FastQCFastQC Report
Thu 26 May 2016
SRR1926991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18498
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC5132.7732727862471616No Hit
CCCATGTACTCTGCGTTGATACCAC4322.335387609471294No Hit
GAGTACATGGGAAGCAGTGGTATCA2511.356903449021516No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA1861.0055141096334739No Hit
GCGTTGATACCACTGCTTCCCATGT1680.9082062925721699No Hit
CATGTACTCTGCGTTGATACCACTG1640.8865823332252135No Hit
GGTATCAACGCAGAGTACATGGGAA1530.8271164450210833No Hit
ACGCAGAGTACATGGGAAGCAGTGG1380.7460265974699967No Hit
TATCAACGCAGAGTACATGGGAAGC1370.7406206076332577No Hit
GTATCAACGCAGAGTACTTTTTTTT1330.7189966482863013No Hit
ACTCTGCGTTGATACCACTGCTTCC1230.6649367499189102No Hit
GTATCAACGCAGAGTACATGGGAAG1130.610876851551519No Hit
GGTATCAACGCAGAGTACTTTTTTT1040.5622229430208671No Hit
GTGGTATCAACGCAGAGTACATGGG910.49194507514325875No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA880.4757271056330414No Hit
GCTTCCCATGTACTCTGCGTTGATA780.4216672072656503No Hit
TATCAACGCAGAGTACTTTTTTTTT780.4216672072656503No Hit
GCAGTGGTATCAACGCAGAGTACAT710.3838252784084766No Hit
GTACTCTGCGTTGATACCACTGCTT710.3838252784084766No Hit
CAGTGGTATCAACGCAGAGTACATG620.33517136987782464No Hit
GTTGATACCACTGCTTCCCATGTAC580.3135474105308682No Hit
CATGGGAAGCAGTGGTATCAACGCA570.3081414206941291No Hit
ATACCACTGCTTCCCATGTACTCTG570.3081414206941291No Hit
GATACCACTGCTTCCCATGTACTCT550.2973294410206509No Hit
CTGCTTCCCATGTACTCTGCGTTGA550.2973294410206509No Hit
ACATGGGAAGCAGTGGTATCAACGC540.2919234511839118No Hit
GCAGAGTACATGGGAAGCAGTGGTA510.27570548167369446No Hit
TACCACTGCTTCCCATGTACTCTGC500.27029949183695534No Hit
CTTCCCATGTACTCTGCGTTGATAC490.2648935020002163No Hit
CCACTGCTTCCCATGTACTCTGCGT440.2378635528165207No Hit
ACGCAGAGTACTTTTTTTTTTTTTT440.2378635528165207No Hit
GGGAAGCAGTGGTATCAACGCAGAG420.22705157314304247No Hit
ATGGGAAGCAGTGGTATCAACGCAG400.21623959346956426No Hit
AAGCAGTGGTATCAACGCAGAGTAC370.20002162395934697No Hit
GTACTGGTTCACTATCGGTCAGTCA310.16758568493891232No Hit
TTCCCATGTACTCTGCGTTGATACC310.16758568493891232No Hit
CCATGTACTCTGCGTTGATACCACT300.1621796951021732No Hit
TGATACCACTGCTTCCCATGTACTC280.15136771542869498No Hit
GGAAGCAGTGGTATCAACGCAGAGT260.14055573575521677No Hit
AAAAAGTACTCTGCGTTGATACCAC230.12433776624499945No Hit
ATCAACGCAGAGTACATGGGAAGCA230.12433776624499945No Hit
GAGTACTTTTTTTTTTTTTTTTTTT220.11893177640826035No Hit
GCAGAGTACTTTTTTTTTTTTTTTT220.11893177640826035No Hit
GTACTTTTTTTTTTTTTTTTTTTTT190.10271380689804302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTG1451.0084477E-810.5158327
GTACTCT1552.851084E-89.8373916
ATGTACT1501.9354593E-79.5299734
CCATGTA1553.119967E-79.2225542
TGTACTC1553.119967E-79.2225545
CTGCGTT1658.1796315E-89.19109711
TCTGCGT1658.1796315E-89.19109710
CCCATGT1604.750982E-78.958761
GATACCA1701.2957389E-78.92077118
TTGATAC1701.2957389E-78.92077116
ATACCAC1701.2957389E-78.92077119
ACTCTGC1751.9385334E-78.6894428
CTCTGCG1752.0226071E-78.6658939
TGCGTTG1752.0226071E-78.66589312
TGATACC1752.0226071E-78.66589317
CGTTGAT1752.0226071E-78.66589314
CATGTAC1657.720064E-78.6636113
AGTGGTA1355.9004546E-58.42517314
GAAGCAG1150.00100942728.2420179
GTTGATA1854.7309004E-78.19746615
Produced by FastQC (version 0.11.5)