##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926991_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18498 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.1095794139907 31.0 30.0 33.0 25.0 34.0 2 29.205481673694454 31.0 30.0 33.0 24.0 34.0 3 29.233646880743866 31.0 30.0 34.0 23.0 34.0 4 32.758081954805924 35.0 33.0 37.0 26.0 37.0 5 32.82019677803006 35.0 33.0 37.0 26.0 37.0 6 32.72067250513569 36.0 35.0 37.0 25.0 37.0 7 32.70818466861282 36.0 35.0 37.0 25.0 37.0 8 32.672721375283814 37.0 35.0 37.0 25.0 37.0 9 34.081089847551084 39.0 35.0 39.0 23.0 39.0 10 33.96129311276895 38.0 35.0 39.0 23.0 39.0 11 33.935993080333006 39.0 35.0 39.0 23.0 39.0 12 33.92837063466321 39.0 35.0 39.0 23.0 39.0 13 33.818953400367604 39.0 35.0 39.0 21.0 39.0 14 34.811709373986375 39.0 36.0 41.0 20.0 41.0 15 34.68212779759974 39.0 35.0 41.0 18.0 41.0 16 34.55141096334739 39.0 35.0 41.0 17.0 41.0 17 34.6443939885393 39.0 35.0 41.0 19.0 41.0 18 34.594334522651096 39.0 35.0 41.0 17.0 41.0 19 34.59395610336252 39.0 35.0 41.0 18.0 41.0 20 34.59157746783436 39.0 35.0 41.0 18.0 41.0 21 34.49610768731755 39.0 35.0 41.0 16.0 41.0 22 34.40220564385339 39.0 35.0 41.0 10.0 41.0 23 34.35598443074927 39.0 35.0 41.0 8.0 41.0 24 34.26251486647205 39.0 35.0 41.0 2.0 41.0 25 34.115309763217645 39.0 35.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.1794290707980757 1101 2 -1.0586498306627767 1101 3 -1.8327129890309948 1101 4 -2.8920732159780194 1101 5 -4.95231483441459 1101 6 -3.8912317742187383 1101 7 -4.950133121673549 1101 8 -3.9938752094530017 1101 9 -5.310977034348376 1101 10 -5.556070251030519 1101 11 -5.078771066989873 1101 12 -5.9001124897306845 1101 13 -5.441202869963817 1101 14 -6.196597248131205 1101 15 -6.462102881572246 1101 16 -6.949568424557167 1101 17 -6.739619043646041 1101 18 -6.518733249743878 1101 19 -5.797169491892912 1101 20 -5.522179244213152 1101 21 -5.711784644444069 1101 22 -5.168884206579023 1101 23 -5.162657550384086 1101 24 -5.203140075142841 1101 25 -5.21845545186768 1102 1 -1.1708083811429049 1102 2 -1.8209651015987376 1102 3 -1.6061120038093186 1102 4 -0.9573441519386101 1102 5 -0.8710340462372486 1102 6 -0.744680050080806 1102 7 -1.0757488852203458 1102 8 -1.1157963917190088 1102 9 -1.086839103313892 1102 10 -0.8048387239369248 1102 11 -1.3792636778273106 1102 12 -0.6882898296321613 1102 13 -0.9596757763677601 1102 14 -0.5512770510868705 1102 15 -1.2515117485673173 1102 16 -1.1330659615029788 1102 17 -1.5265648564539234 1102 18 0.17338497685710763 1102 19 0.3210571090923082 1102 20 -0.17488860381906335 1102 21 -0.8706516395179591 1102 22 -0.8252881474657201 1102 23 -0.68605656516241 1102 24 -0.6514159372118087 1102 25 -1.6962879641829502 1103 1 -1.1516512930202971 1103 2 -1.5732000952130427 1103 3 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-0.14064007514284071 2213 25 -0.6380983090105374 2214 1 1.0372375958685893 2214 2 1.0675406455277 2214 3 0.9720489157309125 2214 4 -0.8515970255018281 2214 5 1.2048280227282646 2214 6 1.1921015591145974 2214 7 -0.3929902645306882 2214 8 -1.929589495167285 2214 9 0.02235629898495972 2214 10 -0.8370226319829044 2214 11 -2.193056781275587 2214 12 -0.390588680206875 2214 13 -1.0435838223447718 2214 14 -0.3489782005121569 2214 15 -1.5549600244293913 2214 16 -1.5043303293190746 2214 17 -1.0277142817412823 2214 18 -0.7473046783153023 2214 19 -0.6352647299881511 2214 20 -0.45075067278457936 2214 21 -1.01892750158693 2214 22 -0.27126515895997727 2214 23 -0.9412289789555146 2214 24 -0.9031400751428436 2214 25 -0.34226497567720315 2215 1 2.2220860807170766 2215 2 2.7736012515883033 2215 3 2.162958006640004 2215 4 2.9120393381345338 2215 5 2.7684643863646343 2215 6 2.601192468205504 2215 7 2.194888523348098 2215 8 3.4098044442266513 2215 9 3.6435684201970773 2215 10 3.9569167619564922 2215 11 3.3887614005425917 2215 12 4.212441622823427 2215 13 4.462476783715836 2215 14 4.305567254033299 2215 15 3.4026157331463693 2215 16 4.516881791893049 2215 17 4.651073597046597 2215 18 4.401180170169546 2215 19 4.14655345183003 2215 20 4.609855387821483 2215 21 4.832587649928222 2215 22 4.068128780433966 2215 23 4.325437687711151 2215 24 4.281708409705644 2215 25 4.833492600080369 2216 1 0.16734512275031221 2216 2 0.06001376380726953 2216 3 -0.7451553853443578 2216 4 0.7957148024551586 2216 5 0.01773124853471586 2216 6 1.3587682257812617 2216 7 -0.818796716143595 2216 8 -0.2070088500059981 2216 9 -0.4883963891870877 2216 10 0.3909343572644133 2216 11 0.17468515420828368 2216 12 -0.015319863002574152 2216 13 -0.3156268330974541 2216 14 -0.6113437919100093 2216 15 1.1289109433125475 2216 16 -0.10970667340509266 2216 17 -0.20620890539719738 2216 18 0.359146934587919 2216 19 0.8894664528075467 2216 20 0.7105396497960683 2216 21 0.7778466919614573 2216 22 -0.17234042777717917 2216 23 0.7878032791090064 2216 24 0.4420212151797429 2216 25 0.8598855619572063 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 737.0 3 1.0 4 11.0 5 87.0 6 139.0 7 97.0 8 67.0 9 53.0 10 50.0 11 45.0 12 59.0 13 32.0 14 35.0 15 42.0 16 27.0 17 39.0 18 50.0 19 55.0 20 80.0 21 96.0 22 99.0 23 92.0 24 122.0 25 129.0 26 156.0 27 173.0 28 210.0 29 237.0 30 304.0 31 403.0 32 505.0 33 645.0 34 871.0 35 1284.0 36 1780.0 37 3292.0 38 6394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.3792394655704 19.21350137934765 9.628387515551468 23.77887163953048 2 15.707174548209068 21.967319554160806 34.103452007358506 28.222053890271614 3 22.02454982966528 28.983939869139675 26.11258313956632 22.878927161628724 4 13.823116012541897 19.62374310736296 29.700508163044653 36.85263271705049 5 12.94193966915342 38.66363931235809 31.84128013839334 16.553140880095146 6 32.56860830892722 31.98286148172253 17.236142748671224 18.212387460679032 7 25.225712277666652 34.68670595231659 19.630210304373684 20.457371465643075 8 29.894566098945663 31.20302784536361 19.49175452825088 19.41065152743985 9 28.47256015139227 14.58772641254393 19.302514193025143 37.63719924303866 10 19.715644934587523 29.862687858146824 26.80289761055249 23.618769596713157 11 30.81414206941291 25.932533246837497 17.245107579197754 26.008217104551846 12 25.554113958265756 30.419504811330956 25.327062385122716 18.699318845280573 13 31.435830900637907 20.623851227159694 23.218726348794462 24.721591523407934 14 24.013406854795114 26.040653043572277 23.79176127148881 26.1541788301438 15 32.808952319169634 22.2834901070386 22.41323386312034 22.494323710671424 16 21.386095794139905 27.381338523083578 28.743647961941832 22.488917720834685 17 24.27289436695859 28.46794248026814 25.23516055789815 22.02400259487512 18 24.59725375716294 23.289004216672073 29.381554762677048 22.732187263487944 19 23.2511622878149 27.47864634014488 31.041193642555953 18.22899772948427 20 24.840523299816198 22.81327711103903 29.60320034598335 22.742999243161425 21 22.625290587662864 25.755527923447048 30.42114937557442 21.198032113315673 22 24.21342847875446 24.851335279489675 25.321656395285974 25.613579846469886 23 20.921180668180344 24.737809492918153 29.927559736187696 24.413450102713806 24 22.797059141528813 28.121959130716835 27.37593253324684 21.705049194507513 25 24.008000864958372 22.27267812736512 25.737917612714888 27.981403394961617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 73.0 1 73.0 2 71.0 3 69.0 4 69.0 5 69.0 6 90.0 7 111.0 8 111.0 9 111.0 10 115.0 11 119.0 12 119.0 13 119.0 14 101.0 15 83.0 16 83.0 17 83.0 18 135.0 19 187.0 20 187.0 21 187.0 22 285.5 23 384.0 24 384.0 25 384.0 26 484.0 27 584.0 28 584.0 29 584.0 30 732.0 31 880.0 32 880.0 33 880.0 34 900.0 35 920.0 36 920.0 37 920.0 38 925.0 39 930.0 40 930.0 41 930.0 42 1084.0 43 1238.0 44 1238.0 45 1238.0 46 2596.0 47 3954.0 48 3954.0 49 3954.0 50 3560.0 51 3166.0 52 3166.0 53 3166.0 54 2526.5 55 1887.0 56 1887.0 57 1887.0 58 1602.5 59 1318.0 60 1318.0 61 1318.0 62 1201.5 63 1085.0 64 1085.0 65 1085.0 66 893.0 67 701.0 68 701.0 69 701.0 70 591.0 71 481.0 72 481.0 73 481.0 74 348.0 75 215.0 76 215.0 77 215.0 78 153.5 79 92.0 80 92.0 81 92.0 82 54.0 83 16.0 84 16.0 85 16.0 86 10.0 87 4.0 88 4.0 89 4.0 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05946588820413017 2 0.08649583738782571 3 0.027029949183695532 4 0.0 5 0.0 6 0.3243593902043464 7 0.005405989836739107 8 0.01621796951021732 9 0.01621796951021732 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.005405989836739107 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 18498.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.00129743756082 #Duplication Level Percentage of deduplicated Percentage of total 1 92.06556805301247 57.08184668612824 2 5.083267939663441 6.3033841496377985 3 0.9329496904699626 1.7353227375932534 4 0.4446769552707298 1.1028219266947779 5 0.2964513035138199 0.9190182722456481 6 0.20054058767111343 0.7460265974699967 7 0.104629871828407 0.45410314628608495 8 0.061034091899904094 0.30273543085738996 9 0.06975324788560468 0.3892312682452157 >10 0.47083442322783153 6.081738566331495 >50 0.13950649577120935 5.584387501351498 >100 0.11334902781410759 13.255487079684288 >500 0.01743831197140117 6.0438966374743215 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 574 3.1030381662882474 No Hit CCCATGTACTCTGCGTTGATACCAC 544 2.940858471186074 No Hit GTATCAACGCAGAGTACTTTTTTTT 346 1.870472483511731 No Hit GAGTACATGGGAAGCAGTGGTATCA 249 1.3460914693480377 No Hit GGTATCAACGCAGAGTACTTTTTTT 240 1.2974375608173856 No Hit ACGCAGAGTACATGGGAAGCAGTGG 223 1.2055357335928207 No Hit CATGTACTCTGCGTTGATACCACTG 217 1.1730997945723862 No Hit TATCAACGCAGAGTACTTTTTTTTT 174 0.9406422315926046 No Hit GCGTTGATACCACTGCTTCCCATGT 169 0.913612282408909 No Hit GGTATCAACGCAGAGTACATGGGAA 161 0.8703643637149961 No Hit GTATCAACGCAGAGTACATGGGAAG 154 0.8325224348578224 No Hit ACTCTGCGTTGATACCACTGCTTCC 144 0.7784625364904314 No Hit TATCAACGCAGAGTACATGGGAAGC 138 0.7460265974699967 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 129 0.6973726889393448 No Hit GTGGTATCAACGCAGAGTACATGGG 108 0.5838469023678236 No Hit GATACCACTGCTTCCCATGTACTCT 82 0.44329116661260676 No Hit CAGTGGTATCAACGCAGAGTACATG 78 0.4216672072656503 No Hit GCTTCCCATGTACTCTGCGTTGATA 78 0.4216672072656503 No Hit ATACCACTGCTTCCCATGTACTCTG 73 0.39463725808195477 No Hit CATGGGAAGCAGTGGTATCAACGCA 71 0.3838252784084766 No Hit GCAGTGGTATCAACGCAGAGTACAT 69 0.37301329873499833 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 65 0.35138933938804195 No Hit CCACTGCTTCCCATGTACTCTGCGT 63 0.3405773597145637 No Hit GTACTCTGCGTTGATACCACTGCTT 63 0.3405773597145637 No Hit ACATGGGAAGCAGTGGTATCAACGC 62 0.33517136987782464 No Hit TACCACTGCTTCCCATGTACTCTGC 58 0.3135474105308682 No Hit GGGAAGCAGTGGTATCAACGCAGAG 57 0.3081414206941291 No Hit CTGCTTCCCATGTACTCTGCGTTGA 56 0.30273543085738996 No Hit AAGCAGTGGTATCAACGCAGAGTAC 55 0.2973294410206509 No Hit ATGGGAAGCAGTGGTATCAACGCAG 52 0.28111147151043353 No Hit GCAGAGTACATGGGAAGCAGTGGTA 51 0.27570548167369446 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 49 0.2648935020002163 No Hit CTTCCCATGTACTCTGCGTTGATAC 48 0.2594875121634771 No Hit CCATGTACTCTGCGTTGATACCACT 46 0.2486755324899989 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 46 0.2486755324899989 No Hit GTACTGGTTCACTATCGGTCAGTCA 45 0.2432695426532598 No Hit ATCAACGCAGAGTACTTTTTTTTTT 43 0.2324575629797816 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 42 0.22705157314304247 No Hit ATCAACGCAGAGTACATGGGAAGCA 42 0.22705157314304247 No Hit GTTGATACCACTGCTTCCCATGTAC 41 0.22164558330630338 No Hit TTCCCATGTACTCTGCGTTGATACC 35 0.18920964428586876 No Hit GTGGTATCAACGCAGAGTACTTTTT 34 0.18380365444912963 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 28 0.15136771542869498 No Hit TGATACCACTGCTTCCCATGTACTC 27 0.1459617255919559 No Hit GGATACCACGTGTCCCGCCCTACTC 23 0.12433776624499945 No Hit GTACATGGTAAGCAGTGGTATCAAC 22 0.11893177640826035 No Hit GTACATGGAAAGCAGTGGTATCAAC 19 0.10271380689804302 No Hit CTATCGGTCAGTCAGGAGTATTTAG 19 0.10271380689804302 No Hit TAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10271380689804302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGAT 140 9.061734E-5 8.121951 14 TACTCTG 145 1.3642006E-4 7.8418837 7 CCCATGT 135 5.1330804E-4 7.784153 1 CCATGTA 150 1.8370937E-4 7.642623 2 CTCTGCG 150 2.0222303E-4 7.5804877 9 GTTGATA 150 2.0222303E-4 7.5804877 15 TGCGTTG 150 2.0222303E-4 7.5804877 12 CTGCGTT 150 2.0222303E-4 7.5804877 11 TCTGCGT 150 2.0222303E-4 7.5804877 10 ACTCTGC 155 2.954678E-4 7.3359556 8 TGATACC 155 2.954678E-4 7.3359556 17 TTGATAC 155 2.954678E-4 7.3359556 16 GCGTTGA 155 2.954678E-4 7.3359556 13 GTACTCT 160 3.8721896E-4 7.164959 6 TGTACTC 160 3.8721896E-4 7.164959 5 ATGTACT 170 7.757069E-4 6.7434907 4 CATGTAC 170 7.757069E-4 6.7434907 3 AACGCAG 270 2.4557085E-6 6.3688526 6 GATACCA 185 0.0022223978 6.146342 18 GTATCAA 305 2.7076876E-6 5.9512234 1 >>END_MODULE