FastQCFastQC Report
Thu 26 May 2016
SRR1926994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597796
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16640.2783558270714424No Hit
CTGTAGGACGTGGAATATGGCAAGA10210.17079405014419635No Hit
GTCCTACAGTGGACATTTCTAAATT10100.16895395753735387No Hit
GGTATCAACGCAGAGTACTTTTTTT10100.16895395753735387No Hit
TATCAACGCAGAGTACTTTTTTTTT8540.14285809874940616No Hit
GTCCTACAGTGTGCATTTCTCATTT8030.13432676029950016No Hit
GTCCTAAAGTGTGTATTTCTCATTT7190.12027514402906678No Hit
CTTTAGGACGTGAAATATGGCGAGG7090.1186023325683009No Hit
GTACATGGGAAGCAGTGGTATCAAC6910.11559127193892231No Hit
CTGTAGGACCTGGAATATGGCGAGA6530.10923458838801196No Hit
CACACACACACACACACACACACAC6110.10220878025279526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT952.8376235E-1015.0638461
TTAAGAC653.2610533E-614.6590893
GGGTAAT400.005127476514.3106551
TGTAGGA6200.013.693992
GTAGGAC5850.013.6818173
GTCCTAT1051.2787496E-913.6291941
CTGTAGG6450.013.6080641
TAGGACC2400.013.4985784
GTACTAG655.2288433E-513.2098341
AGGACGT4850.012.573575
GACGTGG2800.012.5394697
GGACGTG4750.012.4370796
TAGGACT1001.3817953E-712.3869314
GTGCTAA550.00296864912.1423731
TAGGACG5000.012.0057954
TTACACT802.7733427E-511.910514
AGGACCT3700.011.8461285
TAGGACA2350.011.7584614
AAGACAG1302.4792826E-911.7272715
TAGCCTC752.0131023E-411.434095