FastQCFastQC Report
Thu 26 May 2016
SRR1926991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18498
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC5743.1030381662882474No Hit
CCCATGTACTCTGCGTTGATACCAC5442.940858471186074No Hit
GTATCAACGCAGAGTACTTTTTTTT3461.870472483511731No Hit
GAGTACATGGGAAGCAGTGGTATCA2491.3460914693480377No Hit
GGTATCAACGCAGAGTACTTTTTTT2401.2974375608173856No Hit
ACGCAGAGTACATGGGAAGCAGTGG2231.2055357335928207No Hit
CATGTACTCTGCGTTGATACCACTG2171.1730997945723862No Hit
TATCAACGCAGAGTACTTTTTTTTT1740.9406422315926046No Hit
GCGTTGATACCACTGCTTCCCATGT1690.913612282408909No Hit
GGTATCAACGCAGAGTACATGGGAA1610.8703643637149961No Hit
GTATCAACGCAGAGTACATGGGAAG1540.8325224348578224No Hit
ACTCTGCGTTGATACCACTGCTTCC1440.7784625364904314No Hit
TATCAACGCAGAGTACATGGGAAGC1380.7460265974699967No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1290.6973726889393448No Hit
GTGGTATCAACGCAGAGTACATGGG1080.5838469023678236No Hit
GATACCACTGCTTCCCATGTACTCT820.44329116661260676No Hit
CAGTGGTATCAACGCAGAGTACATG780.4216672072656503No Hit
GCTTCCCATGTACTCTGCGTTGATA780.4216672072656503No Hit
ATACCACTGCTTCCCATGTACTCTG730.39463725808195477No Hit
CATGGGAAGCAGTGGTATCAACGCA710.3838252784084766No Hit
GCAGTGGTATCAACGCAGAGTACAT690.37301329873499833No Hit
GTACTTTTTTTTTTTTTTTTTTTTT650.35138933938804195No Hit
CCACTGCTTCCCATGTACTCTGCGT630.3405773597145637No Hit
GTACTCTGCGTTGATACCACTGCTT630.3405773597145637No Hit
ACATGGGAAGCAGTGGTATCAACGC620.33517136987782464No Hit
TACCACTGCTTCCCATGTACTCTGC580.3135474105308682No Hit
GGGAAGCAGTGGTATCAACGCAGAG570.3081414206941291No Hit
CTGCTTCCCATGTACTCTGCGTTGA560.30273543085738996No Hit
AAGCAGTGGTATCAACGCAGAGTAC550.2973294410206509No Hit
ATGGGAAGCAGTGGTATCAACGCAG520.28111147151043353No Hit
GCAGAGTACATGGGAAGCAGTGGTA510.27570548167369446No Hit
GAGTACTTTTTTTTTTTTTTTTTTT490.2648935020002163No Hit
CTTCCCATGTACTCTGCGTTGATAC480.2594875121634771No Hit
CCATGTACTCTGCGTTGATACCACT460.2486755324899989No Hit
GCAGAGTACTTTTTTTTTTTTTTTT460.2486755324899989No Hit
GTACTGGTTCACTATCGGTCAGTCA450.2432695426532598No Hit
ATCAACGCAGAGTACTTTTTTTTTT430.2324575629797816No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA420.22705157314304247No Hit
ATCAACGCAGAGTACATGGGAAGCA420.22705157314304247No Hit
GTTGATACCACTGCTTCCCATGTAC410.22164558330630338No Hit
TTCCCATGTACTCTGCGTTGATACC350.18920964428586876No Hit
GTGGTATCAACGCAGAGTACTTTTT340.18380365444912963No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA280.15136771542869498No Hit
TGATACCACTGCTTCCCATGTACTC270.1459617255919559No Hit
GGATACCACGTGTCCCGCCCTACTC230.12433776624499945No Hit
GTACATGGTAAGCAGTGGTATCAAC220.11893177640826035No Hit
GTACATGGAAAGCAGTGGTATCAAC190.10271380689804302No Hit
CTATCGGTCAGTCAGGAGTATTTAG190.10271380689804302No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA190.10271380689804302No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT1409.061734E-58.12195114
TACTCTG1451.3642006E-47.84188377
CCCATGT1355.1330804E-47.7841531
CCATGTA1501.8370937E-47.6426232
CTCTGCG1502.0222303E-47.58048779
GTTGATA1502.0222303E-47.580487715
TGCGTTG1502.0222303E-47.580487712
CTGCGTT1502.0222303E-47.580487711
TCTGCGT1502.0222303E-47.580487710
ACTCTGC1552.954678E-47.33595568
TGATACC1552.954678E-47.335955617
TTGATAC1552.954678E-47.335955616
GCGTTGA1552.954678E-47.335955613
GTACTCT1603.8721896E-47.1649596
TGTACTC1603.8721896E-47.1649595
ATGTACT1707.757069E-46.74349074
CATGTAC1707.757069E-46.74349073
AACGCAG2702.4557085E-66.36885266
GATACCA1850.00222239786.14634218
GTATCAA3052.7076876E-65.95122341
Produced by FastQC (version 0.11.5)