##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 581168 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.913233350769485 31.0 30.0 33.0 26.0 34.0 2 30.222575916086225 31.0 30.0 34.0 26.0 34.0 3 30.271239985684 31.0 30.0 34.0 26.0 34.0 4 33.85706026484596 37.0 35.0 37.0 28.0 37.0 5 33.85855552955428 37.0 35.0 37.0 30.0 37.0 6 33.7772193238444 37.0 35.0 37.0 29.0 37.0 7 33.784841904578364 37.0 35.0 37.0 29.0 37.0 8 33.80101106736778 37.0 35.0 37.0 29.0 37.0 9 35.34229861244941 39.0 35.0 39.0 30.0 39.0 10 35.2514384825042 39.0 35.0 39.0 30.0 39.0 11 35.25784282685901 39.0 35.0 39.0 30.0 39.0 12 35.171745519367896 39.0 35.0 39.0 29.0 39.0 13 35.171458167001624 39.0 35.0 39.0 29.0 39.0 14 36.285199804531565 40.0 36.0 41.0 29.0 41.0 15 36.249996558654296 40.0 36.0 41.0 29.0 41.0 16 36.234099262175484 40.0 36.0 41.0 29.0 41.0 17 36.21100611182997 40.0 36.0 41.0 29.0 41.0 18 36.193773573218074 40.0 36.0 41.0 29.0 41.0 19 36.26927841863282 40.0 37.0 41.0 29.0 41.0 20 36.22967197092751 40.0 37.0 41.0 29.0 41.0 21 36.18873716378052 40.0 37.0 41.0 29.0 41.0 22 36.09798027420643 40.0 37.0 41.0 29.0 41.0 23 36.02609056245354 40.0 37.0 41.0 29.0 41.0 24 35.94780855105581 40.0 36.0 41.0 28.0 41.0 25 35.84156732648735 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01980971718291258 1101 2 0.13831910908454503 1101 3 0.333243445733558 1101 4 0.22956943919597705 1101 5 0.18120189832719547 1101 6 0.16453607782452195 1101 7 0.22822303378275421 1101 8 -0.025519226749715074 1101 9 0.15230712805986002 1101 10 0.37922740231511654 1101 11 0.3040272829691304 1101 12 0.24412926542403568 1101 13 -0.0328537298374556 1101 14 0.16240421751572853 1101 15 0.26908643177237934 1101 16 0.18695592682656326 1101 17 0.07084767079206955 1101 18 0.12827916028435737 1101 19 0.16241275009633682 1101 20 0.2230574658875426 1101 21 0.278494585898585 1101 22 0.12549046012788523 1101 23 0.29460296946172093 1101 24 0.19198426154513726 1101 25 0.28434574101191146 1102 1 -0.3990625844014808 1102 2 -0.335634347659731 1102 3 -0.24985886259851142 1102 4 -0.4592202687709843 1102 5 -0.3858289160490074 1102 6 -0.4835021276081406 1102 7 -0.19937678209468146 1102 8 -0.22040014861793367 1102 9 -0.12147319070233209 1102 10 -0.24363648821564254 1102 11 -0.3236449788615303 1102 12 -0.14061236144530653 1102 13 -0.1467900297061604 1102 14 -0.18430589928702545 1102 15 -0.27045415103831516 1102 16 -0.008296378628593004 1102 17 -0.07242139400128877 1102 18 -0.19607863380174706 1102 19 -0.1671012604802513 1102 20 -0.36439029194944084 1102 21 -0.34561553195032246 1102 22 -0.4128143568741649 1102 23 -0.22622458824658054 1102 24 -0.39333065604432704 1102 25 -0.3258388000155108 1103 1 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13 0.09920012012116786 2211 14 0.11711951695695433 2211 15 0.19408655420316734 2211 16 0.33448625293321044 2211 17 0.29924108499501756 2211 18 0.39808846208605075 2211 19 0.21897822363414576 2211 20 0.2760675492873972 2211 21 0.17243768986191554 2211 22 0.2789280718459821 2211 23 0.13667092344733334 2211 24 0.29258441727709794 2211 25 0.2143875633697334 2212 1 -0.0401843100006829 2212 2 -0.22560993579898536 2212 3 -0.17788274579475427 2212 4 0.12300201192594784 2212 5 -0.23866402894041272 2212 6 -0.42796245325111926 2212 7 -0.17876006266123312 2212 8 -0.2777602468873326 2212 9 -0.48587373659029254 2212 10 -0.10259071328558633 2212 11 -0.19806563074552486 2212 12 -0.2215064024873783 2212 13 -0.2800457777365253 2212 14 -0.31806758642050426 2212 15 -0.42161272766297486 2212 16 -0.5973027883866422 2212 17 -0.42342352420824625 2212 18 -0.2641579430453618 2212 19 -0.36765528173926043 2212 20 -0.31385259709411173 2212 21 -0.4110670676489292 2212 22 -0.419291095038119 2212 23 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2214 9 -0.09322889197790829 2214 10 -0.37669195741562334 2214 11 -0.09908111469443526 2214 12 -0.16586956048809043 2214 13 -0.11230523511595436 2214 14 -0.1253158016610456 2214 15 -0.34881400557535613 2214 16 -0.2934686037828911 2214 17 -0.21506474225204641 2214 18 -0.1651376311291486 2214 19 -0.2740837716683231 2214 20 0.07810653297688219 2214 21 0.12536417408725953 2214 22 -0.10570701166954422 2214 23 0.03355186728672521 2214 24 -0.15988743021765828 2214 25 0.11879201181528742 2215 1 0.3622705853605339 2215 2 0.3379312966464987 2215 3 0.24884467590169024 2215 4 0.4369294132773405 2215 5 0.4397294199542685 2215 6 0.43426398049423653 2215 7 0.3508469572647499 2215 8 0.20075748371106528 2215 9 0.3500600402798213 2215 10 0.26314358260438553 2215 11 0.15645788050009202 2215 12 0.3181968473022607 2215 13 0.22526881309028823 2215 14 0.31702629381129555 2215 15 0.4575199983412048 2215 16 0.4180101770477904 2215 17 0.4202137280161651 2215 18 0.43407192724389887 2215 19 0.5562575574231872 2215 20 0.52291820157064 2215 21 0.3328584429398944 2215 22 0.2748200088244559 2215 23 0.3605347106041705 2215 24 0.43556262915457467 2215 25 0.2966413778802064 2216 1 -0.6541844139571751 2216 2 -0.5684135478144654 2216 3 -2.01792206024896 2216 4 -1.8438843022022162 2216 5 -0.9040257266443916 2216 6 -0.5933217883076125 2216 7 -0.6433853231431215 2216 8 -0.768668779352474 2216 9 -0.6688218008732036 2216 10 -0.7119104125678106 2216 11 -0.634184367957694 2216 12 -0.7078349427965023 2216 13 -0.6982615536063363 2216 14 -1.014798154826238 2216 15 -0.9009761831766809 2216 16 -1.001679356593911 2216 17 -0.9929916795003422 2216 18 -0.7696357412469155 2216 19 -1.0570805441112654 2216 20 -0.9565730439179987 2216 21 -1.002652734884208 2216 22 -0.7300560257889188 2216 23 -1.004843208546582 2216 24 -1.2049542187507924 2216 25 -1.3478327632554041 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13742.0 3 18.0 4 245.0 5 1468.0 6 2017.0 7 1511.0 8 1234.0 9 1027.0 10 877.0 11 810.0 12 736.0 13 714.0 14 719.0 15 743.0 16 714.0 17 843.0 18 882.0 19 1032.0 20 1320.0 21 1791.0 22 2275.0 23 2738.0 24 3198.0 25 3747.0 26 4551.0 27 5099.0 28 5841.0 29 6998.0 30 9205.0 31 11941.0 32 15224.0 33 20382.0 34 27569.0 35 38840.0 36 57234.0 37 99948.0 38 233935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.580145084075376 19.7892818321042 10.562437660532204 28.068135423288215 2 14.197054517268107 21.724743777452417 39.620015502540696 24.458186202738784 3 18.022898622055713 25.683422161722163 29.33832171996083 26.95535749626129 4 11.834099606310051 18.173230460039093 38.05629353302316 31.936376400627704 5 12.455561329666963 39.063527079626525 35.48451486566014 12.996396725046374 6 30.614179427898534 38.628158221433345 18.083682831948863 12.673979518719255 7 27.51120412504561 32.537226609022504 22.121865082370114 17.829704183561777 8 24.799906384926594 37.78916990619563 20.27850484337544 17.13241886550233 9 26.21191314493397 15.969203553224368 20.311612683400273 37.50727061844138 10 14.26668754527762 30.23710415960436 34.30929367773864 21.18691461737938 11 33.35925978824052 23.7540244798505 24.285402086620746 18.60131364528824 12 22.570487605950795 26.08067230361033 31.664274923171693 19.684565167267184 13 28.160575400922294 22.800089476219974 25.97150526533141 23.067829857526327 14 20.429411855800485 22.945728164193767 28.149051821593453 28.475808158412296 15 22.49248908206775 31.242149240992923 23.724692594389783 22.54066908254955 16 21.356450158647128 29.487029478770193 27.767549263202813 21.388971099379866 17 21.011136194697574 29.412493461443162 27.9432109131955 21.633159430663767 18 20.68114672718857 28.665426632964362 29.768896031605372 20.884530608241693 19 22.42264271715359 27.968208793685807 28.23198839576232 21.377160093398285 20 22.55873137140527 28.101238378905748 28.688164938235914 20.65186531145307 21 22.175874061492767 27.500176386874063 28.09524547029903 22.22870408133414 22 22.0881053327093 28.63199625581587 27.967988602263027 21.311909809211794 23 21.49898394093264 28.205489988247784 28.26536950652738 22.03015656429219 24 21.747928309886298 27.85201525204416 29.04530187484514 21.354754563224404 25 21.91891138482505 27.884062759187945 28.411816733259688 21.78520912272732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 258.0 1 258.0 2 254.0 3 250.0 4 250.0 5 250.0 6 430.0 7 610.0 8 610.0 9 610.0 10 990.0 11 1370.0 12 1370.0 13 1370.0 14 2169.5 15 2969.0 16 2969.0 17 2969.0 18 5298.0 19 7627.0 20 7627.0 21 7627.0 22 12003.0 23 16379.0 24 16379.0 25 16379.0 26 22841.0 27 29303.0 28 29303.0 29 29303.0 30 37652.5 31 46002.0 32 46002.0 33 46002.0 34 52727.5 35 59453.0 36 59453.0 37 59453.0 38 63958.5 39 68464.0 40 68464.0 41 68464.0 42 71463.0 43 74462.0 44 74462.0 45 74462.0 46 75819.5 47 77177.0 48 77177.0 49 77177.0 50 73403.5 51 69630.0 52 69630.0 53 69630.0 54 60303.0 55 50976.0 56 50976.0 57 50976.0 58 42984.0 59 34992.0 60 34992.0 61 34992.0 62 28185.0 63 21378.0 64 21378.0 65 21378.0 66 16374.5 67 11371.0 68 11371.0 69 11371.0 70 8276.5 71 5182.0 72 5182.0 73 5182.0 74 3682.5 75 2183.0 76 2183.0 77 2183.0 78 1473.5 79 764.0 80 764.0 81 764.0 82 511.5 83 259.0 84 259.0 85 259.0 86 174.5 87 90.0 88 90.0 89 90.0 90 52.5 91 15.0 92 15.0 93 15.0 94 9.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1164895520744432 2 0.10633758224816232 3 0.014625719241252099 4 0.0 5 0.0051620185557360344 6 0.3449949068083583 7 0.022712881645238554 8 0.011184373537428077 9 0.010668171681854473 10 0.005334085840927236 11 0.006366489552074444 12 3.441345703824023E-4 13 0.0013765382815296092 14 1.7206728519120114E-4 15 0.002408941992676816 16 6.882691407648046E-4 17 0.0 18 1.7206728519120114E-4 19 1.7206728519120114E-4 20 8.603364259560058E-4 21 0.010151969826280869 22 0.0 23 1.7206728519120114E-4 24 0.0 25 0.00447374941497123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 581168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.10400974485168 #Duplication Level Percentage of deduplicated Percentage of total 1 92.46850296510168 78.69440377433861 2 5.724468690911378 9.743504785108406 3 0.9360141567275255 2.3897567374637543 4 0.309184033940639 1.0525120414974676 5 0.16059247006928462 0.68335315688631 6 0.09685652017875951 0.4945726942287344 7 0.05665170521246247 0.337490109072471 8 0.04631069375674814 0.3152980586212109 9 0.02972543154842308 0.22767780745502278 >10 0.14640109354045414 2.2364125840893716 >50 0.012747329523774397 0.7373518216097744 >100 0.010521536990715424 1.6184806632319426 >500 0.0016186979985058072 0.9689531537172308 >1k 4.046744996264518E-4 0.5002326126796792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1778 0.3059356330699557 No Hit GGTATCAACGCAGAGTACTTTTTTT 1127 0.19391983041048372 No Hit TATCAACGCAGAGTACTTTTTTTTT 974 0.16759353577622993 No Hit CTGTAGGACGTGGAATATGGCAAGA 797 0.13713762629738732 No Hit GTCCTACAGTGGACATTTCTAAATT 749 0.12887839660820966 No Hit GTACATGGGAAGCAGTGGTATCAAC 717 0.12337224348209123 No Hit CCCATGTACTCTGCGTTGATACCAC 654 0.11253200451504557 No Hit GTCCTAAAGTGTGTATTTCTCATTT 634 0.10909065881122154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 45 3.469479E-5 16.92449 6 GTCTTAT 60 2.4879468E-5 14.294847 1 GACGTGA 165 0.0 13.22923 7 AGTACTG 75 1.4419278E-5 12.694464 5 GGACGTG 350 0.0 12.512033 6 TTAGGAC 260 0.0 12.451413 3 AGGACGT 360 0.0 12.165527 5 GTCCTAA 175 0.0 11.980442 1 TAGGACG 370 0.0 11.83673 4 ATGGTAC 65 7.8584417E-4 11.718977 3 GTCCTAC 365 0.0 11.488095 1 ATAGGAC 75 2.026199E-4 11.426003 3 CTAAGAC 75 2.026199E-4 11.426003 3 TAGGACC 150 1.6916601E-10 11.425017 4 TAGGACA 150 1.6916601E-10 11.425017 4 ATGGCAA 345 0.0 11.276666 17 AGACTAT 85 5.196601E-5 11.20003 6 CGTGAAA 230 0.0 11.139145 9 CTCCTGG 120 1.2149576E-7 11.118214 1 GGCGAGG 190 0.0 10.987148 19 >>END_MODULE