FastQCFastQC Report
Thu 26 May 2016
SRR1926989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518724
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13240.2552417085000887No Hit
GTCCTACAGTGGACATTTCTAAATT9400.18121390180519892No Hit
CTGTAGGACGTGGAATATGGCAAGA7700.14844117488298209No Hit
GGTATCAACGCAGAGTACTTTTTTT7370.14207941024514No Hit
GTCCTAAAGTGTGTATTTCTCATTT7250.1397660412859247No Hit
TATCAACGCAGAGTACTTTTTTTTT6650.12819919648984818No Hit
GTCCTACAGTGTGCATTTCTCATTT6100.11759625542677801No Hit
CTTTAGGACGTGAAATATGGCGAGG5850.11277673676174613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT307.8014756E-418.96789410
ATGTCGA400.00533572914.22454917
GGTATCA2500.013.7376771
TAGGACC1600.013.6859554
TGTACTG852.626366E-713.4370365
GTCTTAG751.41164055E-512.7200731
GGCATGC701.0779575E-412.2266538
TCCAACG701.1065105E-412.19247118
GTCCCAG550.002968622612.1418881
TTATGAC550.002990264712.1301233
AGGACAC550.00302079112.113695
GTCCTAT802.73722E-511.9250681
AGGCACT802.8031674E-511.8973766
GTCCTAC6050.011.8265131
GTATCAA6650.011.6202161
CTAAATT3300.011.49458519
TGTAGGA4600.011.1883432
GGGGCAT855.404295E-511.15650811
GGGCATA855.404295E-511.15650812
GTCTAGG600.00568573811.1300631
Produced by FastQC (version 0.11.5)