28.21889612278229 21.46642090933654 18 20.894261232904764 28.630161267839533 29.723636726595 20.751940772660703 19 22.88883534413025 27.870161609555446 28.04123711340206 21.199765932912243 20 22.76038556389839 28.181237748915365 28.61491969139744 20.44345699578881 21 22.35345340900877 27.594746316329307 27.78542639714462 22.266373877517303 22 22.202909125954275 28.54399140892288 28.11650271568904 21.1365967494338 23 21.57566662421566 28.401272153401873 28.178920665953033 21.844140556429434 24 22.274557720107385 27.92747986507882 28.60019274454464 21.197769670269153 25 21.900674264040283 27.985743738362146 28.459174155621103 21.654407841976465 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 235.0 1 235.0 2 290.5 3 346.0 4 346.0 5 346.0 6 555.0 7 764.0 8 764.0 9 764.0 10 1083.5 11 1403.0 12 1403.0 13 1403.0 14 2362.5 15 3322.0 16 3322.0 17 3322.0 18 5716.5 19 8111.0 20 8111.0 21 8111.0 22 12512.0 23 16913.0 24 16913.0 25 16913.0 26 23717.0 27 30521.0 28 30521.0 29 30521.0 30 38671.0 31 46821.0 32 46821.0 33 46821.0 34 53468.0 35 60115.0 36 60115.0 37 60115.0 38 64856.0 39 69597.0 40 69597.0 41 69597.0 42 72093.5 43 74590.0 44 74590.0 45 74590.0 46 75658.5 47 76727.0 48 76727.0 49 76727.0 50 72335.5 51 67944.0 52 67944.0 53 67944.0 54 59058.5 55 50173.0 56 50173.0 57 50173.0 58 42138.5 59 34104.0 60 34104.0 61 34104.0 62 27237.5 63 20371.0 64 20371.0 65 20371.0 66 15709.0 67 11047.0 68 11047.0 69 11047.0 70 7994.5 71 4942.0 72 4942.0 73 4942.0 74 3494.0 75 2046.0 76 2046.0 77 2046.0 78 1397.5 79 749.0 80 749.0 81 749.0 82 491.5 83 234.0 84 234.0 85 234.0 86 152.5 87 71.0 88 71.0 89 71.0 90 43.0 91 15.0 92 15.0 93 15.0 94 9.5 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21542824105938385 2 0.12646945461553286 3 0.12750185832668007 4 0.07192412520992209 5 0.023057016215620955 6 0.022540814360047352 7 0.2658439556204058 8 0.2654998210500234 9 0.01875533408584093 10 0.035617928034578646 11 0.015313988382016905 12 0.01755086308950252 13 0.015141921096825701 14 0.017378795804311318 15 0.0147977865264433 16 0.01875533408584093 17 0.018411199515458523 18 0.014625719241252099 19 0.023745285356385763 20 0.016690526663546514 21 0.015486055667208107 22 0.017206728519120116 23 0.018239132230267324 24 0.015141921096825701 25 0.015486055667208107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 581168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.90215404194002 #Duplication Level Percentage of deduplicated Percentage of total 1 94.11184988551076 82.72634325808103 2 4.469453288376295 7.85749142876217 3 0.7108819351528659 1.8746416010831892 4 0.2566612331901729 0.9024430102590748 5 0.12667143945193593 0.5567346191709177 6 0.06848267861891542 0.36118649790987456 7 0.050653992166809075 0.31168165156002525 8 0.033938092134332985 0.23865851221453535 9 0.024763931921727544 0.19591226626210295 >10 0.1242954506490366 1.9369187691902146 >50 0.011958214313884587 0.7292578079595318 >100 0.008821577983006753 1.3757952628185381 >500 0.0015682805302774854 0.9329353147288166 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 985 0.16948627591333315 No Hit GTCCTACAGTGGACATTTCTAAATT 757 0.13025493488973927 No Hit GTACATGGGAAGCAGTGGTATCAAC 697 0.11993089777826721 No Hit CTGTAGGACGTGGAATATGGCAAGA 680 0.11700575393001679 No Hit GTCCTAAAGTGTGTATTTCTCATTT 622 0.10702585138892713 No Hit CCCATGTACTCTGCGTTGATACCAC 593 0.1020359001183823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAACAC 105 1.2951205E-9 13.617895 3 GGGTAGG 50 0.0014490418 13.358247 1 CCTATAC 50 0.0014594796 13.345537 3 TAGGACC 210 0.0 13.1491785 4 GTGTAGG 90 5.107304E-7 12.72214 1 TGTTACA 70 1.0535764E-4 12.257165 2 GTCCTAC 400 0.0 11.449926 1 TGAACTG 75 2.0356805E-4 11.420261 5 GGCGAGA 175 1.8189894E-12 11.378986 19 GTGTTAG 110 4.6751848E-7 11.276443 1 GTCTTAA 60 0.005680259 11.131873 1 AGGACCT 295 0.0 10.968613 5 CTATGAC 70 0.0014470894 10.894316 3 TCTACAC 105 3.3147626E-6 10.894316 3 CTACACT 105 3.3597298E-6 10.882078 4 TAACACT 70 0.0014607282 10.882077 4 GTCCTAA 195 1.8189894E-12 10.764889 1 CTTATAG 80 3.6459567E-4 10.724091 3 CTAAGAG 90 9.167188E-5 10.591696 3 TGGCGAG 310 0.0 10.400148 18 >>END_MODULE