##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356172 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.7959777860135 31.0 30.0 33.0 26.0 34.0 2 30.142453645991264 31.0 30.0 33.0 26.0 34.0 3 30.204684815201645 31.0 30.0 34.0 26.0 34.0 4 33.792280134317124 35.0 35.0 37.0 28.0 37.0 5 33.75430690789843 37.0 35.0 37.0 28.0 37.0 6 33.703730220230675 37.0 35.0 37.0 29.0 37.0 7 33.70009433644419 37.0 35.0 37.0 29.0 37.0 8 33.73412283952697 37.0 35.0 37.0 29.0 37.0 9 35.24507822063497 39.0 35.0 39.0 29.0 39.0 10 35.13593151623373 39.0 35.0 39.0 29.0 39.0 11 35.180878901205034 39.0 35.0 39.0 29.0 39.0 12 35.058561593836686 39.0 35.0 39.0 28.0 39.0 13 35.07492728232427 39.0 35.0 39.0 29.0 39.0 14 36.143587929427355 40.0 36.0 41.0 28.0 41.0 15 36.1393175207484 40.0 36.0 41.0 28.0 41.0 16 36.10876205878059 40.0 36.0 41.0 28.0 41.0 17 36.107495816627925 40.0 36.0 41.0 28.0 41.0 18 36.09561391687162 40.0 36.0 41.0 28.0 41.0 19 36.1797053109172 40.0 37.0 41.0 28.0 41.0 20 36.147372617724024 40.0 37.0 41.0 28.0 41.0 21 36.0965853576362 40.0 37.0 41.0 28.0 41.0 22 36.00161719618611 40.0 36.0 41.0 27.0 41.0 23 35.93418067675168 40.0 36.0 41.0 27.0 41.0 24 35.86501184820817 40.0 36.0 41.0 27.0 41.0 25 35.7578922543041 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04187302905821255 1101 2 0.1786847337100177 1101 3 0.3793281959192072 1101 4 0.053755705175326796 1101 5 0.02830949232545521 1101 6 0.10193836779217946 1101 7 -0.1435252832346947 1101 8 0.17032655407457042 1101 9 0.3258879023197778 1101 10 0.6071586400495477 1101 11 0.28171891235049173 1101 12 0.5456469962539501 1101 13 0.2107647039923819 1101 14 0.2933634916688561 1101 15 0.2765864907916935 1101 16 0.5341393245483701 1101 17 0.5323044382384694 1101 18 0.5227320249882226 1101 19 0.4922183560214677 1101 20 0.3622733553161481 1101 21 0.44837798162644305 1101 22 0.4165355728807185 1101 23 0.46745480776899484 1101 24 0.2095421939821449 1101 25 0.0786376637452122 1102 1 -1.2136180972816213 1102 2 -1.055379578361677 1102 3 -0.9285157682347212 1102 4 -0.9810817574850148 1102 5 -0.961238917444355 1102 6 -0.8561779562282013 1102 7 -0.7952420289735826 1102 8 -1.0603358954070714 1102 9 -0.8681482951850583 1102 10 -0.8001300614089004 1102 11 -1.0483390739436516 1102 12 -0.987907739293405 1102 13 -1.0113403742012608 1102 14 -1.198008850625996 1102 15 -1.523476766983741 1102 16 -1.1166144972755632 1102 17 -0.9035697490748333 1102 18 -1.0262997796155418 1102 19 -1.2209076323812695 1102 20 -1.2467092487956037 1102 21 -1.1851802682417656 1102 22 -1.261443692626223 1102 23 -1.160290755400922 1102 24 -0.9046697195655611 1102 25 -1.0489954411572668 1103 1 -0.9201181878121325 1103 2 -1.067025423011323 1103 3 -0.7218672076698098 1103 4 -0.7564248681132995 1103 5 -0.8829191636873404 1103 6 -0.7247421423589273 1103 7 -0.6449143085317957 1103 8 -0.7803069256442612 1103 9 -0.8213838555689037 1103 10 -0.8160380824119784 1103 11 -0.9194091445574486 1103 12 -0.8740420865684371 1103 13 -1.0647533743823203 1103 14 -0.7816228285365554 1103 15 -1.0988923020190455 1103 16 -0.9854683810343872 1103 17 -0.8412521680500333 1103 18 -0.7583982768090962 1103 19 -0.8160189849070747 1103 20 -0.9214946914897908 1103 21 -1.1680265474378544 1103 22 -1.0120067923908422 1103 23 -0.9679134242403222 1103 24 -0.8077224083216663 1103 25 -0.731037809435378 1104 1 0.09830443292448976 1104 2 0.16592106129717266 1104 3 0.4234778351767936 1104 4 0.33769578338208106 1104 5 0.39726499158194173 1104 6 0.3835377509501896 1104 7 0.7584822069894628 1104 8 0.5464693640101288 1104 9 0.4538275399251077 1104 10 0.33411656261384337 1104 11 0.5990276637962211 1104 12 0.6087838029951484 1104 13 0.09118272459049592 1104 14 0.5579790669290858 1104 15 0.4795935954896109 1104 16 0.612511302297996 1104 17 0.54152264609219 1104 18 0.49809965179881743 1104 19 0.6647000435249097 1104 20 0.6780881925689997 1104 21 0.7630500089437362 1104 22 1.030052398347479 1104 23 0.9219764798513879 1104 24 1.0653622633768691 1104 25 0.4392561579898526 1105 1 0.07909898955310979 1105 2 0.1497884888652159 1105 3 0.39546532420225233 1105 4 0.5009420520387948 1105 5 0.3195103822755314 1105 6 0.1687660213628348 1105 7 -0.11256695894557112 1105 8 -0.0665683884042707 1105 9 0.08656458996900795 1105 10 0.10063324391322936 1105 11 0.23203907731684836 1105 12 0.28594029992228087 1105 13 0.05334800114887628 1105 14 0.3706711213627969 1105 15 0.4255988632683625 1105 16 0.516977939699224 1105 17 0.32033081114925466 1105 18 0.4670965789278796 1105 19 0.38103564492965347 1105 20 0.8823243646497687 1105 21 0.6078974628506728 1105 22 0.7677334782421283 1105 23 0.7213619056634997 1105 24 0.6337336417789814 1105 25 0.5165717857334897 1106 1 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1107 13 -0.134847020676105 1107 14 -0.02878455313314987 1107 15 -0.3015888315975701 1107 16 0.09239105547557358 1107 17 -0.1192217766703294 1107 18 -0.19314817268301 1107 19 -0.10283595141206803 1107 20 -0.2792584663624922 1107 21 -0.1252206518863943 1107 22 -0.19190978719125695 1107 23 -0.2365990172215504 1107 24 -0.28847587807136676 1107 25 -0.4993942296479261 1108 1 0.18334851854620382 1108 2 0.12139723912688538 1108 3 0.23837797858785237 1108 4 0.2560577104017838 1108 5 0.2100405267176484 1108 6 0.27728077957928576 1108 7 -0.07161552499120916 1108 8 0.08187728879899936 1108 9 -0.010266580221490074 1108 10 -0.17130782788915155 1108 11 -0.019452444558801574 1108 12 -0.06966337393160416 1108 13 -0.23647170787351968 1108 14 0.03634125952482492 1108 15 -0.1422261314982265 1108 16 -0.08393529036148095 1108 17 -0.04623031508059938 1108 18 -0.05672852050691546 1108 19 0.08859923086224342 1108 20 -0.0948841344082112 1108 21 0.23091530877907473 1108 22 0.12030177424379929 1108 23 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0.260275516999279 2214 8 0.369809451095783 2214 9 0.5646855601108598 2214 10 0.06327955876295022 2214 11 0.06897241726328218 2214 12 -0.040611505454172914 2214 13 0.3234383939670522 2214 14 0.34886338899497815 2214 15 0.45550661051566266 2214 16 0.2844551245023226 2214 17 0.6275857957737969 2214 18 0.32496658190377303 2214 19 0.6527131818803511 2214 20 0.5126065787158396 2214 21 0.6167662506693361 2214 22 0.6792966912170257 2214 23 0.6406407470482876 2214 24 0.7523478665582672 2214 25 0.7346615089752291 2215 1 0.0029275040097402893 2215 2 -0.10312776238104604 2215 3 -0.24493223312576262 2215 4 -0.11494777846967708 2215 5 0.12817189772916748 2215 6 0.029907596637009703 2215 7 -0.23642039862652808 2215 8 -0.13628424084694046 2215 9 -0.30395136217456553 2215 10 -0.11181690563811486 2215 11 -0.47695892866520495 2215 12 -0.4369472873160092 2215 13 -0.443866605032575 2215 14 -0.3179442824258345 2215 15 -0.05137022945346814 2215 16 -0.4083336256048611 2215 17 -0.2957085707051803 2215 18 -0.5829906593572645 2215 19 -0.322360416884905 2215 20 -0.44317413983677056 2215 21 -0.2131705830117454 2215 22 -0.43899010102008873 2215 23 -0.2511704971281219 2215 24 -0.012078920534079884 2215 25 -0.04226420629044014 2216 1 0.248415364751839 2216 2 0.3035908206087221 2216 3 -1.4043458840648348 2216 4 -1.2115579479612038 2216 5 -0.13802141422685565 2216 6 0.268249328199083 2216 7 0.3690308152992614 2216 8 0.13760490253373092 2216 9 0.22904604200927992 2216 10 0.17639503511620092 2216 11 0.046281260676678926 2216 12 0.3829030715174042 2216 13 0.2123404494795622 2216 14 0.08950723689009266 2216 15 0.04398784048086668 2216 16 0.12570210504103585 2216 17 0.06466705916196958 2216 18 0.425025831710073 2216 19 -0.00983942131306037 2216 20 0.05218393009756994 2216 21 0.15299218993069275 2216 22 -0.09727380845633604 2216 23 -0.09954429465446424 2216 24 0.0031295082336697533 2216 25 -0.19247034463217005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8687.0 3 6.0 4 138.0 5 945.0 6 1364.0 7 1048.0 8 821.0 9 703.0 10 614.0 11 532.0 12 521.0 13 458.0 14 443.0 15 474.0 16 457.0 17 460.0 18 540.0 19 631.0 20 867.0 21 1128.0 22 1381.0 23 1666.0 24 2044.0 25 2396.0 26 2739.0 27 3101.0 28 3579.0 29 4399.0 30 5547.0 31 7460.0 32 9341.0 33 12493.0 34 16980.0 35 23867.0 36 35353.0 37 61473.0 38 141516.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.224932828186304 19.62709806522692 10.925959235983857 27.222009870602914 2 13.64519129967988 20.919722430670905 41.776769729316506 23.658316540332713 3 18.981043385964075 24.887330152837876 28.866506237908172 27.265120223289873 4 11.291173927203712 17.623507743449792 38.67738059139966 32.40793773794684 5 12.175981041802377 39.43316336844943 35.24366001033267 13.14719557941553 6 30.76790910038335 38.54614693447578 18.197668359486126 12.48827560565475 7 28.174144051042415 32.41401195183322 20.905036843997124 18.506807153127248 8 24.79159003675428 39.07971101982597 19.438212658185197 16.690486285234552 9 26.218240118602044 16.24460548815243 19.977874428960913 37.559279964284606 10 13.865639415882145 31.473125917773142 34.345141663461185 20.316093002883527 11 33.817575345773506 23.770349114718748 23.514844535290518 18.89723100421723 12 22.576171063418798 26.771054434374403 31.70687196073807 18.94590254146873 13 28.760342644403952 22.974638586521642 25.616908732041633 22.648110037032772 14 19.924137563136807 23.915198036898005 28.511024199050457 27.649640200914728 15 22.652332365608906 32.08455702742011 23.63362739427564 21.629483212695348 16 20.51817661130016 30.24409554934133 28.99554147995912 20.24218635939939 17 20.215513852857608 30.091921880439788 28.374773985602463 21.317790281100145 18 19.631581459467505 29.428561000193724 30.74534423072064 20.194513309618134 19 21.865840099727098 28.207719865682872 29.060959311793177 20.86548072279685 20 22.065305893253225 28.458320464946514 29.869444366454225 19.606929275346044 21 22.01265278959472 27.639578015707876 28.628831849312185 21.718937345385214 22 21.436553126017767 29.53376458564963 28.75576968430983 20.273912604022776 23 20.919106499107173 28.612299675437708 29.092685556416566 21.375908269038554 24 21.047976820188 28.038138876722485 30.46084476039666 20.453039542692856 25 21.046454493127992 28.497143097808536 29.63878086788056 20.817621541182913 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 201.0 1 201.0 2 197.0 3 193.0 4 193.0 5 193.0 6 322.0 7 451.0 8 451.0 9 451.0 10 730.0 11 1009.0 12 1009.0 13 1009.0 14 1465.0 15 1921.0 16 1921.0 17 1921.0 18 3245.5 19 4570.0 20 4570.0 21 4570.0 22 7172.5 23 9775.0 24 9775.0 25 9775.0 26 14030.0 27 18285.0 28 18285.0 29 18285.0 30 23801.0 31 29317.0 32 29317.0 33 29317.0 34 33307.0 35 37297.0 36 37297.0 37 37297.0 38 40406.0 39 43515.0 40 43515.0 41 43515.0 42 45707.0 43 47899.0 44 47899.0 45 47899.0 46 48966.0 47 50033.0 48 50033.0 49 50033.0 50 47220.0 51 44407.0 52 44407.0 53 44407.0 54 37318.5 55 30230.0 56 30230.0 57 30230.0 58 24733.5 59 19237.0 60 19237.0 61 19237.0 62 14736.0 63 10235.0 64 10235.0 65 10235.0 66 7507.5 67 4780.0 68 4780.0 69 4780.0 70 3329.0 71 1878.0 72 1878.0 73 1878.0 74 1253.5 75 629.0 76 629.0 77 629.0 78 419.0 79 209.0 80 209.0 81 209.0 82 144.0 83 79.0 84 79.0 85 79.0 86 49.5 87 20.0 88 20.0 89 20.0 90 11.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10332086744606539 2 0.103601630672821 3 0.012072818750491335 4 0.0 5 0.005615264535112249 6 0.320912368181665 7 0.021338005233426546 8 0.006457554215379087 9 0.006457554215379087 10 0.003088395494311737 11 0.003930685174578574 12 0.0 13 2.8076322675561245E-4 14 2.8076322675561245E-4 15 0.0028076322675561244 16 0.0 17 0.0 18 2.8076322675561245E-4 19 0.0 20 5.615264535112249E-4 21 0.012634345204002561 22 0.0 23 0.0 24 0.0 25 0.0047729748548454125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 356172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.36808919092479 #Duplication Level Percentage of deduplicated Percentage of total 1 95.56798684647403 83.49592398599872 2 3.398375564909579 5.93819158918559 3 0.4177236964589176 1.094871635081565 4 0.17358660308877097 0.6066371928403761 5 0.10490674015401662 0.4582750715252648 6 0.060228741185716354 0.31572420190624756 7 0.049003342268874116 0.2996929864600287 8 0.03322465496794997 0.2322219694862044 9 0.023336895537982138 0.18350099737215467 >10 0.14012710194595643 2.470363379759979 >50 0.017035613163815737 1.0393308600181772 >100 0.011892786538731595 1.98093485059571 >500 0.001928559979253772 1.0724681307662343 >1k 6.42853326417924E-4 0.8118631490037139 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1672 0.469436115135384 No Hit GGTATCAACGCAGAGTACTTTTTTT 1219 0.34225037341509157 No Hit TATCAACGCAGAGTACTTTTTTTTT 905 0.2540907202138293 No Hit GTCCTACAGTGGACATTTCTAAATT 635 0.17828464898981392 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 613 0.17210785800119044 No Hit CTGTAGGACGTGGAATATGGCAAGA 586 0.1645272508787889 No Hit GTCCTACAGTGTGCATTTCTCATTT 543 0.15245443212829757 No Hit CACACACACACACACACACACACAC 537 0.1507698527677639 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 464 0.13027413721460418 No Hit GTACATGGGAAGCAGTGGTATCAAC 454 0.12746650494704806 No Hit GTCCTAAAGTGTGTATTTCTCATTT 425 0.11932437137113529 No Hit CTTTAGGACGTGAAATATGGCGAGG 404 0.11342834360926744 No Hit CTGTAGGACCTGGAATATGGCGAGA 386 0.10837460552766641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGGAC 55 1.0945301E-5 15.5882435 3 TAGGACC 120 3.6379788E-12 14.2872095 4 CTGATCA 40 0.005315261 14.231047 9 AGTGTCC 40 0.005315261 14.231047 8 GGCGAGG 85 2.7230817E-7 13.393926 19 AAACAGC 50 0.0014664677 13.334728 6 GTGTAGC 115 3.5470293E-10 13.25938 1 AGAACTG 65 5.2981497E-5 13.188194 5 GTATAAG 60 3.9705224E-4 12.706905 1 CCTGAAG 60 3.984725E-4 12.701531 3 TAGACAG 75 1.4320654E-5 12.699741 5 TAATACA 70 1.0602031E-4 12.24618 4 TGCACAG 70 1.0602031E-4 12.24618 5 GTCCTAC 340 0.0 12.052874 1 CCCTGTA 80 2.760683E-5 11.912724 2 TAGAGTG 65 7.823616E-4 11.722839 5 CTACTCT 65 7.823616E-4 11.722839 4 TCAGATG 65 8.0865866E-4 11.676757 14 TGTAGCC 140 6.366463E-10 11.57236 2 TGGCGAG 245 0.0 11.229942 18 >>END_MODULE