##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926990_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 667352 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.256158668888382 31.0 28.0 31.0 16.0 34.0 2 28.402824896006905 31.0 28.0 31.0 18.0 34.0 3 28.183287080880856 31.0 28.0 33.0 16.0 34.0 4 31.804397079801962 35.0 32.0 37.0 19.0 37.0 5 31.578044270489936 35.0 32.0 37.0 19.0 37.0 6 31.33938611107781 35.0 32.0 37.0 17.0 37.0 7 31.10548406238387 35.0 32.0 37.0 16.0 37.0 8 30.90240982270226 35.0 32.0 37.0 11.0 37.0 9 33.13920989223079 37.0 33.0 39.0 17.0 39.0 10 33.35998843189201 37.0 34.0 39.0 17.0 39.0 11 33.48942836763807 38.0 34.0 39.0 19.0 39.0 12 33.41670362866973 38.0 34.0 39.0 17.0 39.0 13 33.499274745561564 38.0 34.0 39.0 18.0 39.0 14 34.525350939234464 39.0 34.0 41.0 18.0 41.0 15 34.119188973735 39.0 33.0 41.0 16.0 41.0 16 34.20088049485129 39.0 34.0 41.0 17.0 41.0 17 34.411126362099765 39.0 34.0 41.0 17.0 41.0 18 34.4580296455244 39.0 34.0 41.0 17.0 41.0 19 33.849655054603865 39.0 33.0 41.0 10.0 41.0 20 34.149540272599765 39.0 34.0 41.0 16.0 41.0 21 34.12041771059351 39.0 34.0 41.0 11.0 41.0 22 34.14035021997387 39.0 34.0 41.0 10.0 41.0 23 34.130605737302055 39.0 34.0 41.0 2.0 41.0 24 32.848880350999174 39.0 32.0 41.0 2.0 41.0 25 33.40863742073149 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.10462926758187763 1101 2 -0.20471931009566902 1101 3 -0.9623755954321496 1101 4 -0.6058008462961908 1101 5 -0.4146843265063467 1101 6 -1.9652398307823482 1101 7 -0.5555738611105134 1101 8 -0.08932589291918447 1101 9 -0.2764142517212278 1101 10 -9.25694233594114 1101 11 -4.010207786577855 1101 12 -1.5001576080367265 1101 13 -0.6803746165220588 1101 14 -0.49735188955528997 1101 15 -2.1823738407024464 1101 16 -0.4908555237223595 1101 17 -0.3239032799269168 1101 18 -0.19750547377316252 1101 19 -1.090045239336476 1101 20 -0.31727547367896136 1101 21 -0.4190107435481778 1101 22 -0.2317498480629112 1101 23 -0.3697957859450014 1101 24 -0.5218380390877115 1101 25 -0.4721108109419063 1102 1 -0.0498273391580355 1102 2 -0.04709361818433422 1102 3 -0.24185420676291258 1102 4 -0.1270772792222452 1102 5 -0.08460244501768699 1102 6 -0.8647424198699198 1102 7 -0.2520103583173601 1102 8 0.18983641608777901 1102 9 0.37480121648273723 1102 10 0.6177254190776083 1102 11 0.2134740745042052 1102 12 0.29675589348101283 1102 13 0.25368145061565883 1102 14 -0.0153581645852654 1102 15 0.4501746887468343 1102 16 0.4582402087470925 1102 17 0.2582664519813562 1102 18 0.12370973934828555 1102 19 0.43086897641232014 1102 20 0.33979668916571626 1102 21 0.37891798639595464 1102 22 0.2758847717365924 1102 23 -0.3296406957733282 1102 24 -3.2288451558899958 1102 25 -1.4985271566297342 1103 1 0.11481668010527457 1103 2 -0.018542271054307946 1103 3 0.23601121039184392 1103 4 0.10710728409937431 1103 5 -0.45749324288310333 1103 6 0.5137139122919976 1103 7 0.27624162658437257 1103 8 0.20703282374103793 1103 9 0.16040059172678411 1103 10 0.4600786665069947 1103 11 0.10020581991615529 1103 12 -0.013060584457292634 1103 13 0.15971815502800268 1103 14 -0.03527226064149147 1103 15 0.4926216495694291 1103 16 0.47295841778002057 1103 17 0.1385319732621042 1103 18 0.26279603376452343 1103 19 -0.9370185675158282 1103 20 0.17855238355593173 1103 21 0.07936833131330445 1103 22 0.3518811273268554 1103 23 0.32979962701724475 1103 24 1.8978501454245915 1103 25 1.1083607777708906 1104 1 -0.5334659915241708 1104 2 -0.6314548020716764 1104 3 -1.8002646374162623 1104 4 -0.8157173643704354 1104 5 -0.7114190079782219 1104 6 -2.2825815798502695 1104 7 -0.8642401765587202 1104 8 -0.3950794591373743 1104 9 -0.3844443214245814 1104 10 -0.2544581407717459 1104 11 -0.5126291676768986 1104 12 -0.34795202366324673 1104 13 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1108 22 0.6791152226926087 1108 23 0.5551795262403232 1108 24 -0.6783456497450615 1108 25 0.3817869450208704 1109 1 0.8754760207646157 1109 2 0.672007179495143 1109 3 -0.9480180936374616 1109 4 0.5532611302532224 1109 5 0.9351074897176268 1109 6 1.0138604324385199 1109 7 1.0300877804305273 1109 8 1.3560438127520271 1109 9 0.7750526063788001 1109 10 0.8387599851883181 1109 11 0.7637955635058944 1109 12 0.41624344484673514 1109 13 0.708436103745953 1109 14 0.8015042961350645 1109 15 -0.5274516105038316 1109 16 0.48035768517929256 1109 17 0.43237812710825807 1109 18 0.4875579385264288 1109 19 -1.089619300116567 1109 20 0.0468307718343155 1109 21 0.3545331664781415 1109 22 0.6357639112096436 1109 23 0.427308784526403 1109 24 -0.26163703406258776 1109 25 0.32037542978554256 1110 1 0.2169980075497584 1110 2 0.3155615198915882 1110 3 0.5165068550980152 1110 4 0.4260521988337622 1110 5 0.39330297843943285 1110 6 0.5362436632428 1110 7 0.5946172860126637 1110 8 0.8572058086508676 1110 9 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2216 12 -2.2995709839290654 2216 13 -1.8029229806603446 2216 14 -2.0999140566137626 2216 15 -3.0072881116278865 2216 16 -2.087085601148118 2216 17 -2.3053901132350916 2216 18 -2.0341996743899813 2216 19 -1.547305791021941 2216 20 -2.345321282120313 2216 21 -4.806406952058541 2216 22 -3.277536722348504 2216 23 -3.3358017815884544 2216 24 -3.3005211542342607 2216 25 -2.994564280003953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38092.0 3 2.0 4 301.0 5 1840.0 6 2352.0 7 1832.0 8 1457.0 9 1295.0 10 1099.0 11 1279.0 12 1132.0 13 1129.0 14 1112.0 15 1281.0 16 1286.0 17 1388.0 18 1588.0 19 2036.0 20 2711.0 21 3414.0 22 4173.0 23 5148.0 24 5968.0 25 6893.0 26 7871.0 27 8509.0 28 9545.0 29 11672.0 30 13596.0 31 16706.0 32 21389.0 33 28255.0 34 38076.0 35 53641.0 36 78058.0 37 123541.0 38 167685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93790147320108 19.036419260801196 12.94025943598131 25.085419830016413 2 14.20843905535157 20.109565773224958 41.88062402374375 23.801371147679717 3 18.475398496195474 24.886149303091187 30.180675616669394 26.457776584043945 4 11.73843738425388 17.45875126898986 38.79427593725304 32.00853540950321 5 12.564224776224991 38.912270604387366 35.691888942846695 12.831615676540945 6 31.514302200972715 37.45715334867094 18.525490666286974 12.503053784069365 7 27.071779194584472 32.56982081503458 22.553378242297992 17.805021748082954 8 24.394840986531314 37.08510628704039 20.937562469845382 17.582490256582915 9 26.13808380469352 15.794088288985009 20.563144279680703 37.50468362664077 10 14.291817763433059 29.656091517044015 34.91148565286545 21.14060506665747 11 33.527865452114824 23.46165259381154 24.356380443677107 18.65410151039653 12 22.577386643965987 25.567342191794783 31.92193443379684 19.933336730442395 13 28.205873713364227 22.530288769925242 25.9064150445714 23.357422472139127 14 20.36790856539939 22.72945951252265 28.01818260842197 28.884449313655992 15 22.974700523194556 31.012203876525486 23.62243666887974 22.390658931400218 16 21.611186882690607 28.928823009247463 27.975300130393727 21.4846899776682 17 21.365902203464515 29.043256966946533 28.01796103845497 21.572879791133985 18 21.253149133679134 28.497093280299502 29.454609762202004 20.795147823819367 19 23.035486249752694 27.689047165116882 27.745852743154853 21.529613841975575 20 22.89076995476849 28.067064927746486 28.293072595221467 20.749092522263552 21 22.578629171250185 27.482217952599193 27.499752714890757 22.439400161259865 22 22.429896737257017 28.391409259175997 27.68085967357583 21.497834329991157 23 21.980159735263307 28.040747629374625 27.857901859781276 22.1211907755808 24 22.6038124384589 27.758502601024826 28.105455782578005 21.532229177938277 25 22.266534180406122 27.738786252002654 28.012751053220807 21.981928514370413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 298.0 2 356.0 3 414.0 4 414.0 5 414.0 6 619.5 7 825.0 8 825.0 9 825.0 10 1245.0 11 1665.0 12 1665.0 13 1665.0 14 2763.0 15 3861.0 16 3861.0 17 3861.0 18 6552.5 19 9244.0 20 9244.0 21 9244.0 22 14000.5 23 18757.0 24 18757.0 25 18757.0 26 26277.0 27 33797.0 28 33797.0 29 33797.0 30 43213.0 31 52629.0 32 52629.0 33 52629.0 34 59050.0 35 65471.0 36 65471.0 37 65471.0 38 70927.5 39 76384.0 40 76384.0 41 76384.0 42 79594.5 43 82805.0 44 82805.0 45 82805.0 46 85572.0 47 88339.0 48 88339.0 49 88339.0 50 83950.0 51 79561.0 52 79561.0 53 79561.0 54 70226.5 55 60892.0 56 60892.0 57 60892.0 58 51380.5 59 41869.0 60 41869.0 61 41869.0 62 33944.5 63 26020.0 64 26020.0 65 26020.0 66 20059.5 67 14099.0 68 14099.0 69 14099.0 70 10296.0 71 6493.0 72 6493.0 73 6493.0 74 4528.0 75 2563.0 76 2563.0 77 2563.0 78 1738.5 79 914.0 80 914.0 81 914.0 82 621.0 83 328.0 84 328.0 85 328.0 86 209.5 87 91.0 88 91.0 89 91.0 90 58.5 91 26.0 92 26.0 93 26.0 94 16.0 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22821539457437753 2 0.13531090039439456 3 0.13620997614452343 4 0.07237559788537384 5 0.024574737170189046 6 0.022027355878157254 7 0.30059099245975135 8 0.30433714141862167 9 0.02007935841954471 10 0.04270609813112121 11 0.01648305541902924 12 0.01768182308586773 13 0.016183363502319616 14 0.018131360960932163 15 0.01573382562725518 16 0.021278126086383197 17 0.01947997458612546 18 0.01573382562725518 19 0.024574737170189046 20 0.01768182308586773 21 0.015883671585609993 22 0.01828120691928697 23 0.018431052877641782 24 0.01723228521080329 25 0.016932593294093672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 667352.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.61611751787946 #Duplication Level Percentage of deduplicated Percentage of total 1 93.03133381996331 80.58012942995018 2 5.427908849184517 9.40288781514608 3 0.8357158718493531 2.1715939250298204 4 0.2702921752729815 0.9364663527043136 5 0.1206679505976162 0.52258946948024 6 0.0687786037671933 0.3574401375968929 7 0.05152396094140128 0.3123963819120932 8 0.02989635926133153 0.2071605253708989 9 0.02141023312517504 0.16690241416098214 >10 0.12014012123665395 1.9424123060048943 >50 0.010214021823217777 0.6345804234955876 >100 0.010386885411262679 1.6282558079613054 >500 0.0013849180527396305 0.8041565420615145 >1k 3.4622951318490764E-4 0.3330284691252329 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1126 0.16872654910751747 No Hit GTACATGGGAAGCAGTGGTATCAAC 1095 0.16408132439851833 No Hit CCCATGTACTCTGCGTTGATACCAC 884 0.13246382718565317 No Hit GTCCTACAGTGGACATTTCTAAATT 819 0.1227238398925904 No Hit GTCCTAAAGTGTGTATTTCTCATTT 684 0.1024946355146909 No Hit CTGTAGGACGTGGAATATGGCAAGA 678 0.10159555976456203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.4984595835481127E-4 0.0 4 0.0 0.0 0.0 1.4984595835481127E-4 0.0 5 0.0 0.0 0.0 1.4984595835481127E-4 0.0 6 0.0 0.0 0.0 1.4984595835481127E-4 0.0 7 0.0 0.0 0.0 1.4984595835481127E-4 0.0 8 0.0 0.0 0.0 1.4984595835481127E-4 0.0 9 0.0 0.0 0.0 1.4984595835481127E-4 0.0 10 0.0 0.0 0.0 1.4984595835481127E-4 0.0 11 0.0 0.0 0.0 2.9969191670962253E-4 0.0 12 0.0 0.0 0.0 2.9969191670962253E-4 0.0 13 0.0 0.0 0.0 4.4953787506443374E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGT 50 0.0014771306 13.324752 6 TATACCC 65 5.3454234E-5 13.179317 5 GCCTTAT 60 3.9344266E-4 12.723743 1 GGCGAGG 145 1.0004442E-10 11.769891 19 GTACTAG 65 7.7161554E-4 11.744994 1 CTTAATA 75 1.9996762E-4 11.442738 2 GTCTAGG 60 0.0056764376 11.133275 1 TGTAGGA 490 0.0 11.09245 2 GTAGGAC 440 0.0 11.052645 3 GTATTAG 70 0.0014346122 10.906066 1 GTATTAA 115 8.2914084E-7 10.787522 1 TAGGACA 230 0.0 10.763165 4 GTATAGT 80 3.6113153E-4 10.735659 1 TCCTATA 90 9.1417984E-5 10.595127 2 ACAGACC 90 9.389399E-5 10.567249 8 GTCCTAG 100 2.276848E-5 10.497088 1 TGGCAAG 310 0.0 10.398847 18 ATTTCTC 620 0.0 10.398847 15 CTGTAGG 485 0.0 10.231463 1 GTCTTGG 75 0.0025473323 10.178994 1 >>END_MODULE