##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577221 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.786738528224024 31.0 30.0 33.0 25.0 34.0 2 30.09560636220789 31.0 30.0 34.0 26.0 34.0 3 30.14330039967361 31.0 30.0 34.0 26.0 34.0 4 33.70017722847921 35.0 35.0 37.0 28.0 37.0 5 33.6509205313043 37.0 35.0 37.0 28.0 37.0 6 33.58340566264914 37.0 35.0 37.0 28.0 37.0 7 33.56847030859931 37.0 35.0 37.0 28.0 37.0 8 33.57384606589157 37.0 35.0 37.0 28.0 37.0 9 35.075137598943904 39.0 35.0 39.0 28.0 39.0 10 34.96849560220436 39.0 35.0 39.0 28.0 39.0 11 34.98575069167615 39.0 35.0 39.0 28.0 39.0 12 34.892597462670274 39.0 35.0 39.0 27.0 39.0 13 34.89300805064265 39.0 35.0 39.0 27.0 39.0 14 35.98025712855215 40.0 36.0 41.0 27.0 41.0 15 35.94438525278879 40.0 36.0 41.0 27.0 41.0 16 35.92636789028812 40.0 36.0 41.0 27.0 41.0 17 35.91437248471556 40.0 36.0 41.0 27.0 41.0 18 35.8888692545836 40.0 36.0 41.0 27.0 41.0 19 35.98655454323387 40.0 36.0 41.0 27.0 41.0 20 35.93836676073809 40.0 36.0 41.0 27.0 41.0 21 35.895393618735284 40.0 36.0 41.0 27.0 41.0 22 35.79922074907184 40.0 36.0 41.0 27.0 41.0 23 35.73347816520882 40.0 36.0 41.0 27.0 41.0 24 35.6582418172589 40.0 36.0 41.0 27.0 41.0 25 35.55607124480918 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03633012485883569 1101 2 0.03512181423645799 1101 3 0.4380948716900761 1101 4 0.19295373775212 1101 5 0.0865683527118577 1101 6 0.23222782086775595 1101 7 0.13035894046963392 1101 8 0.13330832250051117 1101 9 0.05548267843681032 1101 10 0.15497949282092094 1101 11 0.43801542540992955 1101 12 0.23510836941423463 1101 13 0.30142448749185746 1101 14 0.3138509611230873 1101 15 0.4252136761425902 1101 16 0.6278974165218116 1101 17 0.5379023906236569 1101 18 0.2926363639177083 1101 19 0.5107968509654626 1101 20 0.26166299495716316 1101 21 0.29273505401984323 1101 22 0.11399585560470626 1101 23 0.112714149743816 1101 24 0.24192383567869058 1101 25 0.26112443604338154 1102 1 -0.5686073283030915 1102 2 -0.48954527517381763 1102 3 -0.3803809454540996 1102 4 -0.5788396559182516 1102 5 -0.778313183000364 1102 6 -0.6657113040529836 1102 7 -0.6804873153671096 1102 8 -0.5722055992150601 1102 9 -0.5513657679804425 1102 10 -0.5034773008609861 1102 11 -0.6902372984039857 1102 12 -0.8907923627008287 1102 13 -0.5894528976467868 1102 14 -0.6759513101630148 1102 15 -0.8593571430192242 1102 16 -0.8696237686975365 1102 17 -0.7423852671846092 1102 18 -0.590960223388457 1102 19 -0.6538598620108473 1102 20 -0.42364570395331214 1102 21 -0.429551220263896 1102 22 -0.47226113407650416 1102 23 -0.39413717901619805 1102 24 -0.5251453981945957 1102 25 -0.3999293484726678 1103 1 -0.6300813694940253 1103 2 -0.7110919215732068 1103 3 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0.5451433619873356 2214 10 0.2878907296047899 2214 11 0.6314012134600873 2214 12 0.476613164103199 2214 13 0.6265904574943164 2214 14 0.6017412979807233 2214 15 0.5256931450385807 2214 16 0.43892520112962785 2214 17 0.42054315777388496 2214 18 0.44450506320465166 2214 19 0.45944450279727533 2214 20 0.3453856406419433 2214 21 0.38479308205033647 2214 22 0.3764743409747595 2214 23 0.41149703639245416 2214 24 0.26769221531970544 2214 25 0.6478345417724256 2215 1 0.4780948376690226 2215 2 0.5199726545478498 2215 3 0.38882212086272006 2215 4 0.56158839367788 2215 5 0.4531525781767698 2215 6 0.5270010486763539 2215 7 0.27659196641505446 2215 8 0.18263053804892593 2215 9 0.340464246939554 2215 10 0.4999311469447463 2215 11 0.3692111761548489 2215 12 0.44513910820517566 2215 13 0.5459203595431106 2215 14 0.6125448569409642 2215 15 0.6234977214145871 2215 16 0.5299749348060914 2215 17 0.6086334085369529 2215 18 0.4127826381642521 2215 19 0.3384228976742918 2215 20 0.4185238695405431 2215 21 0.47676148231366255 2215 22 0.29053662277897985 2215 23 0.5231983151444197 2215 24 0.4056438182482651 2215 25 0.38694619816518383 2216 1 -0.31752852082399485 2216 2 0.001874056107805444 2216 3 -2.0721454951290355 2216 4 -1.361762252074378 2216 5 -0.532416207929117 2216 6 -0.413494413958567 2216 7 -0.1861718009300617 2216 8 -0.1626819816297882 2216 9 -0.14144974899343055 2216 10 -0.334632075354655 2216 11 -0.2896440728407512 2216 12 0.042295492535068036 2216 13 0.03375758599090517 2216 14 -0.08257637358740766 2216 15 -0.22663892753276826 2216 16 -0.011689673164987369 2216 17 -0.2611339927691816 2216 18 -0.27870947533623536 2216 19 -0.23067806753908116 2216 20 -0.07073458346355466 2216 21 -0.023222532520570383 2216 22 -0.1818153376320666 2216 23 -0.2094299270589346 2216 24 -0.26275215271492414 2216 25 -0.14666091746181564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15508.0 3 26.0 4 255.0 5 1616.0 6 2503.0 7 1828.0 8 1388.0 9 1177.0 10 978.0 11 1001.0 12 930.0 13 819.0 14 792.0 15 759.0 16 775.0 17 866.0 18 937.0 19 1150.0 20 1460.0 21 1942.0 22 2412.0 23 2853.0 24 3325.0 25 3955.0 26 4748.0 27 5121.0 28 5854.0 29 7096.0 30 9138.0 31 11747.0 32 15323.0 33 20021.0 34 27150.0 35 37933.0 36 56125.0 37 97149.0 38 230561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89748526508962 20.625058540697495 11.61915763839021 26.858298555822675 2 13.440053562477125 21.916731856317963 41.49253368902889 23.15068089217603 3 17.491184752003466 26.907515702837344 29.66878925709335 25.932510288065842 4 11.006009829857195 18.904890847699583 39.585184877196085 30.503914445247144 5 11.86394868969532 39.59777821668278 35.70272733257796 12.835545761043948 6 28.81796135492451 39.77574382643615 19.139134440706844 12.267160377932496 7 26.569569474167583 33.49731841929698 22.397740367536844 17.535371738998588 8 24.079458035899922 38.26876429412987 20.68386582576755 16.967911844202646 9 25.97408672871806 16.664645260553474 20.756779796103654 36.604488214624816 10 13.47457891399485 31.51275645445757 34.83743879916424 20.175225832383344 11 32.51523325900785 24.78226444972288 24.423111240473258 18.279391050796008 12 21.77856238273515 27.190546395735417 32.150362340809984 18.88052888071945 13 27.248583293342737 23.849873271669853 26.39916565463022 22.502377780357197 14 19.98870449966304 23.806479667233173 29.3055865950823 26.899229238021487 15 22.20836213632434 31.393441060850975 24.719982952094348 21.67821385073034 16 20.487024855384178 30.683847898284704 28.414865068544177 20.41426217778694 17 20.15432563957306 29.99007312623761 28.476961163921615 21.378640070267714 18 19.722982571636464 29.77876719448391 30.16631440352032 20.331935830359306 19 21.24683829389141 28.738955684141228 29.637919684002632 20.37628633796473 20 21.578539786596725 28.703243320969413 30.029261092448767 19.6889557999851 21 21.999632675267613 27.992397071105056 29.05867839333548 20.94929186029185 22 21.024009867970847 29.083661197357685 29.40901318559096 20.483315749080507 23 20.936868201260868 28.559252002266028 29.206837589069007 21.297042207404097 24 20.40604898297186 28.710320657079354 30.18185409054764 20.701776269401147 25 21.058503929368392 28.48410927386381 29.606612704264784 20.850774092503013 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 341.0 2 351.0 3 361.0 4 361.0 5 361.0 6 663.5 7 966.0 8 966.0 9 966.0 10 1443.0 11 1920.0 12 1920.0 13 1920.0 14 3045.0 15 4170.0 16 4170.0 17 4170.0 18 6939.5 19 9709.0 20 9709.0 21 9709.0 22 14793.5 23 19878.0 24 19878.0 25 19878.0 26 26885.5 27 33893.0 28 33893.0 29 33893.0 30 42216.5 31 50540.0 32 50540.0 33 50540.0 34 56982.0 35 63424.0 36 63424.0 37 63424.0 38 67722.5 39 72021.0 40 72021.0 41 72021.0 42 74038.5 43 76056.0 44 76056.0 45 76056.0 46 76456.5 47 76857.0 48 76857.0 49 76857.0 50 71900.0 51 66943.0 52 66943.0 53 66943.0 54 56540.0 55 46137.0 56 46137.0 57 46137.0 58 37286.5 59 28436.0 60 28436.0 61 28436.0 62 21673.5 63 14911.0 64 14911.0 65 14911.0 66 10777.0 67 6643.0 68 6643.0 69 6643.0 70 4680.5 71 2718.0 72 2718.0 73 2718.0 74 1788.5 75 859.0 76 859.0 77 859.0 78 582.5 79 306.0 80 306.0 81 306.0 82 200.5 83 95.0 84 95.0 85 95.0 86 60.5 87 26.0 88 26.0 89 26.0 90 17.0 91 8.0 92 8.0 93 8.0 94 5.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12109746526893513 2 0.12092422139873635 3 0.016631411539081216 4 0.0 5 0.004677584495366593 6 0.3440623262147427 7 0.023561166347031724 8 0.010567876082124525 9 0.011260851562919574 10 0.003984609014571542 11 0.00519731610596288 12 3.464877403975254E-4 13 3.464877403975254E-4 14 0.0 15 0.004157852884770304 16 3.464877403975254E-4 17 0.0 18 1.732438701987627E-4 19 1.732438701987627E-4 20 6.929754807950508E-4 21 0.012993290264907203 22 0.0 23 0.0 24 0.0 25 0.0050240722357641175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 577221.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.96949532549274 #Duplication Level Percentage of deduplicated Percentage of total 1 94.88364096892509 84.41749651650512 2 4.2034154087895885 7.479514951268189 3 0.4245814758086135 1.1332439888173573 4 0.15224091261309125 0.5417918865231668 5 0.0856112321458996 0.3808394059107144 6 0.04774653280007533 0.2548790956058873 7 0.036911224612836364 0.22987811179549703 8 0.02446823513732226 0.174154122533844 9 0.02086979906055311 0.16710979409656676 >10 0.0988608298600178 1.6815639751245341 >50 0.012679933659570883 0.7978454269373446 >100 0.007607922106793189 1.300984598749554 >500 7.802997031627239E-4 0.4517693316855733 >1k 5.852247773720429E-4 0.9889287944465605 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2641 0.45753706119493226 No Hit GGTATCAACGCAGAGTACTTTTTTT 1678 0.2907032141935238 No Hit TATCAACGCAGAGTACTTTTTTTTT 1379 0.23890329700409374 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 823 0.1425797051735817 No Hit CACACACACACACACACACACACAC 685 0.11867205108615243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.732438701987627E-4 2 0.0 0.0 0.0 0.0 1.732438701987627E-4 3 0.0 0.0 0.0 0.0 1.732438701987627E-4 4 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 5 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 6 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 7 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 8 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 9 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 10 0.0 0.0 0.0 1.732438701987627E-4 1.732438701987627E-4 11 0.0 0.0 0.0 3.464877403975254E-4 1.732438701987627E-4 12 0.0 0.0 0.0 3.464877403975254E-4 1.732438701987627E-4 13 0.0 0.0 0.0 5.197316105962881E-4 1.732438701987627E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGCC 25 0.0060725505 18.975565 19 GACGTGG 80 7.821654E-9 15.41631 7 TACTTCA 50 8.8013236E-5 15.179135 7 CTTAATC 40 0.0051828763 14.287382 3 TAGGACG 205 0.0 13.474279 4 TTAGGAC 165 0.0 13.2771635 3 CTAGGAC 110 2.6266207E-9 12.988529 3 AGGACGT 185 0.0 12.871516 5 GGACGTG 185 0.0 12.871516 6 CACTGAC 60 4.1327055E-4 12.6492815 7 GGCGAGG 115 5.3951226E-9 12.375368 19 ATAGGAC 85 3.8176404E-6 12.32637 3 GTGTTAG 55 0.0029794904 12.136352 1 GTATAAT 55 0.0029794904 12.136352 1 ACGTGGA 95 1.0544354E-6 11.982491 8 ACAGTCT 90 7.559609E-6 11.59417 8 GGTATCA 610 0.0 11.5679035 1 TAGGACT 140 6.4574124E-10 11.565976 4 GTATTAG 125 1.7229468E-8 11.442846 1 TATGGCG 220 0.0 11.212833 16 >>END_MODULE