##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564388 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.875739739328264 31.0 30.0 33.0 26.0 34.0 2 30.17975931451413 31.0 30.0 34.0 26.0 34.0 3 30.24043211407755 31.0 30.0 34.0 26.0 34.0 4 33.867068399753364 35.0 35.0 37.0 29.0 37.0 5 33.875504085841655 37.0 35.0 37.0 30.0 37.0 6 33.840471448719676 37.0 35.0 37.0 30.0 37.0 7 33.82845134907191 37.0 35.0 37.0 29.0 37.0 8 33.8618592174178 37.0 35.0 37.0 30.0 37.0 9 35.39021205270134 39.0 35.0 39.0 30.0 39.0 10 35.27501824985648 39.0 35.0 39.0 30.0 39.0 11 35.32701616618355 39.0 35.0 39.0 30.0 39.0 12 35.222187573088014 39.0 35.0 39.0 29.0 39.0 13 35.23429803610282 39.0 35.0 39.0 30.0 39.0 14 36.33597631416685 40.0 36.0 41.0 29.0 41.0 15 36.32123291069264 40.0 36.0 41.0 29.0 41.0 16 36.29379965555611 40.0 36.0 41.0 29.0 41.0 17 36.28388271898056 40.0 36.0 41.0 29.0 41.0 18 36.25340191499465 40.0 36.0 41.0 29.0 41.0 19 36.322501541492734 40.0 37.0 41.0 29.0 41.0 20 36.27962146608362 40.0 37.0 41.0 29.0 41.0 21 36.2352973486325 40.0 37.0 41.0 29.0 41.0 22 36.14837133319631 40.0 37.0 41.0 29.0 41.0 23 36.08060057974301 40.0 37.0 41.0 29.0 41.0 24 36.01499677526808 40.0 36.0 41.0 29.0 41.0 25 35.90209394955244 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.017980503348784538 1101 2 0.2856313052150625 1101 3 0.4063311354746979 1101 4 0.22665770087279924 1101 5 0.07204231445964382 1101 6 0.22901910211836451 1101 7 0.22867485921484842 1101 8 0.33524058278086244 1101 9 0.2918993328603108 1101 10 0.3936861938976861 1101 11 0.492859171891709 1101 12 0.5541530009761146 1101 13 0.3615121543608666 1101 14 0.43985712496135676 1101 15 0.17832168233926637 1101 16 0.27544149287552244 1101 17 0.37143030890776174 1101 18 0.39565794071261706 1101 19 0.23568461712932276 1101 20 0.22702075611521622 1101 21 0.19013902418117823 1101 22 0.2638436898094767 1101 23 0.11902832353910497 1101 24 0.2716522939892414 1101 25 0.22142688605834593 1102 1 -0.7555040277248608 1102 2 -0.7572414154571661 1102 3 -0.5527627698952422 1102 4 -0.48177258762482467 1102 5 -0.6971705028652266 1102 6 -0.8158570932449862 1102 7 -0.5157374526011509 1102 8 -0.47536282066953817 1102 9 -0.524512509476601 1102 10 -0.4982148824922916 1102 11 -0.347752679452654 1102 12 -0.4699842288516365 1102 13 -0.6403766800890764 1102 14 -0.6273370687833761 1102 15 -0.550477720439666 1102 16 -0.45513619840649966 1102 17 -0.5333418640843561 1102 18 -0.5531224372453352 1102 19 -0.4656830136526722 1102 20 -0.5090091704467881 1102 21 -0.5965809956897985 1102 22 -0.6553431287464093 1102 23 -0.6184632660979688 1102 24 -0.4860637448628822 1102 25 -0.4223517654721647 1103 1 -0.3209229981458712 1103 2 -0.4443472337480081 1103 3 -0.31848614650809637 1103 4 -0.2893659960288133 1103 5 -0.34864296990589594 1103 6 -0.31601915510399436 1103 7 -0.2535470245147522 1103 8 -0.2644838781771881 1103 9 -0.32926848483239013 1103 10 -0.25041419697062395 1103 11 0.050134844215129704 1103 12 -0.19538391391142085 1103 13 -0.07518594678617063 1103 14 0.04533308941600467 1103 15 0.030342528649413225 1103 16 0.05113829656823299 1103 17 -0.015227399793339202 1103 18 -0.1310282739031905 1103 19 0.07650573979451991 1103 20 -0.022253675874509327 1103 21 -0.03861822129881176 1103 22 0.11317986648086276 1103 23 0.023601510027077666 1103 24 0.07464867906933392 1103 25 -0.07614711915866934 1104 1 0.00468223537176371 1104 2 -0.09949985550373341 1104 3 0.14106726502908984 1104 4 -0.07432206375986539 1104 5 0.06883648200089709 1104 6 0.10265280198202476 1104 7 -0.018647813485898723 1104 8 -0.07606130728187566 1104 9 -0.37666617653131595 1104 10 -0.1361659884294255 1104 11 -0.11026723125707605 1104 12 -0.07301100417177508 1104 13 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11 0.3220301311916245 1107 12 0.5395837050469154 1107 13 0.16180109298508683 1107 14 0.2794843467691166 1107 15 0.45077396338865583 1107 16 0.5609789042433349 1107 17 0.3797405837283989 1107 18 0.17304494084287825 1107 19 -0.04153988791855312 1107 20 0.1489270668452889 1107 21 0.09060387853426732 1107 22 0.010926343887049939 1107 23 -0.017372811689696732 1107 24 0.2549356946231711 1107 25 0.3635475850371961 1108 1 0.4908399152374301 1108 2 0.31204931484223764 1108 3 0.37101528088720315 1108 4 0.36449866337435566 1108 5 0.5727513868039296 1108 6 0.6364250588663012 1108 7 0.48659485964704174 1108 8 0.48354610247010754 1108 9 0.4730693427468111 1108 10 0.32530190640058976 1108 11 0.5001011641013662 1108 12 0.4921314559817631 1108 13 0.47435055069583854 1108 14 0.37096365599036574 1108 15 0.38500749170820825 1108 16 0.4748474928431037 1108 17 0.5667088325246041 1108 18 0.3703055655228624 1108 19 0.3581650467326227 1108 20 0.2810250564701562 1108 21 0.48624358331375817 1108 22 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0.4782297255277186 2214 10 0.390271806268494 2214 11 0.2853062181000965 2214 12 0.29471341203657886 2214 13 0.19758563424009168 2214 14 0.2659131865896427 2214 15 0.10075787741341458 2214 16 0.14168138246586892 2214 17 0.1627948069722578 2214 18 0.2482941506653944 2214 19 0.3381713995243203 2214 20 0.3341994355604001 2214 21 0.41837711294499513 2214 22 0.3500088784473405 2214 23 0.42175957711446443 2214 24 0.16870504384109353 2214 25 0.2769780236317487 2215 1 0.5566467133386723 2215 2 0.43948772653968504 2215 3 0.3900028419557735 2215 4 0.5219949386246938 2215 5 0.5448677375887243 2215 6 0.3889197749191027 2215 7 0.3147626293015975 2215 8 0.3674551839295219 2215 9 0.25122152042602863 2215 10 0.5259533044810496 2215 11 0.2467662138224469 2215 12 0.4901997834825451 2215 13 0.42048893813910126 2215 14 0.31057940042056487 2215 15 0.6052787920789484 2215 16 0.534989206574437 2215 17 0.5791737505801109 2215 18 0.4149592504263637 2215 19 0.36546728851218546 2215 20 0.33094536415180187 2215 21 0.39282166446133004 2215 22 0.4211377791611426 2215 23 0.5276549814583973 2215 24 0.41191544558787996 2215 25 0.26538370491842755 2216 1 -0.6076669866957403 2216 2 -0.4593067429374784 2216 3 -2.422000288528853 2216 4 -1.7951968843926949 2216 5 -0.9222738090617426 2216 6 -0.39834076933940565 2216 7 -0.662774061423427 2216 8 -0.562402854226633 2216 9 -0.7242820699556916 2216 10 -0.5077643174774167 2216 11 -0.5998914794415029 2216 12 -0.4913835117811942 2216 13 -0.7621937010211042 2216 14 -1.0423313011582707 2216 15 -0.768262978947412 2216 16 -0.795556166468856 2216 17 -1.055932503408016 2216 18 -0.9749342812526507 2216 19 -1.0971536717725456 2216 20 -0.888143243505148 2216 21 -1.0540684881069282 2216 22 -1.0626396722863376 2216 23 -0.985898816703731 2216 24 -0.9318965195750621 2216 25 -0.8116671238149138 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13144.0 3 12.0 4 191.0 5 1313.0 6 1923.0 7 1438.0 8 1099.0 9 968.0 10 844.0 11 767.0 12 758.0 13 669.0 14 691.0 15 693.0 16 695.0 17 696.0 18 780.0 19 1038.0 20 1272.0 21 1697.0 22 2191.0 23 2568.0 24 3119.0 25 3681.0 26 4309.0 27 4886.0 28 5672.0 29 6917.0 30 8902.0 31 11535.0 32 14653.0 33 19867.0 34 26812.0 35 38060.0 36 56097.0 37 97701.0 38 226730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.683991683991685 19.7540675356375 10.71235977379322 27.849581006577594 2 14.082608510577918 21.057933794003716 40.42542247825532 24.434035217163046 3 18.34312396445248 25.33584965843545 29.320503619432408 27.00052275767966 4 11.812441086628347 17.75374387832484 38.14468060979326 32.28913442525355 5 12.397205295267325 39.092730968028874 35.66119077952983 12.848872957173969 6 31.066471578595547 38.50156716404643 18.000430236754664 12.431531020603364 7 27.78677659001333 32.114476706269315 22.00126889159838 18.097477812118978 8 24.76950583965793 38.41805606993059 19.90214962495415 16.91028846545733 9 26.155086562826714 15.782786667375474 19.987773111477328 38.07435365832049 10 14.204914939196719 30.423434645554888 34.40847259112518 20.96317782412321 11 33.82763869225549 23.344809439348218 24.261598515843584 18.56595335255271 12 22.714104733470947 25.687252496079804 32.03292078988634 19.565721980562913 13 28.682902628524854 22.552512912285053 25.84104822062954 22.92353623856056 14 20.31832767232413 23.03082813743052 27.95953840184129 28.691305788404055 15 22.65904761904762 31.521949058693245 23.51734219269103 22.301661129568107 16 21.3683921436608 29.193015406150057 28.27024105885167 21.168351391337474 17 21.02674410775604 29.493112869560907 28.047293873342007 21.432849149341056 18 20.855621605818722 28.48675992451961 30.007353136600017 20.65026533306165 19 22.655456824685277 27.78263065106266 28.049469776836734 21.512442747415328 20 22.821518682315585 28.072021885808248 28.65123745534955 20.45522197652662 21 22.307241117169543 27.267378911573065 27.785702767406377 22.639677203851015 22 22.20852004032694 28.82135839415153 27.69376332197588 21.27635824354565 23 21.429621872934707 28.42694817563126 28.25773804145028 21.885691909983752 24 22.188891999447186 27.741829173650657 28.897421268422676 21.17185755847948 25 22.024378013363787 27.996264802139052 28.36482322485076 21.614533959646394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 216.0 1 216.0 2 224.5 3 233.0 4 233.0 5 233.0 6 410.0 7 587.0 8 587.0 9 587.0 10 979.5 11 1372.0 12 1372.0 13 1372.0 14 2151.0 15 2930.0 16 2930.0 17 2930.0 18 5016.5 19 7103.0 20 7103.0 21 7103.0 22 11188.5 23 15274.0 24 15274.0 25 15274.0 26 21703.5 27 28133.0 28 28133.0 29 28133.0 30 36387.5 31 44642.0 32 44642.0 33 44642.0 34 50830.5 35 57019.0 36 57019.0 37 57019.0 38 61376.5 39 65734.0 40 65734.0 41 65734.0 42 68728.0 43 71722.0 44 71722.0 45 71722.0 46 74033.5 47 76345.0 48 76345.0 49 76345.0 50 72528.5 51 68712.0 52 68712.0 53 68712.0 54 59426.0 55 50140.0 56 50140.0 57 50140.0 58 42281.5 59 34423.0 60 34423.0 61 34423.0 62 27587.5 63 20752.0 64 20752.0 65 20752.0 66 15888.5 67 11025.0 68 11025.0 69 11025.0 70 8009.5 71 4994.0 72 4994.0 73 4994.0 74 3483.0 75 1972.0 76 1972.0 77 1972.0 78 1351.5 79 731.0 80 731.0 81 731.0 82 484.5 83 238.0 84 238.0 85 238.0 86 155.5 87 73.0 88 73.0 89 73.0 90 44.0 91 15.0 92 15.0 93 15.0 94 9.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11623209565050995 2 0.10134871754892023 3 0.012934364302571989 4 0.0 5 0.006201407542329036 6 0.3378881195206135 7 0.020553236426004807 8 0.01009943514036443 9 0.010276618213002403 10 0.004783942961225257 11 0.004783942961225257 12 5.315492179139175E-4 13 5.315492179139175E-4 14 1.7718307263797246E-4 15 0.002303379944293642 16 5.315492179139175E-4 17 3.543661452759449E-4 18 5.315492179139175E-4 19 5.315492179139175E-4 20 7.087322905518898E-4 21 0.012225632012020099 22 1.7718307263797246E-4 23 1.7718307263797246E-4 24 3.543661452759449E-4 25 0.004783942961225257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 564388.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.01978066617683 #Duplication Level Percentage of deduplicated Percentage of total 1 92.42582985039519 77.65597951919582 2 5.791641436556659 9.732248863932636 3 0.8987709514906227 2.265436146401195 4 0.3141143651379382 1.0556728025193978 5 0.14402116480857477 0.6050313339251877 6 0.10194041964844366 0.5139007019928155 7 0.05793059526748397 0.3407121135764545 8 0.042607656173817565 0.28639087411392217 9 0.028335232109346117 0.21426479882572244 >10 0.16478125398473054 2.5394065985556717 >50 0.013956274007607426 0.7804911551895427 >100 0.01374476351684246 2.213013475543389 >500 0.0016916632020260447 1.140796365306041 >1k 6.343737007597668E-4 0.6566552509221504 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1593 0.2822526347122901 No Hit GTACATGGGAAGCAGTGGTATCAAC 1073 0.19011743694054445 No Hit GGTATCAACGCAGAGTACTTTTTTT 1030 0.18249856481711163 No Hit CTGTAGGACGTGGAATATGGCAAGA 981 0.17381659425785098 No Hit GTCCTACAGTGGACATTTCTAAATT 948 0.1679695528607979 No Hit CCCATGTACTCTGCGTTGATACCAC 893 0.15822448386570942 No Hit TATCAACGCAGAGTACTTTTTTTTT 882 0.1562754700666917 No Hit GTCCTAAAGTGTGTATTTCTCATTT 801 0.14192364118301593 No Hit CTTTAGGACGTGAAATATGGCGAGG 762 0.135013501350135 No Hit GTCCTACAGTGTGCATTTCTCATTT 645 0.11428308185149223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.7718307263797246E-4 0.0 0.0 0.0 6 0.0 1.7718307263797246E-4 0.0 0.0 0.0 7 0.0 1.7718307263797246E-4 0.0 0.0 0.0 8 0.0 1.7718307263797246E-4 0.0 0.0 0.0 9 0.0 1.7718307263797246E-4 0.0 0.0 0.0 10 0.0 1.7718307263797246E-4 0.0 0.0 0.0 11 0.0 1.7718307263797246E-4 0.0 0.0 0.0 12 0.0 1.7718307263797246E-4 0.0 0.0 0.0 13 0.0 1.7718307263797246E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAACC 40 0.0052148905 14.274054 3 TCCTAGA 120 4.9112714E-11 13.490639 2 TAGGACC 265 0.0 13.286541 4 GACGTGG 250 0.0 12.909695 7 AGGACCC 60 4.0271165E-4 12.688048 5 GTATAGG 85 3.8115359E-6 12.328012 1 CGTGGAA 270 0.0 12.302813 9 GTTCAAG 70 1.0608928E-4 12.247961 1 TAAGCCT 55 0.0030258026 12.111319 4 GCGCCAC 55 0.0030887923 12.078054 13 ACGTGGA 270 0.0 11.9513035 8 GTAGGAC 575 0.0 11.915732 3 TGTAGGG 65 7.8499713E-4 11.720374 2 TAACACC 65 7.8969524E-4 11.712044 4 CGCCACC 65 8.0813287E-4 11.679877 14 GTATTAG 75 2.0170811E-4 11.431431 1 CTGTAGG 620 0.0 11.369971 1 TGTAGGA 640 0.0 11.30833 2 GTCCTAC 515 0.0 11.28345 1 GGCGAGA 345 0.0 11.28085 19 >>END_MODULE