FastQCFastQC Report
Thu 26 May 2016
SRR1926992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356172
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16720.469436115135384No Hit
GGTATCAACGCAGAGTACTTTTTTT12190.34225037341509157No Hit
TATCAACGCAGAGTACTTTTTTTTT9050.2540907202138293No Hit
GTCCTACAGTGGACATTTCTAAATT6350.17828464898981392No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6130.17210785800119044No Hit
CTGTAGGACGTGGAATATGGCAAGA5860.1645272508787889No Hit
GTCCTACAGTGTGCATTTCTCATTT5430.15245443212829757No Hit
CACACACACACACACACACACACAC5370.1507698527677639No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG4640.13027413721460418No Hit
GTACATGGGAAGCAGTGGTATCAAC4540.12746650494704806No Hit
GTCCTAAAGTGTGTATTTCTCATTT4250.11932437137113529No Hit
CTTTAGGACGTGAAATATGGCGAGG4040.11342834360926744No Hit
CTGTAGGACCTGGAATATGGCGAGA3860.10837460552766641No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC551.0945301E-515.58824353
TAGGACC1203.6379788E-1214.28720954
CTGATCA400.00531526114.2310479
AGTGTCC400.00531526114.2310478
GGCGAGG852.7230817E-713.39392619
AAACAGC500.001466467713.3347286
GTGTAGC1153.5470293E-1013.259381
AGAACTG655.2981497E-513.1881945
GTATAAG603.9705224E-412.7069051
CCTGAAG603.984725E-412.7015313
TAGACAG751.4320654E-512.6997415
TAATACA701.0602031E-412.246184
TGCACAG701.0602031E-412.246185
GTCCTAC3400.012.0528741
CCCTGTA802.760683E-511.9127242
TAGAGTG657.823616E-411.7228395
CTACTCT657.823616E-411.7228394
TCAGATG658.0865866E-411.67675714
TGTAGCC1406.366463E-1011.572362
TGGCGAG2450.011.22994218