FastQCFastQC Report
Thu 26 May 2016
SRR1926989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518724
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21080.4063818138354886No Hit
GGTATCAACGCAGAGTACTTTTTTT12520.24136149474479682No Hit
TATCAACGCAGAGTACTTTTTTTTT10420.20087753795852903No Hit
CTGTAGGACGTGGAATATGGCAAGA8930.17215320671493894No Hit
GTCCTACAGTGGACATTTCTAAATT8740.16849037252951474No Hit
GTCCTAAAGTGTGTATTTCTCATTT7520.14497112144415913No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6900.13301871515488004No Hit
CTTTAGGACGTGAAATATGGCGAGG6540.12607860827723413No Hit
GTCCTACAGTGTGCATTTCTCATTT5990.115475667214164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA250.006073969418.9743617
AAAGGCG350.002132863716.3251085
AAGGCGT350.002132863716.3251086
TAGGGTG551.0999278E-515.5830585
AGGCGTG400.005306683514.23625857
TAGGACC2350.014.1831614
TCCAACG500.001512512313.28205218
GGTATCA2700.013.0639141
TTAGGAC3050.012.4903623
TCTTAGG550.002986997612.1318942
ATTAAAC550.003006562112.1213283
TCAAGAC550.003006562112.1213283
AAGTGCC550.00308592412.0792497
AGGACCT3800.012.0290285
GGCGAGG2250.011.80626819
TTAGGAT657.84686E-411.7205924
GGACCTG3950.011.5722296
GTATCAA6800.011.495831
TTTAGGA3450.011.3281262
TAGGACA2300.011.1791514
Produced by FastQC (version 0.11.5)