##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926989_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 518724 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.38987978192642 31.0 28.0 31.0 22.0 34.0 2 28.53179918415188 31.0 29.0 31.0 22.0 34.0 3 28.301696085008597 31.0 28.0 33.0 16.0 34.0 4 31.919307762895105 35.0 32.0 37.0 19.0 37.0 5 31.704147099420886 35.0 32.0 37.0 19.0 37.0 6 31.420686145233304 35.0 32.0 37.0 17.0 37.0 7 31.2186557784101 35.0 32.0 37.0 17.0 37.0 8 31.03630639800742 35.0 32.0 37.0 13.0 37.0 9 33.209982572620504 37.0 33.0 39.0 17.0 39.0 10 33.428700040869515 37.0 34.0 39.0 17.0 39.0 11 33.575915130204116 38.0 34.0 39.0 19.0 39.0 12 33.48246851890408 38.0 34.0 39.0 18.0 39.0 13 33.56984446449364 38.0 34.0 39.0 19.0 39.0 14 34.59583130913549 39.0 34.0 41.0 19.0 41.0 15 34.19360777600419 39.0 34.0 41.0 17.0 41.0 16 34.27301609333673 39.0 34.0 41.0 17.0 41.0 17 34.485439270209206 39.0 34.0 41.0 18.0 41.0 18 34.54338337921515 39.0 34.0 41.0 18.0 41.0 19 33.95539824646633 39.0 34.0 41.0 11.0 41.0 20 34.26017303999815 39.0 34.0 41.0 18.0 41.0 21 34.24032047871315 39.0 34.0 41.0 16.0 41.0 22 34.25255048927753 39.0 34.0 41.0 11.0 41.0 23 34.251515256668284 39.0 34.0 41.0 10.0 41.0 24 32.95838056461625 39.0 32.0 41.0 2.0 41.0 25 33.548177836383125 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7442954292396635 1101 2 -0.9261771289150751 1101 3 -1.5250101575491115 1101 4 -0.7986691874665794 1101 5 -0.9385345733754455 1101 6 -2.5444887423775278 1101 7 -1.520056650142255 1101 8 -0.8429740808255453 1101 9 -0.8171860433397384 1101 10 -9.689636745708818 1101 11 -4.780426659122998 1101 12 -2.279508482575949 1101 13 -1.5620684487685033 1101 14 -1.6044903156330932 1101 15 -3.2734302579116026 1101 16 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24 0.5786853516560981 2211 25 0.2528974858039703 2212 1 -0.3254687148523452 2212 2 0.17104146072556503 2212 3 -2.5319464764821262 2212 4 -0.9925832750003245 2212 5 0.0981154981290544 2212 6 0.8822898842772062 2212 7 0.9826023995744002 2212 8 0.7678364819569374 2212 9 0.45873873545516375 2212 10 0.9033474009404117 2212 11 0.6554372452521591 2212 12 0.6333416791695257 2212 13 0.6120476867764992 2212 14 0.532579605826669 2212 15 -0.38245359055665773 2212 16 0.39075004149260906 2212 17 0.5505364174054321 2212 18 0.3603830086167008 2212 19 1.441156524372019 2212 20 -0.11124917872717077 2212 21 0.049853587378116515 2212 22 0.7290633144840726 2212 23 0.7691517167578183 2212 24 1.947913681999026 2212 25 1.4290563593925327 2213 1 -0.58242473288621 2213 2 -0.4932760580829729 2213 3 0.21733817100611574 2213 4 -6.727167253895679 2213 5 -1.3337019857842378 2213 6 -3.5771897578371643 2213 7 -1.373337468940317 2213 8 -3.0038802244903096 2213 9 -1.1376363465486605 2213 10 -0.757423365219573 2213 11 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-0.09173891667006018 2214 23 -0.4683322194792936 2214 24 -1.286681511176269 2214 25 -0.6953988397981306 2215 1 0.4196892450898382 2215 2 0.01494675358811648 2215 3 0.29389150273186004 2215 4 0.6190830475270346 2215 5 0.6669571252197031 2215 6 1.2616415258217089 2215 7 0.7772613575205796 2215 8 -0.5081081804424024 2215 9 0.14743720059385623 2215 10 -0.2110926844061325 2215 11 -0.02831937942951157 2215 12 -0.48065790786330354 2215 13 -0.0796929698412967 2215 14 -0.17371126071610377 2215 15 0.044960546686098724 2215 16 0.27892541488375855 2215 17 -0.011229705521124345 2215 18 -0.6214670843009102 2215 19 0.3075134656618559 2215 20 -3.6740546023626592 2215 21 -1.802827257828021 2215 22 -1.0555398928942736 2215 23 -1.0001854709357616 2215 24 -0.7874294248906466 2215 25 -0.5842824801697191 2216 1 0.023659488811837548 2216 2 -0.5126367133196688 2216 3 0.2144153885839266 2216 4 0.1450499210628564 2216 5 0.4641828865940809 2216 6 -0.9626566945555908 2216 7 -9.228859777382766 2216 8 -10.208301349650409 2216 9 -5.0721085092687375 2216 10 -2.6475550849102945 2216 11 -1.4335623479333393 2216 12 -1.1154653551527716 2216 13 -1.1882335148250647 2216 14 -1.1435617943289174 2216 15 -2.152267954677047 2216 16 -1.2699395447699047 2216 17 -1.4944472743290973 2216 18 -1.2680126434641181 2216 19 -0.5066070664439621 2216 20 -1.439380044528349 2216 21 -3.968955516702163 2216 22 -2.595861567575408 2216 23 -2.168641350266654 2216 24 -3.017725480676816 2216 25 -2.5394918838111664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28075.0 3 3.0 4 182.0 5 1365.0 6 1881.0 7 1591.0 8 1239.0 9 1033.0 10 913.0 11 961.0 12 927.0 13 902.0 14 878.0 15 950.0 16 1024.0 17 1123.0 18 1221.0 19 1604.0 20 2091.0 21 2612.0 22 3262.0 23 3938.0 24 4597.0 25 5313.0 26 6134.0 27 6608.0 28 7397.0 29 8851.0 30 10554.0 31 12713.0 32 16794.0 33 21916.0 34 29630.0 35 41652.0 36 60724.0 37 95895.0 38 132171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26688019039413 19.626861490969993 13.168026302826355 24.938232015809515 2 13.505125389157065 20.75826035934662 43.27368670950209 22.462927541994222 3 18.014842837703508 25.789478763547223 30.392109555197404 25.80356884355186 4 10.934084906988902 17.968706294043546 40.25606747186904 30.841141327098516 5 11.71145287806684 39.79352579577796 36.279530747456675 12.215490578698526 6 30.081427791885755 38.60464040075662 19.401911615270556 11.912020192087063 7 26.431189964268587 33.10453371281845 23.15194357221303 17.312332750699934 8 23.554509057939494 38.07485679930251 21.554048959849677 16.816585182908323 9 26.096194819092428 15.951295226893672 21.076479605117278 36.87603034889662 10 13.508687007384292 30.557553714035556 35.87100531692293 20.062753961657215 11 33.00902735198961 23.610709424154432 25.283719531240962 18.096543692615004 12 22.110134197130957 26.149352151781585 33.004010488971154 18.736503162116307 13 27.933512386894517 23.439563638887655 26.2790343447353 22.347889629482527 14 19.54569731278476 23.61305797266317 29.29926480937681 27.541979905175257 15 22.054244359909227 32.3559296870933 24.2171548222029 21.372671130794576 16 20.468577069764034 29.65385994747741 29.362903319103644 20.514659663654907 17 20.042380397812725 29.99560385318412 29.0639292297488 20.898086519254353 18 19.887362722112538 29.088451690523197 31.0448543928924 19.979331194471868 19 22.216094684596786 28.18189531166242 28.796620170225566 20.805389833515232 20 22.140835402816553 28.69055698662677 29.61835387391834 19.550253736638336 21 22.071103690150025 27.85071271433006 28.25869418934891 21.81948940617101 22 21.501797954284722 29.53696651852423 28.870614775038806 20.090620752152244 23 20.913574629225444 28.99252666568976 29.127108382628546 20.96679032245625 24 21.747488205023398 28.18069113646311 29.772506859113896 20.2993137993996 25 21.32238957913977 28.601837069942853 29.432644624912275 20.643128726005106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 234.0 3 275.0 4 275.0 5 275.0 6 524.0 7 773.0 8 773.0 9 773.0 10 1132.0 11 1491.0 12 1491.0 13 1491.0 14 2475.5 15 3460.0 16 3460.0 17 3460.0 18 5896.0 19 8332.0 20 8332.0 21 8332.0 22 12640.0 23 16948.0 24 16948.0 25 16948.0 26 23506.5 27 30065.0 28 30065.0 29 30065.0 30 38565.0 31 47065.0 32 47065.0 33 47065.0 34 52910.5 35 58756.0 36 58756.0 37 58756.0 38 62031.0 39 65306.0 40 65306.0 41 65306.0 42 67130.5 43 68955.0 44 68955.0 45 68955.0 46 68110.5 47 67266.0 48 67266.0 49 67266.0 50 62889.0 51 58512.0 52 58512.0 53 58512.0 54 49496.0 55 40480.0 56 40480.0 57 40480.0 58 33190.0 59 25900.0 60 25900.0 61 25900.0 62 20005.5 63 14111.0 64 14111.0 65 14111.0 66 10445.0 67 6779.0 68 6779.0 69 6779.0 70 4738.0 71 2697.0 72 2697.0 73 2697.0 74 1823.5 75 950.0 76 950.0 77 950.0 78 619.0 79 288.0 80 288.0 81 288.0 82 187.5 83 87.0 84 87.0 85 87.0 86 57.0 87 27.0 88 27.0 89 27.0 90 15.5 91 4.0 92 4.0 93 4.0 94 3.0 95 2.0 96 2.0 97 2.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20454037214395326 2 0.11894572065298695 3 0.12125908961220225 4 0.06650935757744002 5 0.02159144361934285 6 0.020241978393133922 7 0.295340103793154 8 0.2781826173456404 9 0.017157486447513512 10 0.03682112260084361 11 0.015036898234899485 12 0.016193582714507136 13 0.013687433008690556 14 0.01600080196790586 15 0.013687433008690556 16 0.01735026719411479 17 0.016964705700912238 18 0.013109090768886729 19 0.022748128098950503 20 0.014651336741696932 21 0.014072994501893105 22 0.015229678981500759 23 0.015422459728102036 24 0.013687433008690556 25 0.013880213755291831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 518724.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.20130676743818 #Duplication Level Percentage of deduplicated Percentage of total 1 94.67270961102813 83.50256702906886 2 4.232286820532561 7.465864563711559 3 0.5214560547361125 1.3797931634855365 4 0.18023707176085316 0.6358858102897512 5 0.0950062211247838 0.41898364271210603 6 0.0588447491152348 0.31141102610194565 7 0.04062743903534158 0.25083752494720885 8 0.028026433597253465 0.19775744538468712 9 0.017995701276982117 0.1428519931943651 >10 0.129148833335102 2.213889091181393 >50 0.01161145672466895 0.6884962160424365 >100 0.010296937517280053 1.5634623962072551 >500 0.0015335864387383655 0.9723572831167567 >1k 2.1908377696262368E-4 0.2558428145561577 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1324 0.2552417085000887 No Hit GTCCTACAGTGGACATTTCTAAATT 940 0.18121390180519892 No Hit CTGTAGGACGTGGAATATGGCAAGA 770 0.14844117488298209 No Hit GGTATCAACGCAGAGTACTTTTTTT 737 0.14207941024514 No Hit GTCCTAAAGTGTGTATTTCTCATTT 725 0.1397660412859247 No Hit TATCAACGCAGAGTACTTTTTTTTT 665 0.12819919648984818 No Hit GTCCTACAGTGTGCATTTCTCATTT 610 0.11759625542677801 No Hit CTTTAGGACGTGAAATATGGCGAGG 585 0.11277673676174613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.9278074660127544E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCCGT 30 7.8014756E-4 18.967894 10 ATGTCGA 40 0.005335729 14.224549 17 GGTATCA 250 0.0 13.737677 1 TAGGACC 160 0.0 13.685955 4 TGTACTG 85 2.626366E-7 13.437036 5 GTCTTAG 75 1.41164055E-5 12.720073 1 GGCATGC 70 1.0779575E-4 12.226653 8 TCCAACG 70 1.1065105E-4 12.192471 18 GTCCCAG 55 0.0029686226 12.141888 1 TTATGAC 55 0.0029902647 12.130123 3 AGGACAC 55 0.003020791 12.11369 5 GTCCTAT 80 2.73722E-5 11.925068 1 AGGCACT 80 2.8031674E-5 11.897376 6 GTCCTAC 605 0.0 11.826513 1 GTATCAA 665 0.0 11.620216 1 CTAAATT 330 0.0 11.494585 19 TGTAGGA 460 0.0 11.188343 2 GGGGCAT 85 5.404295E-5 11.156508 11 GGGCATA 85 5.404295E-5 11.156508 12 GTCTAGG 60 0.005685738 11.130063 1 >>END_MODULE