FastQCFastQC Report
Thu 26 May 2016
SRR1926993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564388
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC9740.17257631274938517No Hit
GTCCTACAGTGGACATTTCTAAATT9610.17027293280509154No Hit
GTATCAACGCAGAGTACTTTTTTTT9500.16832391900607385No Hit
GTCCTAAAGTGTGTATTTCTCATTT8030.14227800732829188No Hit
CTGTAGGACGTGGAATATGGCAAGA7820.13855716280289446No Hit
CCCATGTACTCTGCGTTGATACCAC7400.13111547375209961No Hit
GTCCTACAGTGTGCATTTCTCATTT6330.11215688497983657No Hit
CTTTAGGACGTGAAATATGGCGAGG6200.10985350503554293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGAC250.005978287719.0269327
TAGGACC1400.015.6306554
TAAGGGT551.1098098E-515.5688744
TGTAGGA5000.013.7189042
CTGTAGG5250.013.4381261
GGCGAGG1800.013.17567219
GTGTAGG801.9149957E-613.1089831
GGACGTG4600.012.8224986
GTAGGAC5350.012.8214043
GTTAAGA603.964199E-412.71174051
AGGACGT4850.012.3576985
TAGCACT701.07332526E-412.2326874
TGGCGAG4450.012.15122518
ATCCTAC550.002983797312.1339341
CCAATAC550.00299976712.1252943
TAGGACG4900.012.0385184
AGGACCT3300.011.8197615
GACGTGG2550.011.565397
TGGAATA6400.011.56165211
GGACCTG3050.011.5409276
Produced by FastQC (version 0.11.5)