FastQCFastQC Report
Thu 26 May 2016
SRR1926988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577221
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16900.2927821406359089No Hit
GGTATCAACGCAGAGTACTTTTTTT9950.17237765084776888No Hit
TATCAACGCAGAGTACTTTTTTTTT8470.146737558058352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC655.2173018E-513.2127761
TAGGACC1457.2759576E-1212.476844
CTCGAAC854.010628E-612.27213518
CTAATAC701.0552007E-412.2551133
CTAGGAC550.002988753612.1313233
GGTCAAG550.003107792512.06824311
GGATATA802.7311235E-511.9282011
CTGGACT907.250206E-611.6377544
CCTCGAA907.587376E-611.59034817
ACTGGGC752.0522515E-411.4102728
TGTAGGA2900.011.17515852
GCCTCGA855.4068507E-511.15648616
CCCTGTA951.3067092E-511.0367672
TCGAACT951.383145E-510.9803319
CTGTAGG2700.010.9562741
GTATCAA9900.010.8920141
GTAGGAC2650.010.7906653
GGCCTCG803.8256784E-410.66838815
TCTATAC1105.7874786E-610.3982773
GTCCTAC2750.010.0630641
Produced by FastQC (version 0.11.5)