FastQCFastQC Report
Thu 26 May 2016
SRR1926988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577221
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26410.45753706119493226No Hit
GGTATCAACGCAGAGTACTTTTTTT16780.2907032141935238No Hit
TATCAACGCAGAGTACTTTTTTTTT13790.23890329700409374No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8230.1425797051735817No Hit
CACACACACACACACACACACACAC6850.11867205108615243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGCC250.006072550518.97556519
GACGTGG807.821654E-915.416317
TACTTCA508.8013236E-515.1791357
CTTAATC400.005182876314.2873823
TAGGACG2050.013.4742794
TTAGGAC1650.013.27716353
CTAGGAC1102.6266207E-912.9885293
AGGACGT1850.012.8715165
GGACGTG1850.012.8715166
CACTGAC604.1327055E-412.64928157
GGCGAGG1155.3951226E-912.37536819
ATAGGAC853.8176404E-612.326373
GTGTTAG550.002979490412.1363521
GTATAAT550.002979490412.1363521
ACGTGGA951.0544354E-611.9824918
ACAGTCT907.559609E-611.594178
GGTATCA6100.011.56790351
TAGGACT1406.4574124E-1011.5659764
GTATTAG1251.7229468E-811.4428461
TATGGCG2200.011.21283316
Produced by FastQC (version 0.11.5)