##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577221 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.116645097804827 31.0 28.0 31.0 16.0 34.0 2 28.160032292657405 31.0 28.0 31.0 16.0 34.0 3 27.94187148423221 31.0 28.0 33.0 16.0 34.0 4 31.44291008123405 35.0 32.0 37.0 17.0 37.0 5 31.715483324411274 35.0 32.0 37.0 19.0 37.0 6 30.89940594676909 35.0 32.0 37.0 11.0 37.0 7 30.93303258197467 35.0 32.0 37.0 11.0 37.0 8 30.73430800334707 35.0 32.0 37.0 10.0 37.0 9 32.86512618217286 37.0 33.0 39.0 17.0 39.0 10 33.05401224141187 37.0 33.0 39.0 17.0 39.0 11 33.20333113313618 38.0 33.0 39.0 17.0 39.0 12 33.08372876246706 38.0 33.0 39.0 16.0 39.0 13 33.17356090648123 38.0 33.0 39.0 17.0 39.0 14 34.17989123749829 39.0 34.0 41.0 17.0 41.0 15 33.75129976213616 39.0 33.0 41.0 10.0 41.0 16 33.81747545567469 39.0 33.0 41.0 11.0 41.0 17 34.055708298901116 39.0 34.0 41.0 11.0 41.0 18 34.10941043378533 39.0 34.0 41.0 11.0 41.0 19 33.544178053120035 39.0 33.0 41.0 10.0 41.0 20 33.80782923698202 39.0 34.0 41.0 10.0 41.0 21 33.78834796377817 39.0 34.0 41.0 2.0 41.0 22 33.79913585957545 39.0 34.0 41.0 2.0 41.0 23 33.78745575784665 39.0 34.0 41.0 2.0 41.0 24 32.5491189682981 39.0 32.0 41.0 2.0 41.0 25 33.11568012944782 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.0018343283454562709 1101 2 -0.0071455600130185815 1101 3 -0.8846916781142156 1101 4 -0.2037218904313427 1101 5 0.07562042055723239 1101 6 -1.9804578463682745 1101 7 -0.5578139114902925 1101 8 0.08830782482244359 1101 9 -0.07281464271286353 1101 10 -8.51039637132481 1101 11 -3.7020659834515577 1101 12 -1.313651100808329 1101 13 -0.8489377796088675 1101 14 -0.47036111059889407 1101 15 -1.6745522404876567 1101 16 -0.27834202170954825 1101 17 -0.09502788180582655 1101 18 0.05638706484411671 1101 19 -0.8611633678908035 1101 20 0.16890961525466253 1101 21 0.11220408296276929 1101 22 0.19312659714082514 1101 23 0.09829474618325662 1101 24 -0.2006447957767037 1101 25 0.1607906147044318 1102 1 -0.42236293919359724 1102 2 -0.5967864201041024 1102 3 -0.6434280590446342 1102 4 -0.8835599027677681 1102 5 -0.5291267979819949 1102 6 -1.6592178625093936 1102 7 -0.8368362208233187 1102 8 -0.1747751290024233 1102 9 -0.028464034538536964 1102 10 -0.0993195280666086 1102 11 -0.3626902988951528 1102 12 -0.2787142241149496 1102 13 -0.519811705936192 1102 14 -0.792543620542979 1102 15 -0.07861346144805736 1102 16 -0.33638087569006103 1102 17 -0.3891392555303952 1102 18 -0.6089152929122648 1102 19 -0.16048025265474308 1102 20 -0.4486957343735156 1102 21 -0.5251914321118818 1102 22 -0.7500278387846961 1102 23 -0.9958310392551439 1102 24 -3.916010565073986 1102 25 -2.1511624607553017 1103 1 -0.1246455985824717 1103 2 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0.3955487755772005 2215 2 0.24874015292671814 2215 3 0.5368466747105742 2215 4 0.8585942362454553 2215 5 0.7491697498391723 2215 6 1.6313748215467854 2215 7 0.9663484270132834 2215 8 0.20481019795132127 2215 9 0.4028911128607504 2215 10 0.12066040710857351 2215 11 0.12044753101687178 2215 12 -0.1261315210229128 2215 13 0.3294320045129524 2215 14 0.1623362477494581 2215 15 0.19780522456316163 2215 16 0.5112550763601504 2215 17 0.05937388950227529 2215 18 -0.7689965811769142 2215 19 0.3431082640916685 2215 20 -3.268266655812006 2215 21 -2.0848456997457774 2215 22 -0.9430874673281906 2215 23 -0.5429576534445815 2215 24 -0.4858901761250749 2215 25 -0.4674278309255939 2216 1 -0.7630444135796282 2216 2 -1.1429593802095646 2216 3 -0.4605666348290818 2216 4 -0.661713221464094 2216 5 -0.8295320028131563 2216 6 -1.497201132694947 2216 7 -9.940868826890245 2216 8 -10.715753216170068 2216 9 -5.718866441583273 2216 10 -3.54868789221511 2216 11 -2.433394866874245 2216 12 -1.8626940516005952 2216 13 -1.7688680457676575 2216 14 -1.955607149262967 2216 15 -2.6970724501668144 2216 16 -1.7610255805235937 2216 17 -2.2360920393278896 2216 18 -1.9570761039858127 2216 19 -1.1279251623431463 2216 20 -2.3766693308568385 2216 21 -5.388194737293478 2216 22 -3.655469770490253 2216 23 -3.5159039713827447 2216 24 -3.3731250040229668 2216 25 -2.7306705719649393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35660.0 3 5.0 4 256.0 5 1712.0 6 2394.0 7 1948.0 8 1533.0 9 1400.0 10 1272.0 11 1197.0 12 1161.0 13 1154.0 14 1114.0 15 1191.0 16 1153.0 17 1353.0 18 1479.0 19 1838.0 20 2399.0 21 3018.0 22 3844.0 23 4537.0 24 5325.0 25 5952.0 26 6909.0 27 7571.0 28 8266.0 29 9921.0 30 11579.0 31 14241.0 32 17927.0 33 24053.0 34 32258.0 35 44776.0 36 65652.0 37 105799.0 38 145374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3353354761297 19.71841502527423 13.384315911391722 24.561933587204347 2 13.554499574079761 21.36159153641835 43.18050517089724 21.903403718604647 3 18.133018728801062 26.364322458079236 30.240551020585198 25.262107792534504 4 11.195328807306366 18.035165703903523 40.558448853352566 30.211056635437544 5 11.87950851061249 38.958903350008576 36.709391564417444 12.452196574961489 6 28.658760578609204 38.62881817614727 20.725236870741696 11.987184374501826 7 26.192420777523683 32.59955375450937 23.527806932792092 17.68021853517485 8 23.445696007450305 37.372968671542026 22.647672561928044 16.533662759079622 9 25.89270862212365 16.527758525090103 21.41980870529526 36.15972414749099 10 13.229572658756087 30.972232090712314 35.741110127150435 20.057085123381157 11 32.493502443081404 24.611359462175557 24.827251620057524 18.06788647468552 12 21.611571823137407 26.908855258643694 32.766674868226346 18.71289804999255 13 27.312767697489708 23.824730050039506 26.457868400260594 22.404633852210193 14 19.727305333816183 23.66628604696697 29.676900658258383 26.92950796095847 15 22.38497711151078 31.503640326711093 24.650088539121818 21.461294022656315 16 20.47972941361977 30.544932829911435 28.63804046666447 20.337297289804322 17 20.221202979638445 29.929113160762892 28.562319252421954 21.287364607176706 18 19.689264606763963 29.671296913957796 30.292629087563483 20.346809391714764 19 21.659247296362977 28.641310845912184 29.483289521792848 20.216152335931987 20 21.772797604711567 28.56343338537218 30.024275167075874 19.639493842840388 21 22.180946176344204 28.198161983837654 28.73962130768058 20.88127053213756 22 21.082288361874035 29.113941597112603 29.508827315903375 20.294942725109983 23 20.959438877616616 28.91138722885178 29.064559223327883 21.064614670203717 24 21.009387637704414 28.659844544323583 29.86786614039526 20.46290167757675 25 21.228361082088842 28.631898853490384 29.385958072782653 20.75378199163812 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 250.0 1 250.0 2 343.5 3 437.0 4 437.0 5 437.0 6 741.0 7 1045.0 8 1045.0 9 1045.0 10 1555.5 11 2066.0 12 2066.0 13 2066.0 14 3315.5 15 4565.0 16 4565.0 17 4565.0 18 7624.0 19 10683.0 20 10683.0 21 10683.0 22 15484.0 23 20285.0 24 20285.0 25 20285.0 26 27336.0 27 34387.0 28 34387.0 29 34387.0 30 42902.5 31 51418.0 32 51418.0 33 51418.0 34 57657.5 35 63897.0 36 63897.0 37 63897.0 38 68370.5 39 72844.0 40 72844.0 41 72844.0 42 74738.5 43 76633.0 44 76633.0 45 76633.0 46 76439.0 47 76245.0 48 76245.0 49 76245.0 50 70735.0 51 65225.0 52 65225.0 53 65225.0 54 54957.0 55 44689.0 56 44689.0 57 44689.0 58 36211.0 59 27733.0 60 27733.0 61 27733.0 62 21058.5 63 14384.0 64 14384.0 65 14384.0 66 10462.0 67 6540.0 68 6540.0 69 6540.0 70 4576.5 71 2613.0 72 2613.0 73 2613.0 74 1739.0 75 865.0 76 865.0 77 865.0 78 569.5 79 274.0 80 274.0 81 274.0 82 182.5 83 91.0 84 91.0 85 91.0 86 63.0 87 35.0 88 35.0 89 35.0 90 20.5 91 6.0 92 6.0 93 6.0 94 6.0 95 6.0 96 6.0 97 6.0 98 5.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23180029832594448 2 0.14240646130338291 3 0.1448318754861656 4 0.07085674291129394 5 0.020442776683453998 6 0.019576557332460182 7 0.303696504458431 8 0.29001023871272874 9 0.017497630890075035 10 0.032569847597367384 11 0.014032753486099779 12 0.016458167668882456 13 0.013339778005304727 14 0.015938436058286168 15 0.012993290264907203 16 0.018017362500671322 17 0.018017362500671322 18 0.01282004639470844 19 0.022175215385441625 20 0.015245460577491116 21 0.013339778005304727 22 0.015938436058286168 23 0.015938436058286168 24 0.013686265745702251 25 0.013513021875503491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 577221.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.99522296999811 #Duplication Level Percentage of deduplicated Percentage of total 1 95.88536780428721 87.25110422911393 2 3.340121896923088 6.07870273514979 3 0.346395368728048 0.9456097143955033 4 0.13405720807017463 0.48794262156323887 5 0.07118566980766051 0.3238777948208366 6 0.044306612000630775 0.2419014022825555 7 0.03379798838784281 0.21528188425024142 8 0.020483879252394668 0.1491148127889737 9 0.01594718468636114 0.13060058636512542 >10 0.08889269541739601 1.5183045673947793 >50 0.01296249111794093 0.8289618213115472 >100 0.005718707048122763 1.1238365911901693 >500 5.71870704784776E-4 0.4116166431289342 >1k 1.9062356826159198E-4 0.2931445962443737 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1690 0.2927821406359089 No Hit GGTATCAACGCAGAGTACTTTTTTT 995 0.17237765084776888 No Hit TATCAACGCAGAGTACTTTTTTTTT 847 0.146737558058352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 3.464877403975254E-4 0.0 11 0.0 0.0 0.0 3.464877403975254E-4 0.0 12 0.0 0.0 0.0 3.464877403975254E-4 0.0 13 0.0 0.0 0.0 3.464877403975254E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTAC 65 5.2173018E-5 13.212776 1 TAGGACC 145 7.2759576E-12 12.47684 4 CTCGAAC 85 4.010628E-6 12.272135 18 CTAATAC 70 1.0552007E-4 12.255113 3 CTAGGAC 55 0.0029887536 12.131323 3 GGTCAAG 55 0.0031077925 12.068243 11 GGATATA 80 2.7311235E-5 11.928201 1 CTGGACT 90 7.250206E-6 11.637754 4 CCTCGAA 90 7.587376E-6 11.590348 17 ACTGGGC 75 2.0522515E-4 11.410272 8 TGTAGGA 290 0.0 11.1751585 2 GCCTCGA 85 5.4068507E-5 11.156486 16 CCCTGTA 95 1.3067092E-5 11.036767 2 TCGAACT 95 1.383145E-5 10.98033 19 CTGTAGG 270 0.0 10.956274 1 GTATCAA 990 0.0 10.892014 1 GTAGGAC 265 0.0 10.790665 3 GGCCTCG 80 3.8256784E-4 10.668388 15 TCTATAC 110 5.7874786E-6 10.398277 3 GTCCTAC 275 0.0 10.063064 1 >>END_MODULE