FastQCFastQC Report
Thu 26 May 2016
SRR1926993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564388
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15930.2822526347122901No Hit
GTACATGGGAAGCAGTGGTATCAAC10730.19011743694054445No Hit
GGTATCAACGCAGAGTACTTTTTTT10300.18249856481711163No Hit
CTGTAGGACGTGGAATATGGCAAGA9810.17381659425785098No Hit
GTCCTACAGTGGACATTTCTAAATT9480.1679695528607979No Hit
CCCATGTACTCTGCGTTGATACCAC8930.15822448386570942No Hit
TATCAACGCAGAGTACTTTTTTTTT8820.1562754700666917No Hit
GTCCTAAAGTGTGTATTTCTCATTT8010.14192364118301593No Hit
CTTTAGGACGTGAAATATGGCGAGG7620.135013501350135No Hit
GTCCTACAGTGTGCATTTCTCATTT6450.11428308185149223No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACC400.005214890514.2740543
TCCTAGA1204.9112714E-1113.4906392
TAGGACC2650.013.2865414
GACGTGG2500.012.9096957
AGGACCC604.0271165E-412.6880485
GTATAGG853.8115359E-612.3280121
CGTGGAA2700.012.3028139
GTTCAAG701.0608928E-412.2479611
TAAGCCT550.003025802612.1113194
GCGCCAC550.003088792312.07805413
ACGTGGA2700.011.95130358
GTAGGAC5750.011.9157323
TGTAGGG657.8499713E-411.7203742
TAACACC657.8969524E-411.7120444
CGCCACC658.0813287E-411.67987714
GTATTAG752.0170811E-411.4314311
CTGTAGG6200.011.3699711
TGTAGGA6400.011.308332
GTCCTAC5150.011.283451
GGCGAGA3450.011.2808519
Produced by FastQC (version 0.11.5)