##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597796 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.06441327810825 31.0 28.0 31.0 16.0 34.0 2 28.20053329229369 31.0 28.0 31.0 16.0 34.0 3 27.96871675287222 31.0 28.0 31.0 16.0 34.0 4 31.574958681556918 35.0 32.0 37.0 19.0 37.0 5 31.325990806228212 35.0 32.0 37.0 17.0 37.0 6 31.08878446828015 35.0 32.0 37.0 15.0 37.0 7 30.859699629974106 35.0 32.0 37.0 11.0 37.0 8 30.65748516216234 35.0 31.0 37.0 11.0 37.0 9 32.87589244491432 37.0 32.0 39.0 17.0 39.0 10 33.128291256549055 37.0 33.0 39.0 17.0 39.0 11 33.27164283467939 37.0 33.0 39.0 17.0 39.0 12 33.18553988317085 38.0 33.0 39.0 17.0 39.0 13 33.2667732805171 38.0 33.0 39.0 17.0 39.0 14 34.247101017738494 39.0 34.0 40.0 17.0 41.0 15 33.860786957423606 39.0 33.0 40.0 11.0 41.0 16 33.92289008290454 39.0 33.0 40.0 16.0 41.0 17 34.138436858058604 39.0 34.0 40.0 17.0 41.0 18 34.169069381528146 39.0 34.0 41.0 17.0 41.0 19 33.57363214206853 39.0 33.0 40.0 10.0 41.0 20 33.882148759777586 39.0 34.0 41.0 10.0 41.0 21 33.85761028845961 39.0 34.0 41.0 10.0 41.0 22 33.86047246886898 39.0 34.0 41.0 2.0 41.0 23 33.84063125213284 39.0 34.0 41.0 2.0 41.0 24 32.5488828965065 38.0 32.0 40.0 2.0 41.0 25 33.11097765793013 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.09107833947142652 1101 2 -0.08043869993301556 1101 3 -0.9242503117550918 1101 4 -0.33995705772023754 1101 5 -0.5352204532328706 1101 6 -2.0649257051105145 1101 7 -0.5049674621228597 1101 8 -0.026574664838374673 1101 9 -0.025398427250671318 1101 10 -8.79751339166248 1101 11 -3.9048890214659124 1101 12 -1.4766536749166868 1101 13 -0.591419699218342 1101 14 -0.3697441002739268 1101 15 -1.670299388610303 1101 16 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24 2.3219872997049578 2208 25 1.796561033128718 2209 1 0.562730532691873 2209 2 0.5112336686380878 2209 3 -0.42349480941731343 2209 4 0.5421845775859531 2209 5 0.8448542417152467 2209 6 1.1735992122118724 2209 7 -0.5405616005650984 2209 8 -1.4204764780439838 2209 9 -0.3109070371263698 2209 10 -0.9573947920502093 2209 11 0.10524611366922443 2209 12 0.5858391977027679 2209 13 0.5715264748037825 2209 14 0.3150403677383906 2209 15 0.8147473670060705 2209 16 0.9293784452735849 2209 17 0.6926366058446405 2209 18 0.9195362981499784 2209 19 -1.2715537567244297 2209 20 0.5325613520706014 2209 21 0.8995527641016423 2209 22 0.9309801609067989 2209 23 0.8768452080809013 2209 24 0.906961017791204 2209 25 1.1705285293741596 2210 1 0.5039512695768735 2210 2 0.5905184721923824 2210 3 0.826938961630443 2210 4 0.9971019639182224 2210 5 0.703241139200685 2210 6 0.7013276978251319 2210 7 1.2271934370941118 2210 8 1.2189874860976104 2210 9 0.5020390569625164 2210 10 0.9522691011106659 2210 11 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23 -1.1923426211509565 2211 24 0.935913765648742 2211 25 0.6866920118282351 2212 1 -1.2300725025188157 2212 2 -0.7810770301772827 2212 3 -3.360180996790181 2212 4 -2.557816933973154 2212 5 -1.1240184375016824 2212 6 -0.42961652182282606 2212 7 -0.08814980003907635 2212 8 -0.1965044462062302 2212 9 -0.2911768720209267 2212 10 -0.319579470023335 2212 11 -0.5003492462761869 2212 12 -0.5543805829915982 2212 13 -0.6882792250696781 2212 14 -0.7217121122982348 2212 15 -1.790610462222297 2212 16 -0.980448416427393 2212 17 -0.8518701724182733 2212 18 -0.9943588169228619 2212 19 -0.09870373361916762 2212 20 -1.3563649659009727 2212 21 -1.3584369054715353 2212 22 -0.7572510558119134 2212 23 -0.6294795871028427 2212 24 0.6419253234025462 2212 25 -0.09770735158407717 2213 1 -0.41293067961645136 2213 2 -0.4710396667534944 2213 3 0.3267110154257473 2213 4 -6.302932228012484 2213 5 -1.3072760454104113 2213 6 -3.367166970766416 2213 7 -1.128127207233824 2213 8 -3.0847761590154796 2213 9 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20 -1.085191103522476 2214 21 -0.4821770218342323 2214 22 -0.1875908257131229 2214 23 -0.5887807855427241 2214 24 -2.1105246095773786 2214 25 -1.0619407273738872 2215 1 0.21567573070212376 2215 2 -0.2791988417846092 2215 3 0.04640100556620297 2215 4 0.40560112471667153 2215 5 0.6289723180672588 2215 6 0.7713292874566058 2215 7 0.2238645010530398 2215 8 -0.6425029688879498 2215 9 -0.5479321438698719 2215 10 -0.8328509160656097 2215 11 -0.6763973588291208 2215 12 -1.0175300383576626 2215 13 -0.7710782344931815 2215 14 -0.733482839012666 2215 15 -0.937607700898738 2215 16 -0.47698506144978836 2215 17 -1.0399342139622547 2215 18 -1.7452261818225097 2215 19 -0.40898025260148785 2215 20 -4.073762728760865 2215 21 -2.3977750831607736 2215 22 -1.7378108902545932 2215 23 -1.526095795685528 2215 24 -1.3414283925819994 2215 25 -1.0969992155487063 2216 1 -0.2877625366759169 2216 2 -0.9312521825102849 2216 3 -0.015365750815380608 2216 4 -0.11094683001073946 2216 5 -0.05947142116229642 2216 6 -1.4104706517220578 2216 7 -9.440698588670845 2216 8 -11.273515023511305 2216 9 -5.996701546186465 2216 10 -3.3390054560473637 2216 11 -2.1292318606630722 2216 12 -1.728270796721116 2216 13 -1.4125343883090977 2216 14 -1.6538782604486713 2216 15 -2.657844035234035 2216 16 -1.7804680138912232 2216 17 -2.118758872669744 2216 18 -1.6863836077146104 2216 19 -0.9953196563455222 2216 20 -2.1048943573038628 2216 21 -4.76838389646203 2216 22 -3.346325256709349 2216 23 -2.9538208087703914 2216 24 -3.221863782324707 2216 25 -2.6007177989494465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35807.0 3 2.0 4 249.0 5 1681.0 6 2214.0 7 1767.0 8 1375.0 9 1167.0 10 1101.0 11 1123.0 12 1064.0 13 1072.0 14 1144.0 15 1103.0 16 1263.0 17 1319.0 18 1596.0 19 1936.0 20 2454.0 21 3247.0 22 4141.0 23 4909.0 24 5566.0 25 6557.0 26 7387.0 27 8001.0 28 9169.0 29 10493.0 30 12761.0 31 15460.0 32 20094.0 33 26119.0 34 35315.0 35 48675.0 36 70469.0 37 109501.0 38 140495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13547011126536 19.443438428468912 13.272536434545248 25.148555025720476 2 13.461515913388823 20.35031062975178 43.3593573339274 22.828816122931997 3 18.489496526759947 25.088233865388705 29.96283038494589 26.459439222905463 4 11.12801764975678 17.689093776050296 40.01911610607263 31.163772468120293 5 12.098239532058853 39.27824495300176 36.225283679280956 12.398231835658427 6 30.609434735688534 38.30127910186804 19.098187172172633 11.991098990270794 7 26.855286091246576 32.52896643466679 22.901558576195104 17.714188897891535 8 23.54988652051605 38.3020516889404 21.28667115104933 16.861390639494214 9 25.592982420917554 16.294823983309325 21.176880001472313 36.93531359430081 10 13.603923648819375 30.513029465852977 35.660894199699804 20.222152685627844 11 33.188781869498804 23.71095730210754 24.79124526736165 18.309015561032005 12 22.187459741980604 26.17285726953173 32.63589016022901 19.00379282825866 13 28.164232941617367 23.145762507696013 26.091997483737988 22.59800706694863 14 19.61643157220225 23.584121210701635 28.76445324851975 28.034993968576366 15 22.308755328617604 32.19405202470739 23.767140247207706 21.730052399467308 16 20.607402127848243 29.79874283285901 29.156272534562056 20.43758250473069 17 20.592442633784792 29.970971816729268 28.673152694936384 20.76343285454956 18 20.212609332860424 29.096259762161292 30.833037763754024 19.858093141224263 19 22.64550919643888 28.170567045410817 28.374356048501113 20.80956770964919 20 22.178276130914735 28.64219962456207 29.407798535055928 19.77172570946726 21 21.801454534503787 28.01978193693671 28.169183744146476 22.009579784413024 22 21.444764372941076 29.544606602275714 28.53641356995029 20.47421545483292 23 21.028609670403213 29.11109252133177 28.651664714739837 21.20863309352518 24 21.907650712972366 28.3994385227594 29.318280300279902 20.37463046398833 25 21.466250637845796 28.4869879457601 29.052140234058037 20.99462118233606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 197.0 2 257.5 3 318.0 4 318.0 5 318.0 6 490.5 7 663.0 8 663.0 9 663.0 10 1096.0 11 1529.0 12 1529.0 13 1529.0 14 2617.5 15 3706.0 16 3706.0 17 3706.0 18 6370.5 19 9035.0 20 9035.0 21 9035.0 22 13890.5 23 18746.0 24 18746.0 25 18746.0 26 25923.0 27 33100.0 28 33100.0 29 33100.0 30 42578.0 31 52056.0 32 52056.0 33 52056.0 34 58737.5 35 65419.0 36 65419.0 37 65419.0 38 70246.5 39 75074.0 40 75074.0 41 75074.0 42 77374.0 43 79674.0 44 79674.0 45 79674.0 46 80040.5 47 80407.0 48 80407.0 49 80407.0 50 74743.5 51 69080.0 52 69080.0 53 69080.0 54 58515.5 55 47951.0 56 47951.0 57 47951.0 58 39083.0 59 30215.0 60 30215.0 61 30215.0 62 23637.5 63 17060.0 64 17060.0 65 17060.0 66 12665.0 67 8270.0 68 8270.0 69 8270.0 70 5876.0 71 3482.0 72 3482.0 73 3482.0 74 2355.5 75 1229.0 76 1229.0 77 1229.0 78 819.0 79 409.0 80 409.0 81 409.0 82 269.5 83 130.0 84 130.0 85 130.0 86 79.5 87 29.0 88 29.0 89 29.0 90 19.5 91 10.0 92 10.0 93 10.0 94 6.5 95 3.0 96 3.0 97 3.0 98 3.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2315171061699978 2 0.1309811373779684 3 0.13432676029950016 4 0.06590877155417567 5 0.019070050652731034 6 0.018568207214501267 7 0.2801959196782849 8 0.2765157344645999 9 0.016226271169429037 10 0.03161613660847513 11 0.01421889741650998 12 0.014888022000816332 13 0.014051616270433392 14 0.015557146585122684 15 0.013382491686127039 16 0.017229958045888564 17 0.016895395753735387 18 0.013382491686127039 19 0.019906456383113974 20 0.01572442773119927 21 0.013215210540050454 22 0.015557146585122684 23 0.016058990023352447 24 0.014553459708663155 25 0.013549772832203627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 597796.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.00027447302017 #Duplication Level Percentage of deduplicated Percentage of total 1 95.19423699976441 84.72313221228765 2 3.768519337192814 6.707985107340892 3 0.4976177048037062 1.3286433693049255 4 0.17212890941870512 0.6127808073202554 5 0.08420360184160458 0.3747071837759857 6 0.05896272778802864 0.31486193740875135 7 0.0352468894537919 0.21958879849953758 8 0.027542706122503455 0.19610467237060328 9 0.022229525805851046 0.17805905083132453 >10 0.11617171295303869 1.9658026550106111 >50 0.01185219153190596 0.7002172352154907 >100 0.009406411103228481 1.460364877680561 >500 0.001881282220461976 1.2177520929534769 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 989 0.16544105346974555 No Hit GTATCAACGCAGAGTACTTTTTTTT 950 0.15891708877275862 No Hit GTCCTACAGTGTGCATTTCTCATTT 864 0.14453091021017203 No Hit CTGTAGGACGTGGAATATGGCAAGA 796 0.13315579227696406 No Hit GTCCTAAAGTGTGTATTTCTCATTT 765 0.12797007674858982 No Hit GTACATGGGAAGCAGTGGTATCAAC 636 0.10639080890470996 No Hit CTTTAGGACGTGAAATATGGCGAGG 613 0.10254334254494846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.67281146076588E-4 0.0 13 0.0 0.0 0.0 1.67281146076588E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGAACT 35 0.002193718 16.257095 19 GTATAAC 45 6.512744E-4 14.84873 1 CTCGAAC 40 0.0053359703 14.224957 18 GTCCTAA 275 0.0 13.537412 1 TCCAACG 50 0.001517645 13.276627 18 GTATTAG 65 5.2022802E-5 13.217001 1 CCAGTAC 70 1.05262334E-4 12.258474 3 CTTACTC 70 1.05262334E-4 12.258474 3 GGCGAGA 335 0.0 11.889518 19 TAGGACC 290 0.0 11.818864 4 TGTAGGA 595 0.0 11.697628 2 GTAGGAC 600 0.0 11.600147 3 CAGAACC 75 2.0281968E-4 11.4249 4 TAGTCTG 75 2.0408983E-4 11.417228 5 CTGTAGG 605 0.0 11.360067 1 GTATAGA 110 4.6507193E-7 11.2811775 1 TGGCGAG 515 0.0 11.232654 18 ACAGGGC 85 5.2647058E-5 11.185848 8 AGAACAG 230 0.0 11.169027 5 CTAATAC 60 0.005712774 11.123429 3 >>END_MODULE