FastQCFastQC Report
Thu 26 May 2016
SRR1926987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742931
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12080.162599218500776No Hit
GTACATGGGAAGCAGTGGTATCAAC9220.12410304590870486No Hit
GTCCTACAGTGGACATTTCTAAATT7940.10687398964372195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG350.00214225316.3157186
CTAGTAC551.894725E-413.8717613
GTACTAG500.00144703113.3615241
TGTAGGA5250.012.1716432
GTCCTAG1353.4197E-1012.0183011
TAGGACC2000.011.9025094
GTAGGAC4600.011.8173853
AAGACTG1052.6482303E-711.7835745
GGACGTG3500.011.6929326
GACGTGA1800.011.63250357
TAGGACG3850.011.6242684
CTCTTTA751.9840957E-411.4527341
GTGTAGG1501.6189006E-1011.4527341
CTAGGAC751.997882E-411.4442033
AGGACGT3750.011.4210025
TAACCTA600.00579084211.1037535
GTGCTAT700.001433519310.9073661
CCTATAC700.001442462710.8992413
CTACACC700.001461319610.8822934
GCGCCAC803.8336622E-410.66682413
Produced by FastQC (version 0.11.5)