FastQCFastQC Report
Thu 26 May 2016
SRR1926992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356172
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10530.2956436777736599No Hit
GTCCTACAGTGGACATTTCTAAATT6540.18361915029817052No Hit
GGTATCAACGCAGAGTACTTTTTTT6320.17744235930954708No Hit
GTCCTACAGTGTGCATTTCTCATTT5770.1620003818379884No Hit
TATCAACGCAGAGTACTTTTTTTTT5520.15498130116909808No Hit
CTGTAGGACGTGGAATATGGCAAGA5040.1415046662848287No Hit
GTCCTAAAGTGTGTATTTCTCATTT4430.12437810945273632No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG3910.10977842166144448No Hit
GTACATGGGAAGCAGTGGTATCAAC3630.10191705131228732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCA307.781473E-418.9727639
GGGCACT350.00218663716.2623711
GTTCTAG400.00514301514.3018561
ACTGATC400.005245017414.2596038
TAGGACC1600.013.6808494
GTTCTAA500.001455181613.3483981
CTAGGAC500.001464497213.3371083
TAACACC500.001475429913.32395654
GTAGCCC1153.5652192E-1013.2542683
CTGGAGT655.338498E-513.1775414
GTTCAGA603.955109E-412.7127611
CTTGTAC603.9834657E-412.7020063
TGTAGCC1207.003109E-1012.7020062
GTATATC1155.0513336E-912.4258773
TGTAGGA3550.012.3442032
CCTACAC701.0584377E-412.24836353
GGCTAAG550.002978272712.1349071
CTACACC550.00301945912.1126894
GCACAGT550.003029032812.1075736
GACGTGG1501.2732926E-1112.0499187