FastQCFastQC Report
Thu 26 May 2016
SRR1926990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667352
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18290.2740682578309498No Hit
GTACATGGGAAGCAGTGGTATCAAC13030.19524928373631906No Hit
GGTATCAACGCAGAGTACTTTTTTT12300.18431052877641785No Hit
CCCATGTACTCTGCGTTGATACCAC10870.16288255673167984No Hit
TATCAACGCAGAGTACTTTTTTTTT10270.15389179923039117No Hit
GTCCTACAGTGGACATTTCTAAATT8570.12841798631007326No Hit
CTGTAGGACGTGGAATATGGCAAGA7540.11298385259952769No Hit
GTCCTAAAGTGTGTATTTCTCATTT6970.10444263297330345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA653.2505322E-614.6642541
GTCTAGG400.005159355214.2976471
TGCTATA551.9040564E-413.8633422
TAGGACC2300.013.6616134
CTAGGAC1153.601599E-1013.2506143
AATCCCG957.475137E-812.98482419
TAGGACT1102.6429916E-912.9841794
AGGACGT4500.012.9072365
GGACGTG4500.012.9072366
GACGTGG2300.012.7913167
GTAGGAC5200.012.6374553
GACGTGA2450.012.3955357
TAGGACA2000.012.378254
GTCCTAA2700.012.3559921
CTGTAGG5600.012.2551261
TGTAGGA5900.012.1157392
ACGTGGA2400.011.8602498
TTAGGAC3150.011.7914713
GTATGAG657.791525E-411.7314031
TAGGACG5150.011.2781674
Produced by FastQC (version 0.11.5)