FastQCFastQC Report
Thu 26 May 2016
SRR1926990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667352
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11260.16872654910751747No Hit
GTACATGGGAAGCAGTGGTATCAAC10950.16408132439851833No Hit
CCCATGTACTCTGCGTTGATACCAC8840.13246382718565317No Hit
GTCCTACAGTGGACATTTCTAAATT8190.1227238398925904No Hit
GTCCTAAAGTGTGTATTTCTCATTT6840.1024946355146909No Hit
CTGTAGGACGTGGAATATGGCAAGA6780.10159555976456203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT500.001477130613.3247526
TATACCC655.3454234E-513.1793175
GCCTTAT603.9344266E-412.7237431
GGCGAGG1451.0004442E-1011.76989119
GTACTAG657.7161554E-411.7449941
CTTAATA751.9996762E-411.4427382
GTCTAGG600.005676437611.1332751
TGTAGGA4900.011.092452
GTAGGAC4400.011.0526453
GTATTAG700.001434612210.9060661
GTATTAA1158.2914084E-710.7875221
TAGGACA2300.010.7631654
GTATAGT803.6113153E-410.7356591
TCCTATA909.1417984E-510.5951272
ACAGACC909.389399E-510.5672498
GTCCTAG1002.276848E-510.4970881
TGGCAAG3100.010.39884718
ATTTCTC6200.010.39884715
CTGTAGG4850.010.2314631
GTCTTGG750.002547332310.1789941
Produced by FastQC (version 0.11.5)