##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926992_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356172 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.158886717653267 31.0 28.0 31.0 16.0 34.0 2 28.290019990341744 31.0 28.0 31.0 16.0 34.0 3 28.056012263737745 31.0 28.0 33.0 16.0 34.0 4 31.6473052345496 35.0 32.0 37.0 19.0 37.0 5 31.463792774277596 35.0 32.0 37.0 17.0 37.0 6 31.192418269824692 35.0 32.0 37.0 16.0 37.0 7 30.990016059656572 35.0 32.0 37.0 12.0 37.0 8 30.812132902081018 35.0 32.0 37.0 11.0 37.0 9 32.94530170816347 37.0 33.0 39.0 17.0 39.0 10 33.1638253428119 37.0 33.0 39.0 17.0 39.0 11 33.307289736419484 38.0 34.0 39.0 17.0 39.0 12 33.20467358467258 38.0 33.0 39.0 17.0 39.0 13 33.2800023584111 38.0 34.0 39.0 17.0 39.0 14 34.27306750671024 39.0 34.0 41.0 17.0 41.0 15 33.89523320193614 39.0 33.0 41.0 11.0 41.0 16 33.959957548600116 39.0 33.0 41.0 15.0 41.0 17 34.195456127938186 39.0 34.0 41.0 17.0 41.0 18 34.236950125220396 39.0 34.0 41.0 17.0 41.0 19 33.65787597003695 39.0 33.0 41.0 10.0 41.0 20 33.95577417652146 39.0 34.0 41.0 10.0 41.0 21 33.940860033916195 39.0 34.0 41.0 10.0 41.0 22 33.94999887694709 39.0 34.0 41.0 2.0 41.0 23 33.93955729254405 39.0 34.0 41.0 2.0 41.0 24 32.683133429915884 39.0 32.0 40.0 2.0 41.0 25 33.23635209954741 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.22862069705920973 1101 2 -0.23238130053905337 1101 3 -0.7307074568230796 1101 4 -0.4367979158517059 1101 5 -0.34219473345212137 1101 6 -2.304124783157963 1101 7 -0.6898823415871931 1101 8 -0.3066366171343873 1101 9 -0.33323869725570887 1101 10 -8.487026237153248 1101 11 -3.8709763763335587 1101 12 -1.748680444102213 1101 13 -1.1863995508152527 1101 14 -0.9250375807334095 1101 15 -2.262468978304188 1101 16 -0.7597466212029858 1101 17 -0.7195648443006633 1101 18 -0.690215407163862 1101 19 -1.1692467905813544 1101 20 -0.43662342155519696 1101 21 -0.49256312100891364 1101 22 -0.26024757142941013 1101 23 -0.17585454392251165 1101 24 -0.598680824183333 1101 25 -0.4205897090189694 1102 1 -0.09749787154524014 1102 2 0.13316078345321358 1102 3 -0.06644809994379486 1102 4 -0.12629361080865564 1102 5 -0.09843089581374542 1102 6 -0.9439596100776626 1102 7 -0.29086283710643457 1102 8 0.2868162031080992 1102 9 0.4448705981229608 1102 10 0.29257664458985744 1102 11 0.3815170343148324 1102 12 0.30804069453774474 1102 13 -0.11347387870138448 1102 14 0.07559499702094286 1102 15 0.3903442355422371 1102 16 0.19396977310381658 1102 17 0.13263687771655697 1102 18 0.01919594409250891 1102 19 0.415392112950542 1102 20 0.3067151832594206 1102 21 0.19564281819333473 1102 22 0.19870516095505764 1102 23 0.18977188377034082 1102 24 -3.0046867985287946 1102 25 -1.5372792187712143 1103 1 0.15143205784096025 1103 2 -0.1870275200050493 1103 3 0.2283373427503932 1103 4 0.022857213018973255 1103 5 0.047272526239488855 1103 6 0.45100689363409074 1103 7 0.08240169304022515 1103 8 0.031878669159159045 1103 9 -0.05968489707892388 1103 10 -0.16454086401054724 1103 11 -0.24148133613601175 1103 12 -0.32031346081260637 1103 13 -0.13657002210170788 1103 14 -0.5105538351354966 1103 15 -0.07217251260188817 1103 16 -0.4109188743703811 1103 17 -0.11326933267634587 1103 18 -0.14100322302680723 1103 19 -1.672158131481833 1103 20 -0.6425008225345294 1103 21 -0.6715018495596965 1103 22 -0.42707792794590205 1103 23 -0.6659840459779858 1103 24 1.08914626321301 1103 25 0.21235684858348236 1104 1 0.04673453783642145 1104 2 0.3633668957488787 1104 3 -0.6081858398158637 1104 4 -0.06475132225144264 1104 5 -0.18044582118687913 1104 6 -1.206504030831038 1104 7 -0.03954087548596519 1104 8 0.7288524504428544 1104 9 0.6587547487981595 1104 10 0.8580954789892843 1104 11 0.5034040279182435 1104 12 0.5717400548788945 1104 13 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-0.55651650084625 2214 22 -0.13857902759389162 2214 23 -0.5179483442871557 2214 24 -1.7918352768071202 2214 25 -0.8998713652419426 2215 1 -0.11798213304645344 2215 2 -0.758768191526606 2215 3 -0.09405100551280299 2215 4 0.16263294286365948 2215 5 0.07885723977947379 2215 6 0.5694382914558318 2215 7 0.2886529013923571 2215 8 -0.6238859760686566 2215 9 -0.2564046238302282 2215 10 -0.6291989809145875 2215 11 -0.6071512465568389 2215 12 -1.274041629917459 2215 13 -0.8247571716795932 2215 14 -0.8980127511630798 2215 15 -0.7578720679283037 2215 16 -0.3672893067993286 2215 17 -0.7191145347128156 2215 18 -1.5218371471100767 2215 19 -0.6902737466136273 2215 20 -4.514802169444373 2215 21 -3.2749947927832572 2215 22 -1.998792821288692 2215 23 -1.705643774018199 2215 24 -1.8349370003044179 2215 25 -1.645656942742157 2216 1 -0.14233699638436548 2216 2 -0.5615625112700791 2216 3 0.09880286530717086 2216 4 -0.16124856576361069 2216 5 0.1307582933754432 2216 6 -2.027614693732744 2216 7 -10.092458718702314 2216 8 -11.208524852232955 2216 9 -5.812581139328778 2216 10 -3.5458274738142634 2216 11 -2.32103122823343 2216 12 -1.8368756503785981 2216 13 -1.6133966586241648 2216 14 -1.5179699965440534 2216 15 -2.7349067297087295 2216 16 -1.6531344740004315 2216 17 -2.026642401562718 2216 18 -1.8149353300387645 2216 19 -1.1153157377573137 2216 20 -2.07459755805332 2216 21 -4.92965962710911 2216 22 -3.051487889237997 2216 23 -3.204513830515374 2216 24 -2.8737215475635445 2216 25 -2.4251041865961795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21111.0 3 1.0 4 151.0 5 1002.0 6 1516.0 7 1132.0 8 967.0 9 799.0 10 697.0 11 715.0 12 654.0 13 678.0 14 649.0 15 672.0 16 698.0 17 732.0 18 895.0 19 1131.0 20 1477.0 21 1894.0 22 2264.0 23 2718.0 24 3260.0 25 3792.0 26 4304.0 27 4688.0 28 5002.0 29 6144.0 30 7202.0 31 8988.0 32 11440.0 33 14927.0 34 20182.0 35 28229.0 36 41242.0 37 65551.0 38 88668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85576947424394 18.828971023911816 12.590348056303474 24.724911445540766 2 13.779823064575528 19.947543453754747 43.635356724192704 22.637276757477025 3 19.419642857142858 24.88530046779417 29.260188017272398 26.434868657790574 4 10.879178302326757 17.233512948593326 39.54476240778095 32.34254634129896 5 11.916763784945028 39.72338842162906 35.683736081663604 12.676111711762312 6 31.049230691465013 37.702998323499926 19.01634096136187 12.231430023673193 7 27.47038021443373 32.70424137309668 21.70859987386251 18.116778538607083 8 24.02799596840037 38.46376909519868 20.67799568686408 16.830239249536874 9 26.310542750595317 16.24348519566878 20.290245765377186 37.15572628835872 10 13.50227916787202 31.020275185296487 35.3272424456047 20.15020320122679 11 33.85525624861352 23.782218865042303 23.85803621823042 18.504488668113748 12 22.414170743076657 26.487023818215516 32.2657912915527 18.833014147155126 13 28.55678671915804 22.98678542746587 25.76953965214168 22.68688820123441 14 19.514482681156366 23.83892843603892 28.735099616696854 27.911489266107857 15 22.73337376868731 32.32104547854118 23.54657366535252 21.39900708741899 16 20.30788412504072 30.18887528502589 29.40343940602289 20.099801183910497 17 20.409137497718433 30.109093972452715 28.434634879182287 21.04713365064656 18 19.634453167997666 29.54701171502061 30.7201985825162 20.098336534465524 19 22.313896408793234 28.228940835514415 28.67320804735855 20.783954708333802 20 21.9940187860663 28.472263173413083 29.912247448155792 19.621470592364826 21 22.103723172741848 27.941626581976355 28.199684938546167 21.75496530673563 22 21.40975743994968 29.65196929107768 28.847460673147662 20.090812595824982 23 20.967664939696444 28.987826966008175 28.891790573269873 21.15271752102551 24 21.51824801120967 28.22186840989439 29.946563106359918 20.31332047253602 25 21.265626579506012 28.478282845301074 29.545607709674158 20.71048286551875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 139.0 2 195.0 3 251.0 4 251.0 5 251.0 6 395.5 7 540.0 8 540.0 9 540.0 10 768.0 11 996.0 12 996.0 13 996.0 14 1639.0 15 2282.0 16 2282.0 17 2282.0 18 3663.5 19 5045.0 20 5045.0 21 5045.0 22 7727.5 23 10410.0 24 10410.0 25 10410.0 26 14609.0 27 18808.0 28 18808.0 29 18808.0 30 24270.5 31 29733.0 32 29733.0 33 29733.0 34 33949.0 35 38165.0 36 38165.0 37 38165.0 38 41123.0 39 44081.0 40 44081.0 41 44081.0 42 46034.0 43 47987.0 44 47987.0 45 47987.0 46 48593.5 47 49200.0 48 49200.0 49 49200.0 50 46220.0 51 43240.0 52 43240.0 53 43240.0 54 36378.5 55 29517.0 56 29517.0 57 29517.0 58 24032.0 59 18547.0 60 18547.0 61 18547.0 62 14241.5 63 9936.0 64 9936.0 65 9936.0 66 7240.5 67 4545.0 68 4545.0 69 4545.0 70 3182.5 71 1820.0 72 1820.0 73 1820.0 74 1234.0 75 648.0 76 648.0 77 648.0 78 424.5 79 201.0 80 201.0 81 201.0 82 131.5 83 62.0 84 62.0 85 62.0 86 36.0 87 10.0 88 10.0 89 10.0 90 5.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 1.0 98 4.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20804555102590883 2 0.12606268881326999 3 0.12915108430758174 4 0.06345248924676843 5 0.02161876846018216 6 0.02049571555315971 7 0.2818862796626349 8 0.274586435766989 9 0.016845793605336747 10 0.033972350437429105 11 0.01488045101804746 12 0.016284267151825522 13 0.014038161337780623 14 0.01600350392506991 15 0.013476634884269399 16 0.017968846512359197 17 0.01600350392506991 18 0.013476634884269399 19 0.022461058140448995 20 0.01600350392506991 21 0.014318924564536234 22 0.015161214244803071 23 0.01600350392506991 24 0.014318924564536234 25 0.013476634884269399 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 356172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.50467124798148 #Duplication Level Percentage of deduplicated Percentage of total 1 96.31971570293416 86.21064488690162 2 2.80261654855035 5.0169454562430325 3 0.35940383715911794 0.9650496687056977 4 0.13388023912730343 0.47931627158761764 5 0.08790929590849994 0.3934146314965905 6 0.060645414787536885 0.3256828747953578 7 0.03720773785013195 0.23311864409099964 8 0.024285388896455495 0.17389245994453031 9 0.020594276075202318 0.16589555187310384 >10 0.12670405606044147 2.240606088173409 >50 0.014776309594030153 0.9280352593235919 >100 0.010374855663452984 1.7497038322162057 >500 0.0015719478277959067 0.8213871801606795 >1k 3.143895655591813E-4 0.2963071944875627 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1053 0.2956436777736599 No Hit GTCCTACAGTGGACATTTCTAAATT 654 0.18361915029817052 No Hit GGTATCAACGCAGAGTACTTTTTTT 632 0.17744235930954708 No Hit GTCCTACAGTGTGCATTTCTCATTT 577 0.1620003818379884 No Hit TATCAACGCAGAGTACTTTTTTTTT 552 0.15498130116909808 No Hit CTGTAGGACGTGGAATATGGCAAGA 504 0.1415046662848287 No Hit GTCCTAAAGTGTGTATTTCTCATTT 443 0.12437810945273632 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 391 0.10977842166144448 No Hit GTACATGGGAAGCAGTGGTATCAAC 363 0.10191705131228732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.8076322675561245E-4 13 0.0 0.0 0.0 0.0 2.8076322675561245E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGATCA 30 7.781473E-4 18.972763 9 GGGCACT 35 0.002186637 16.26237 11 GTTCTAG 40 0.005143015 14.301856 1 ACTGATC 40 0.0052450174 14.259603 8 TAGGACC 160 0.0 13.680849 4 GTTCTAA 50 0.0014551816 13.348398 1 CTAGGAC 50 0.0014644972 13.337108 3 TAACACC 50 0.0014754299 13.3239565 4 GTAGCCC 115 3.5652192E-10 13.254268 3 CTGGAGT 65 5.338498E-5 13.177541 4 GTTCAGA 60 3.955109E-4 12.712761 1 CTTGTAC 60 3.9834657E-4 12.702006 3 TGTAGCC 120 7.003109E-10 12.702006 2 GTATATC 115 5.0513336E-9 12.425877 3 TGTAGGA 355 0.0 12.344203 2 CCTACAC 70 1.0584377E-4 12.2483635 3 GGCTAAG 55 0.0029782727 12.134907 1 CTACACC 55 0.003019459 12.112689 4 GCACAGT 55 0.0030290328 12.107573 6 GACGTGG 150 1.2732926E-11 12.049918 7 >>END_MODULE