FastQCFastQC Report
Thu 26 May 2016
SRR1926994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597796
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9890.16544105346974555No Hit
GTATCAACGCAGAGTACTTTTTTTT9500.15891708877275862No Hit
GTCCTACAGTGTGCATTTCTCATTT8640.14453091021017203No Hit
CTGTAGGACGTGGAATATGGCAAGA7960.13315579227696406No Hit
GTCCTAAAGTGTGTATTTCTCATTT7650.12797007674858982No Hit
GTACATGGGAAGCAGTGGTATCAAC6360.10639080890470996No Hit
CTTTAGGACGTGAAATATGGCGAGG6130.10254334254494846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT350.00219371816.25709519
GTATAAC456.512744E-414.848731
CTCGAAC400.005335970314.22495718
GTCCTAA2750.013.5374121
TCCAACG500.00151764513.27662718
GTATTAG655.2022802E-513.2170011
CCAGTAC701.05262334E-412.2584743
CTTACTC701.05262334E-412.2584743
GGCGAGA3350.011.88951819
TAGGACC2900.011.8188644
TGTAGGA5950.011.6976282
GTAGGAC6000.011.6001473
CAGAACC752.0281968E-411.42494
TAGTCTG752.0408983E-411.4172285
CTGTAGG6050.011.3600671
GTATAGA1104.6507193E-711.28117751
TGGCGAG5150.011.23265418
ACAGGGC855.2647058E-511.1858488
AGAACAG2300.011.1690275
CTAATAC600.00571277411.1234293
Produced by FastQC (version 0.11.5)