FastQCFastQC Report
Thu 26 May 2016
SRR1926995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581168
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17780.3059356330699557No Hit
GGTATCAACGCAGAGTACTTTTTTT11270.19391983041048372No Hit
TATCAACGCAGAGTACTTTTTTTTT9740.16759353577622993No Hit
CTGTAGGACGTGGAATATGGCAAGA7970.13713762629738732No Hit
GTCCTACAGTGGACATTTCTAAATT7490.12887839660820966No Hit
GTACATGGGAAGCAGTGGTATCAAC7170.12337224348209123No Hit
CCCATGTACTCTGCGTTGATACCAC6540.11253200451504557No Hit
GTCCTAAAGTGTGTATTTCTCATTT6340.10909065881122154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT453.469479E-516.924496
GTCTTAT602.4879468E-514.2948471
GACGTGA1650.013.229237
AGTACTG751.4419278E-512.6944645
GGACGTG3500.012.5120336
TTAGGAC2600.012.4514133
AGGACGT3600.012.1655275
GTCCTAA1750.011.9804421
TAGGACG3700.011.836734
ATGGTAC657.8584417E-411.7189773
GTCCTAC3650.011.4880951
ATAGGAC752.026199E-411.4260033
CTAAGAC752.026199E-411.4260033
TAGGACC1501.6916601E-1011.4250174
TAGGACA1501.6916601E-1011.4250174
ATGGCAA3450.011.27666617
AGACTAT855.196601E-511.200036
CGTGAAA2300.011.1391459
CTCCTGG1201.2149576E-711.1182141
GGCGAGG1900.010.98714819
Produced by FastQC (version 0.11.5)