##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926991_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18498
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.40485457887339	31.0	27.0	31.0	2.0	34.0
2	26.53124662125635	31.0	27.0	31.0	2.0	34.0
3	26.41258514433993	31.0	26.0	33.0	2.0	34.0
4	29.654881608822574	35.0	30.0	37.0	2.0	37.0
5	29.736133636068764	35.0	30.0	37.0	2.0	37.0
6	29.094010163260894	35.0	29.0	37.0	2.0	37.0
7	29.03567953292248	35.0	28.0	37.0	2.0	37.0
8	28.823116012541895	35.0	28.0	37.0	2.0	37.0
9	30.562871661801275	37.0	30.0	39.0	2.0	39.0
10	30.764298843118176	37.0	31.0	39.0	2.0	39.0
11	30.84771326629906	37.0	31.0	39.0	2.0	39.0
12	30.81662882473781	37.0	31.0	39.0	2.0	39.0
13	30.84619958914477	37.0	31.0	39.0	2.0	39.0
14	31.777759757811655	38.0	31.0	40.0	2.0	41.0
15	31.25981187155368	38.0	30.0	40.0	2.0	41.0
16	31.211482322413232	38.0	30.0	40.0	2.0	41.0
17	31.551573143042493	38.0	31.0	40.0	2.0	41.0
18	31.569683208995567	38.0	31.0	40.0	2.0	41.0
19	30.934587522975455	38.0	30.0	40.0	2.0	41.0
20	31.158287382419722	38.0	30.0	40.0	2.0	41.0
21	31.13525786571521	38.0	30.0	40.0	2.0	41.0
22	31.206022272678126	38.0	30.0	40.0	2.0	41.0
23	31.204832954914046	38.0	30.0	40.0	2.0	41.0
24	29.986430965509786	38.0	26.0	40.0	2.0	41.0
25	30.54849172883555	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.674040946090912
1101	2	-1.444954226817977
1101	3	-4.589032195091086
1101	4	-2.1348742826136196
1101	5	-2.290354153009151
1101	6	-5.255776821883867
1101	7	-3.2768389564554212
1101	8	-1.6036847727007775
1101	9	-2.4642595695833904
1101	10	-6.258645393341773
1101	11	-3.887738602004049
1101	12	-2.3607183495900266
1101	13	-2.5815072550953744
1101	14	-2.0748206887412444
1101	15	-2.52289026394223
1101	16	-1.7121093993403917
1101	17	-1.6540100470131414
1101	18	-1.9842015045806178
1101	19	-3.233055051714995
1101	20	-1.8469117464442135
1101	21	-2.08866521863807
1101	22	-0.4752419549068492
1101	23	-0.9618408342259102
1101	24	-1.0719328628197111
1101	25	-0.5273367828016227
1102	1	0.47004772385982463
1102	2	0.03780439387167789
1102	3	0.3481599231355119
1102	4	0.6422193134454943
1102	5	1.2502862410795181
1102	6	-2.5242497282878062
1102	7	-1.2694497938938447
1102	8	0.9381871484814894
1102	9	-0.7130280424897961
1102	10	0.10219204508187474
1102	11	0.17014317139496526
1102	12	0.06785307898140047
1102	13	1.0207094936731025
1102	14	-0.8272837429284365
1102	15	-0.5980134166515896
1102	16	-0.8808286111630501
1102	17	-1.8301184213973798
1102	18	-3.113511849408205
1102	19	-2.885764411320906
1102	20	-0.9355816971831317
1102	21	-1.5812760560764936
1102	22	-0.9185917086014292
1102	23	-1.2167669426002945
1102	24	-4.697548626366508
1102	25	-3.6012284084173842
1103	1	-4.902876924926893
1103	2	-5.296806078669828
1103	3	-4.743794099852991
1103	4	-4.636197033936373
1103	5	-4.6858567985117965
1103	6	-4.004454070561113
1103	7	-3.7292199088363738
1103	8	-3.074584243600249
1103	9	-4.567434172757995
1103	10	-4.7573226420190196
1103	11	-5.023981988247435
1103	12	-4.746961735833413
1103	13	-4.8209252445133615
1103	14	-4.896249260169817
1103	15	-4.960720951772917
1103	16	-5.345707282938275
1103	17	-5.131523274526369
1103	18	-5.511979282358396
1103	19	-5.976441295101235
1103	20	-5.455377354909825
1103	21	-5.226231356204206
1103	22	-5.628681108346004
1103	23	-5.28062390300898
1103	24	-3.3999751908620404
1103	25	-4.003527258992097
1104	1	2.467493445443477
1104	2	1.1106013287375802
1104	3	1.8858355297766387
1104	4	2.4749140771747413
1104	5	2.2400691274141273
1104	6	-0.004454070561113355
1104	7	1.604113424496962
1104	8	2.2587490897330866
1104	9	2.9881213827975586
1104	10	2.538973654277278
1104	11	2.9019439376784923
1104	12	3.2160012271295457
1104	13	3.7716673480792267
1104	14	2.65930629538574
1104	15	2.6318716408196785
1104	16	1.6172556800246873
1104	17	1.9425507995477034
1104	18	1.9324651620860465
1104	19	1.0605957419357992
1104	20	2.5075856080531373
1104	21	2.625620495647645
1104	22	2.667615187950293
1104	23	2.0897464673613904
1104	24	5.2666914758046275
1104	25	3.7372134817486398
1105	1	-1.6890179046162608
1105	2	-0.832051001011525
1105	3	-1.216912379422883
1105	4	-2.5669019323832067
1105	5	-3.160169821211916
1105	6	-1.7189104624368596
1105	7	-2.159327435718094
1105	8	-2.728108735834418
1105	9	-2.8720936709658815
1105	10	-2.729843550023798
1105	11	-2.2736833024648817
1105	12	-2.1256953081153753
1105	13	-1.6656086375838512
1105	14	-2.1220557117827212
1105	15	-1.9272681441265611
1105	16	-1.73399880026205
1105	17	-2.148249678349547
1105	18	-3.379362794903198
1105	19	-3.25840067383481
1105	20	-2.1280177372276263
1105	21	-2.664702084997515
1105	22	-3.9130299733400307
1105	23	-4.032117331921761
1105	24	-7.698660973418789
1105	25	-6.025032635336185
1106	1	0.36167333962337267
1106	2	-0.4806685125322616
1106	3	0.1252535191946258
1106	4	0.2222685745292381
1106	5	0.3167887041337032
1106	6	0.5656517495447062
1106	7	-2.383981813598279
1106	8	1.0748866558706531
1106	9	1.3214547161308943
1106	10	2.0270688923725118
1106	11	0.2908328265673781
1106	12	1.1035673646956852
1106	13	1.0613498877617715
1106	14	0.8894650255444674
1106	15	1.155681164629197
1106	16	1.0378906006596118
1106	17	0.2031328101297163
1106	18	-0.12705864743776019
1106	19	-0.5544836231435646
1106	20	0.8673739678414982
1106	21	0.9827633527904993
1106	22	0.7747580450931473
1106	23	0.6453020229169475
1106	24	-0.7505042913911382
1106	25	-0.6344796399444803
1107	1	2.1045304824805164
1107	2	0.38837910651535523
1107	3	2.356205900147007
1107	4	1.4675066697673316
1107	5	1.3215506088956097
1107	6	1.0918422257351814
1107	7	0.4374467578302941
1107	8	-0.7560657250817293
1107	9	1.2214547161308928
1107	10	1.9056403209439452
1107	11	0.5908328265673788
1107	12	1.6345197456480705
1107	13	1.749445125857008
1107	14	0.7370840731635155
1107	15	-1.4014616925136565
1107	16	0.4617001244691359
1107	17	-0.4944862374893333
1107	18	0.465798495419385
1107	19	0.9383735197135792
1107	20	0.7149930154605464
1107	21	0.9589538289809809
1107	22	0.9676151879502903
1107	23	1.111968689583616
1107	24	-2.648123339010187
1107	25	-0.9701939256587657
1108	1	2.1473876253376574
1108	2	0.9836172017534501
1108	3	1.9466820906231987
1108	4	1.6151257173863804
1108	5	1.6025029898479914
1108	6	-2.827205393312436
1108	7	-0.20541038502684827
1108	8	0.7534580844420802
1108	9	1.0357404304166096
1108	10	2.6699260352296577
1108	11	0.5408328265673781
1108	12	0.31785307898140047
1108	13	0.9184927449046256
1108	14	1.5323221684016133
1108	15	0.15568116462920045
1108	16	0.4664620292310353
1108	17	1.381704238701147
1108	18	1.2657984954193822
1108	19	2.2312306625707237
1108	20	-2.0969117464442135
1108	21	-1.1600937900666395
1108	22	-0.8323848120497068
1108	23	0.07387345148837454
1108	24	0.4280671371802889
1108	25	0.15123464576980794
1109	1	2.0971230750731067
1109	2	1.555045773182023
1109	3	-1.6326829887418803
1109	4	1.2897288919895544
1109	5	1.351180238525238
1109	6	2.662212596105551
1109	7	3.7152245356080726
1109	8	2.592082423066419
1109	9	2.4696028642790395
1109	10	2.8352699505735686
1109	11	3.0130550487895995
1109	12	1.7345197456480648
1109	13	3.1420377184496004
1109	14	3.177824813904259
1109	15	0.2985383074863428
1109	16	2.024663087432092
1109	17	1.9425507995477034
1109	18	2.710242939863825
1109	19	1.1346698160098754
1109	20	2.9149930154605492
1109	21	2.3293241993513476
1109	22	3.112059632394736
1109	23	2.978635356250283
1109	24	3.007432216545368
1109	25	4.255732000267159
1110	1	0.43556496523913424
1110	2	-0.10012664061108012
1110	3	0.2447116472734443
1110	4	-0.9095048244855413
1110	5	0.49166555142434376
1110	6	-0.7656290386326354
1110	7	0.2133088267958101
1110	8	0.7312905967573506
1110	9	0.6317995437171007
1110	10	0.5849506657715295
1110	11	-0.5195120010188283
1110	12	0.7919910100158845
1110	13	-0.46204912701655587
1110	14	-0.7238354670663689
1110	15	-0.5290478994102088
1110	16	0.8433093198714339
1110	17	0.06643330274055259
1110	18	-0.25144288389096303
1110	19	-3.0926609630450415
1110	20	0.34028037178238435
1110	21	-0.8916208836627
1110	22	-0.47031584653246483
1110	23	-0.3891807357037429
1110	24	-0.9389279367113375
1110	25	-1.1184697877277294
1111	1	0.366599447997757
1111	2	1.0033216352509875
1111	3	0.8998840610665475
1111	4	1.9525641410317007
1111	5	0.8364931376312406
1111	6	1.8205778579190905
1111	7	1.21330882679581
1111	8	1.9726699071021798
1111	9	1.0111098885446879
1111	10	0.6539161830129068
1111	11	0.032212136912207257
1111	12	1.205784113464162
1111	13	0.2965715626386185
1111	14	0.862371429485357
1111	15	0.953710721279446
1111	16	0.8433093198714339
1111	17	1.2043643372233106
1111	18	1.438212288522827
1111	19	1.6314769679894425
1111	20	1.0299355441961815
1111	21	0.6601032542683356
1111	22	1.460718636226158
1111	23	1.8177158160203923
1111	24	1.923141028805908
1111	25	2.2953233157205446
1112	1	2.0907373790322374
1112	2	1.0722871524923683
1112	3	1.8998840610665475
1112	4	1.1249779341351491
1112	5	2.9744241721139986
1112	6	1.544715788953571
1112	7	1.9719295164509845
1112	8	1.8692216312401086
1112	9	2.3214547161308943
1112	10	2.3090885968060135
1112	11	1.5839362748432428
1112	12	1.6195772169124325
1112	13	1.2620888040179281
1112	14	2.0692679812094923
1112	15	2.126124514382898
1112	16	2.0157231129748787
1112	17	2.962985026878485
1112	18	2.438212288522827
1112	19	3.0107873128170226
1112	20	2.823038992472039
1112	21	1.694586012889026
1112	22	2.184856567260635
1112	23	0.9901296091238443
1112	24	1.923141028805908
1112	25	0.7091164191688222
1113	1	0.07119714914718145
1113	2	0.28837910651535736
1113	3	-6.977127433186325
1113	4	-2.599159996899335
1113	5	-0.41178272443772457
1113	6	1.8918422257351821
1113	7	1.5041134244969605
1113	8	2.077267608251603
1113	9	0.25478804946422784
1113	10	1.3723069876106067
1113	11	1.0908328265673752
1113	12	0.9345197456480676
1113	13	-1.0505548741429926
1113	14	-0.662915926836483
1113	15	0.23187164081967637
1113	16	1.328366791135796
1113	17	0.47218042917733527
1113	18	2.1324651620860493
1113	19	2.638373519713575
1113	20	2.7816596821272164
1113	21	2.392287162314311
1113	22	2.7009485212836246
1113	23	1.3453020229169503
1113	24	3.185209994323145
1113	25	2.263139407674565
1114	1	-0.18512468993327857
1114	2	-1.444954226817977
1114	3	-0.9966676630713813
1114	4	-1.11640137620968
1114	5	0.11235520659676013
1114	6	0.544715788953571
1114	7	1.2822743440371909
1114	8	1.2140492174470054
1114	9	-0.5061314907656573
1114	10	-1.6564286445732996
1114	11	-0.6229602768808995
1114	12	-1.8976641623979091
1114	13	-1.255152575292417
1114	14	-0.7928009843077497
1114	15	-3.425599623548141
1114	16	-0.7084148180596017
1114	17	-0.24391152484565382
1114	18	0.438212288522827
1114	19	1.4590631748859906
1114	20	0.5816596821272135
1114	21	0.9014825646131612
1114	22	0.7020979465709836
1114	23	0.2659916780893603
1114	24	1.1645203391507337
1114	25	1.7435991777895126
1115	1	-2.0837048116371335
1115	2	-0.3273071679944479
1115	3	0.3836568805391636
1115	4	0.5184870619241941
1115	5	1.0058643343858051
1115	6	1.4290971276959645
1115	7	1.2609761695950006
1115	8	1.3458950592319958
1115	9	1.6155723631897203
1115	10	0.6468167915321779
1115	11	0.20259753244972956
1115	12	1.1560883730990454
1115	13	0.3533666944844569
1115	14	1.0449272104184146
1115	15	0.7103030133686978
1115	16	0.42444522250834993
1115	17	1.621200037020472
1115	18	1.8540337895370271
1115	19	-4.39692059793348
1115	20	-0.830105023755138
1115	21	1.2726793191770582
1115	22	0.7264387173620612
1115	23	0.468831434681654
1115	24	-0.891260593912147
1115	25	-0.14078216095288099
1116	1	1.739824600127573
1116	2	1.702104596711436
1116	3	1.4130686452450476
1116	4	0.7537811795712521
1116	5	1.5646878637975696
1116	6	2.6938030100489065
1116	7	1.1139173460655876
1116	8	1.3164832945261153
1116	9	1.1743958926014813
1116	10	-0.02965379670311563
1116	11	1.23200929715561
1116	12	1.1266766083931685
1116	13	1.529837282719754
1116	14	1.3684566221831247
1116	15	0.2985383074863428
1116	16	0.5126805166259949
1116	17	1.150611801726356
1116	18	1.0305043777723242
1116	19	1.3383735197135778
1116	20	1.6110714468330976
1116	21	0.4785616721182322
1116	22	0.3146740114797062
1116	23	-0.11940385943599452
1116	24	0.579327641381969
1116	25	0.6239237214000575
1201	1	-0.10261237466234263
1201	2	1.126474344610596
1201	3	1.6966820906231987
1201	4	0.7222685745292381
1201	5	0.6382172755622761
1201	6	2.172794606687564
1201	7	1.8660181864017211
1201	8	2.25345808444208
1201	9	1.857169001845179
1201	10	1.4199260352296577
1201	11	2.7194042551388087
1201	12	1.6392816504099699
1201	13	2.382778459190341
1201	14	2.1037507398301827
1201	15	2.905681164629197
1201	16	2.537890600659612
1201	17	2.2745613815582857
1201	18	2.051512781133667
1201	19	2.2312306625707237
1201	20	2.4388025392700676
1201	21	1.4470490670762146
1201	22	1.4176151879502932
1201	23	1.7524448800598016
1201	24	-1.2505042913911382
1201	25	0.47266321719837734
1202	1	1.004530482480515
1202	2	0.8627380808743297
1202	3	-0.3719992280581188
1202	4	0.5162246184852819
1202	5	1.8112941986391995
1202	6	3.0354319693249288
1202	7	2.168215988599524
1202	8	2.764447095431091
1202	9	3.0522239469001278
1202	10	1.3979480132516358
1202	11	1.0600635957981446
1202	12	1.337083848212167
1202	13	0.7289323053441876
1202	14	0.02682766290710603
1202	15	1.7985383074863428
1202	16	0.9719565347255461
1202	17	2.238847095844001
1202	18	1.7657984954193822
1202	19	2.492219673559731
1202	20	2.773967374434907
1202	21	2.625620495647645
1202	22	2.9753074956425998
1202	23	3.6837635613784876
1202	24	3.7467484558616064
1202	25	4.945190689725848
1203	1	0.8506843286343617
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2211	7	0.4017324721160094
2211	8	1.2534580844420802
2211	9	-0.10711671244053278
2211	10	3.134211749515373
2211	11	1.3979756837102322
2211	12	2.5321387932671158
2211	13	2.239921316333195
2211	14	2.1394650255444674
2211	15	2.405681164629197
2211	16	3.037890600659612
2211	17	2.3102756672725704
2211	18	2.6943699239908128
2211	19	3.1240878054278625
2211	20	1.9745168249843594
2211	21	1.9827633527904993
2211	22	-4.725241954906849
2211	23	-0.8546979770830525
2211	24	1.7852099943231465
2211	25	0.04409178862695029
2212	1	-0.19917322122318737
2212	2	0.18467540281165284
2212	3	-1.743794099852991
2212	4	-1.2658266635660027
2212	5	1.4993283866733869
2212	6	2.18073111462407
2212	7	1.3448541652377024
2212	8	1.4068972378812354
2212	9	1.0992324939086728
2212	10	1.6130477283513471
2212	11	1.0130550487895995
2212	12	-0.19140618027785905
2212	13	0.03092660733848973
2212	14	0.9185655546449993
2212	15	0.3355753445233809
2212	16	-0.567929505160496
2212	17	1.275884132881039
2212	18	0.4139466435675274
2212	19	1.1346698160098754
2212	20	-1.5294514289838972
2212	21	-0.004009133981984547
2212	22	0.18613370646881222
2212	23	-0.7621053844904608
2212	24	-0.21479000567685347
2212	25	-0.15167540714024597
2213	1	-5.151719517519485
2213	2	-5.226204226817977
2213	3	-4.347960766519659
2213	4	-9.849159996899335
2213	5	-6.205532724437724
2213	6	-6.1664911075981514
2213	7	-3.593803242169706
2213	8	-7.483149058415062
2213	9	-4.616045283869106
2213	10	-4.825609679056058
2213	11	-4.115417173432622
2213	12	-3.3383969210185995
2213	13	-2.554721540809659
2213	14	-3.7399992601698173
2213	15	-4.545211692513657
2213	16	-3.073716542197534
2213	17	-3.386152904155999
2213	18	-3.077951504580618
2213	19	-3.7241264802864222
2213	20	-6.2933403178727865
2213	21	-5.343129504352355
2213	22	-6.144884812049707
2213	23	-5.9796979770830525
2213	24	-5.4335400056768535
2213	25	-6.076443925658765
2214	1	3.6045304824805164
2214	2	3.8883791065153552
2214	3	3.1895392334803425
2214	4	3.034173336433998
2214	5	3.6215506088956104
2214	6	2.4585088924018486
2214	7	4.5374467578302955
2214	8	1.8772676082516035
2214	9	2.954788049464227
2214	10	3.0056403209439395
2214	11	2.9574994932340424
2214	12	1.8345197456480662
2214	13	3.1494451258570066
2214	14	3.837084073163517
2214	15	2.531871640819677
2214	16	3.9950334578024673
2214	17	4.172180429177331
2214	18	3.465798495419385
2214	19	4.838373519713578
2214	20	2.6483263487938835
2214	21	3.258953828980978
2214	22	4.500948521283629
2214	23	2.2786353562502804
2214	24	-1.0481233390101856
2214	25	-0.63686059232543
2215	1	3.595439573389605
2215	2	3.676257894394144
2215	3	3.9834786274197356
2215	4	4.400840003100665
2215	5	4.539732427077425
2215	6	4.049417983310942
2215	7	3.9980528184363564
2215	8	2.322722153706149
2215	9	3.2305456252218008
2215	10	3.751094866398489
2215	11	4.245378281112831
2215	12	4.0678530789814005
2215	13	3.5494451258570052
2215	14	4.437084073163518
2215	15	4.207629216577249
2215	16	4.667760730529739
2215	17	3.9055137625106653
2215	18	3.0839803136012023
2215	19	4.686858368198429
2215	20	2.1877202881878226
2215	21	3.6862265562537075
2215	22	4.879736400071504
2215	23	4.4331808107957364
2215	24	4.482179691292842
2215	25	3.6025333470685084
2216	1	-0.26966306590658107
2216	2	-0.9288251945599129
2216	3	-0.3459446374873991
2216	4	-0.018514835609011016
2216	5	0.0011205013687280996
2216	6	-0.009233043082023329
2216	7	-8.22384356475035
2216	8	-10.889399058415062
2216	9	-5.130158187094914
2216	10	-2.1814564532496057
2216	11	-0.7898123347229458
2216	12	-0.9321469210185995
2216	13	-1.343027992422563
2216	14	-1.3478621633956251
2216	15	-1.024042337674949
2216	16	-1.346902026068502
2216	17	-0.9547012912527748
2216	18	-0.3471047303870698
2216	19	-0.06485228673803434
2216	20	0.2268209724497936
2216	21	-0.7292182140297747
2216	22	-0.23561061850131892
2216	23	0.4194955713040436
2216	24	-1.0857577476123375
2216	25	0.6523867195025268
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2027.0
3	1.0
4	17.0
5	107.0
6	181.0
7	134.0
8	74.0
9	63.0
10	47.0
11	54.0
12	49.0
13	43.0
14	49.0
15	51.0
16	40.0
17	64.0
18	51.0
19	67.0
20	108.0
21	99.0
22	139.0
23	143.0
24	168.0
25	204.0
26	243.0
27	244.0
28	217.0
29	317.0
30	307.0
31	406.0
32	540.0
33	725.0
34	902.0
35	1370.0
36	1965.0
37	3199.0
38	4083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.927615538819964	18.540391179498293	11.144823102345994	22.38717017933575
2	15.570353527150669	23.344702506632018	34.199556060852146	26.885387905365164
3	22.331095712429626	30.40818536162841	25.6388046773495	21.621914248592464
4	13.614972683507329	21.355547141234382	28.776978417266186	36.2525017579921
5	13.008921330089212	42.35198702351987	29.667477696674776	14.97161394971614
6	32.02487158691538	32.73317112733171	17.26412543930792	17.977831846444985
7	24.849536409477853	37.19026188797918	18.635796779265846	19.324404923277125
8	28.097381120208208	33.530336713116085	19.35151548012796	19.02076668654774
9	27.618275209516085	18.740200054068666	17.44255204109219	36.198972695323064
10	18.77365632096896	32.68627663025846	25.857034713961287	22.68303233481129
11	29.927007299270077	28.67802108678021	16.804541768045418	24.5904298459043
12	23.665855636658556	33.538794268721276	24.20654230873209	18.58880778588808
13	30.078399567450663	24.920248715869153	20.524466071911327	24.476885644768856
14	22.389835090565015	29.00783995674507	22.249256555825898	26.353068396864014
15	31.78156258448229	26.677480400108138	19.626926196269263	21.914030819140308
16	19.9080832657475	31.094890510948904	26.142200594755337	22.854825628548255
17	24.20654230873209	32.11138145444715	22.622330359556635	21.059745877264124
18	23.746958637469586	27.64530954311976	25.85563665855637	22.752095160854285
19	23.61719383617194	31.0029737766964	26.812652068126518	18.567180319005136
20	24.698567180319007	27.531765341984322	25.52581778859151	22.243849689105165
21	23.60638010273047	29.240335225736686	26.407137064071375	20.746147607461477
22	24.666125979994593	28.688834820221683	21.21654501216545	25.428494187618277
23	21.91943768586104	29.278183292781833	25.439307921059743	23.36307110029738
24	23.391370174110524	31.95631015464475	22.49918892613821	22.153130745106523
25	23.206271965396052	27.039740470397405	21.995133819951338	27.7588537442552
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	227.0
2	200.0
3	173.0
4	173.0
5	173.0
6	167.5
7	162.0
8	162.0
9	162.0
10	158.0
11	154.0
12	154.0
13	154.0
14	155.0
15	156.0
16	156.0
17	156.0
18	207.0
19	258.0
20	258.0
21	258.0
22	321.5
23	385.0
24	385.0
25	385.0
26	460.0
27	535.0
28	535.0
29	535.0
30	631.0
31	727.0
32	727.0
33	727.0
34	790.0
35	853.0
36	853.0
37	853.0
38	920.5
39	988.0
40	988.0
41	988.0
42	1203.5
43	1419.0
44	1419.0
45	1419.0
46	2594.5
47	3770.0
48	3770.0
49	3770.0
50	3429.5
51	3089.0
52	3089.0
53	3089.0
54	2447.5
55	1806.0
56	1806.0
57	1806.0
58	1520.0
59	1234.0
60	1234.0
61	1234.0
62	1141.0
63	1048.0
64	1048.0
65	1048.0
66	896.0
67	744.0
68	744.0
69	744.0
70	585.0
71	426.0
72	426.0
73	426.0
74	324.5
75	223.0
76	223.0
77	223.0
78	157.5
79	92.0
80	92.0
81	92.0
82	57.0
83	22.0
84	22.0
85	22.0
86	14.0
87	6.0
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22164558330630338
2	0.1459617255919559
3	0.14055573575521677
4	0.05946588820413017
5	0.01621796951021732
6	0.01621796951021732
7	0.2973294410206509
8	0.2973294410206509
9	0.01621796951021732
10	0.021623959346956427
11	0.01621796951021732
12	0.01621796951021732
13	0.01621796951021732
14	0.01621796951021732
15	0.01621796951021732
16	0.01621796951021732
17	0.01621796951021732
18	0.01621796951021732
19	0.01621796951021732
20	0.01621796951021732
21	0.01621796951021732
22	0.01621796951021732
23	0.01621796951021732
24	0.021623959346956427
25	0.01621796951021732
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	18498.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.7155368147908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.24207379435136	64.0717915450319
2	4.248288883644088	5.838469023678235
3	0.8181889701833058	1.6866688290626013
4	0.4484304932735426	1.2325656827765163
5	0.19668004090944852	0.6757487295923884
6	0.1809456376366926	0.7460265974699967
7	0.11014082290929117	0.5297870040004325
8	0.07080481472740147	0.3892312682452157
9	0.09440641963653527	0.5838469023678236
>10	0.3776256785461411	5.103254405881717
>50	0.11014082290929117	5.0059465888204135
>100	0.09440641963653527	11.363390636825603
>500	0.00786720163637794	2.7732727862471616
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	513	2.7732727862471616	No Hit
CCCATGTACTCTGCGTTGATACCAC	432	2.335387609471294	No Hit
GAGTACATGGGAAGCAGTGGTATCA	251	1.356903449021516	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	186	1.0055141096334739	No Hit
GCGTTGATACCACTGCTTCCCATGT	168	0.9082062925721699	No Hit
CATGTACTCTGCGTTGATACCACTG	164	0.8865823332252135	No Hit
GGTATCAACGCAGAGTACATGGGAA	153	0.8271164450210833	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	138	0.7460265974699967	No Hit
TATCAACGCAGAGTACATGGGAAGC	137	0.7406206076332577	No Hit
GTATCAACGCAGAGTACTTTTTTTT	133	0.7189966482863013	No Hit
ACTCTGCGTTGATACCACTGCTTCC	123	0.6649367499189102	No Hit
GTATCAACGCAGAGTACATGGGAAG	113	0.610876851551519	No Hit
GGTATCAACGCAGAGTACTTTTTTT	104	0.5622229430208671	No Hit
GTGGTATCAACGCAGAGTACATGGG	91	0.49194507514325875	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	88	0.4757271056330414	No Hit
GCTTCCCATGTACTCTGCGTTGATA	78	0.4216672072656503	No Hit
TATCAACGCAGAGTACTTTTTTTTT	78	0.4216672072656503	No Hit
GCAGTGGTATCAACGCAGAGTACAT	71	0.3838252784084766	No Hit
GTACTCTGCGTTGATACCACTGCTT	71	0.3838252784084766	No Hit
CAGTGGTATCAACGCAGAGTACATG	62	0.33517136987782464	No Hit
GTTGATACCACTGCTTCCCATGTAC	58	0.3135474105308682	No Hit
CATGGGAAGCAGTGGTATCAACGCA	57	0.3081414206941291	No Hit
ATACCACTGCTTCCCATGTACTCTG	57	0.3081414206941291	No Hit
GATACCACTGCTTCCCATGTACTCT	55	0.2973294410206509	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	55	0.2973294410206509	No Hit
ACATGGGAAGCAGTGGTATCAACGC	54	0.2919234511839118	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	51	0.27570548167369446	No Hit
TACCACTGCTTCCCATGTACTCTGC	50	0.27029949183695534	No Hit
CTTCCCATGTACTCTGCGTTGATAC	49	0.2648935020002163	No Hit
CCACTGCTTCCCATGTACTCTGCGT	44	0.2378635528165207	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	44	0.2378635528165207	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	42	0.22705157314304247	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	40	0.21623959346956426	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	37	0.20002162395934697	No Hit
GTACTGGTTCACTATCGGTCAGTCA	31	0.16758568493891232	No Hit
TTCCCATGTACTCTGCGTTGATACC	31	0.16758568493891232	No Hit
CCATGTACTCTGCGTTGATACCACT	30	0.1621796951021732	No Hit
TGATACCACTGCTTCCCATGTACTC	28	0.15136771542869498	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	26	0.14055573575521677	No Hit
AAAAAGTACTCTGCGTTGATACCAC	23	0.12433776624499945	No Hit
ATCAACGCAGAGTACATGGGAAGCA	23	0.12433776624499945	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	22	0.11893177640826035	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	22	0.11893177640826035	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	19	0.10271380689804302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCTG	145	1.0084477E-8	10.515832	7
GTACTCT	155	2.851084E-8	9.837391	6
ATGTACT	150	1.9354593E-7	9.529973	4
CCATGTA	155	3.119967E-7	9.222554	2
TGTACTC	155	3.119967E-7	9.222554	5
CTGCGTT	165	8.1796315E-8	9.191097	11
TCTGCGT	165	8.1796315E-8	9.191097	10
CCCATGT	160	4.750982E-7	8.95876	1
GATACCA	170	1.2957389E-7	8.920771	18
TTGATAC	170	1.2957389E-7	8.920771	16
ATACCAC	170	1.2957389E-7	8.920771	19
ACTCTGC	175	1.9385334E-7	8.689442	8
CTCTGCG	175	2.0226071E-7	8.665893	9
TGCGTTG	175	2.0226071E-7	8.665893	12
TGATACC	175	2.0226071E-7	8.665893	17
CGTTGAT	175	2.0226071E-7	8.665893	14
CATGTAC	165	7.720064E-7	8.663611	3
AGTGGTA	135	5.9004546E-5	8.425173	14
GAAGCAG	115	0.0010094272	8.242017	9
GTTGATA	185	4.7309004E-7	8.197466	15
>>END_MODULE