##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 742931 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.105802557707243 31.0 28.0 31.0 16.0 34.0 2 28.13562093922585 31.0 28.0 31.0 16.0 34.0 3 27.927819676389866 31.0 28.0 31.0 16.0 34.0 4 31.462493825133155 35.0 32.0 37.0 18.0 37.0 5 31.74036350616679 35.0 32.0 37.0 19.0 37.0 6 30.859665298661653 35.0 31.0 37.0 11.0 37.0 7 30.93331009205431 35.0 32.0 37.0 13.0 37.0 8 30.720679309384046 35.0 31.0 37.0 11.0 37.0 9 32.938387279572396 37.0 33.0 39.0 17.0 39.0 10 33.130030918079875 37.0 33.0 39.0 17.0 39.0 11 33.29178752804769 38.0 33.0 39.0 17.0 39.0 12 33.17478204570815 38.0 33.0 39.0 17.0 39.0 13 33.27121361203127 38.0 34.0 39.0 17.0 39.0 14 34.281377409207586 39.0 34.0 41.0 17.0 41.0 15 33.85016374333552 39.0 33.0 41.0 11.0 41.0 16 33.93218212727696 39.0 33.0 40.0 16.0 41.0 17 34.160616531010284 39.0 34.0 41.0 17.0 41.0 18 34.2048305966503 39.0 34.0 41.0 17.0 41.0 19 33.60879139516321 39.0 33.0 41.0 10.0 41.0 20 33.872443066718176 39.0 34.0 41.0 10.0 41.0 21 33.86184585109519 39.0 34.0 41.0 10.0 41.0 22 33.87930507678371 39.0 34.0 41.0 2.0 41.0 23 33.86892187834402 39.0 34.0 41.0 2.0 41.0 24 32.57897570568465 39.0 32.0 40.0 2.0 41.0 25 33.14461773704422 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.16242754293581285 1101 2 -0.25467988523023877 1101 3 -0.9330210984725014 1101 4 -0.5346407654361229 1101 5 -0.46468457235647165 1101 6 -2.308332277977545 1101 7 -0.5681082011153116 1101 8 -0.2574413782029268 1101 9 -0.2518962733610479 1101 10 -9.243216343581103 1101 11 -4.149962256461329 1101 12 -1.374806075704388 1101 13 -0.8433281839088522 1101 14 -0.5933928169328198 1101 15 -1.7580099025699454 1101 16 -0.25730516430289185 1101 17 -0.3020221939529648 1101 18 -0.12445383075556293 1101 19 -0.6889244626939188 1101 20 -0.20431850864925138 1101 21 -0.3474438997610676 1101 22 -0.05569846682846702 1101 23 -0.13959476663074355 1101 24 -0.07707222070851572 1101 25 -0.06330065046383027 1102 1 -0.25217480657807556 1102 2 -0.22014232392363908 1102 3 -0.6026308113484511 1102 4 -0.6545349014124326 1102 5 -0.10873079152201015 1102 6 -1.138834555668037 1102 7 -0.3251216103387229 1102 8 0.32764839541143687 1102 9 0.09639603519272555 1102 10 -0.020108078347917058 1102 11 -0.2501987541869326 1102 12 -0.11935229981068574 1102 13 -0.17912860354409332 1102 14 -0.5621787403773908 1102 15 0.16860169108875311 1102 16 -0.03025417236292327 1102 17 -0.2658607694329618 1102 18 -0.34622809071201743 1102 19 0.24839375850062595 1102 20 0.05725522231647062 1102 21 0.042296446816799005 1102 22 -0.12083415657507146 1102 23 -0.2837925024449959 1102 24 -3.7941856967968874 1102 25 -1.9736456143468715 1103 1 0.011926448039496051 1103 2 -0.2162218568041503 1103 3 0.09871573206948625 1103 4 0.03648360291999708 1103 5 -0.20929426291434794 1103 6 0.15380626962178567 1103 7 0.14927193442405695 1103 8 0.1934264455620962 1103 9 0.18306848978305368 1103 10 0.2515293933508431 1103 11 -0.0020823828746046047 1103 12 -0.0026592470214197306 1103 13 0.16584015869140956 1103 14 -0.11794538491376017 1103 15 0.3353432602970017 1103 16 0.3995723601601 1103 17 0.10187836544267981 1103 18 0.014063348267946196 1103 19 -1.338186770243226 1103 20 -0.31606074724586364 1103 21 -0.17668964514309948 1103 22 0.10146467051922059 1103 23 -0.080231227058988 1103 24 1.4138084471862271 1103 25 0.8619194742594303 1104 1 -0.019908137288194183 1104 2 0.07085890734703071 1104 3 -1.383835981045749 1104 4 -0.2093461202392355 1104 5 0.17154559677255676 1104 6 -1.2755400294941062 1104 7 0.15128914549095995 1104 8 0.4949052850348288 1104 9 0.36071345054081405 1104 10 0.4450302387624845 1104 11 0.5920830149735892 1104 12 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1.2698720588845447 1110 7 1.3529942216589532 1110 8 1.4357710697265027 1110 9 1.2355075337227177 1110 10 1.3422926065086926 1110 11 1.107282648144455 1110 12 1.1434942291973016 1110 13 1.08404441960419 1110 14 0.9610257761769603 1110 15 1.455415077036072 1110 16 1.2188827270987517 1110 17 1.0407828395614018 1110 18 1.1974252693657164 1110 19 -1.0764163593809428 1110 20 0.5975171049847816 1110 21 0.6992870144167043 1110 22 1.2207958771366236 1110 23 1.1901379558652962 1110 24 -0.10114642689402586 1110 25 0.5187669117804674 1111 1 0.2065588886090275 1111 2 -0.33472527523230156 1111 3 0.5438985709329387 1111 4 0.4258629543594985 1111 5 0.39794088730831945 1111 6 0.35763553638462753 1111 7 0.46285469801936685 1111 8 0.9717053140310163 1111 9 0.09179645817043536 1111 10 -0.138315679721984 1111 11 -0.3253888496625379 1111 12 -0.19254822651085135 1111 13 -0.3780427366231507 1111 14 -0.3339006630922725 1111 15 -1.2030685818907898 1111 16 -1.0542819959836578 1111 17 -0.7341374408673005 1111 18 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0.44396584169193076 2215 2 -0.08878324548867411 2215 3 0.30557684176419997 2215 4 0.5442705508588972 2215 5 0.6680908085813435 2215 6 1.1703111027742246 2215 7 0.6618730955461345 2215 8 -0.5574079743503475 2215 9 -0.050826222522360354 2215 10 -0.16003229399117913 2215 11 -0.21995812678672877 2215 12 -0.5724307376137077 2215 13 -0.08792401843433595 2215 14 -0.17770019439213058 2215 15 0.02820825982352204 2215 16 0.20387448499893424 2215 17 -0.24617926817012403 2215 18 -0.9903014218022079 2215 19 0.2621621491265316 2215 20 -3.2130683448012434 2215 21 -1.9546611051412022 2215 22 -0.9772825705410852 2215 23 -0.7899987841135001 2215 24 -0.9920044475333647 2215 25 -0.7326398602442552 2216 1 -0.8249963384159997 2216 2 -1.4097814643824513 2216 3 -0.5909566931169579 2216 4 -0.893176041692918 2216 5 -0.9422042397495574 2216 6 -1.8110166540796442 2216 7 -10.210170077354402 2216 8 -11.12035624111779 2216 9 -5.9533256456765 2216 10 -3.8957115277218577 2216 11 -2.7849854611047853 2216 12 -2.3575539888320485 2216 13 -2.2485045827561336 2216 14 -2.4413360129772848 2216 15 -3.4452607670359967 2216 16 -2.4398673278777174 2216 17 -2.73769130683727 2216 18 -2.390287856083262 2216 19 -1.7886475244423004 2216 20 -2.778390006771385 2216 21 -5.440705068971603 2216 22 -3.904675087956985 2216 23 -3.694609372278258 2216 24 -3.893437992770494 2216 25 -3.381857620919533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44591.0 3 1.0 4 341.0 5 1963.0 6 2733.0 7 2119.0 8 1838.0 9 1623.0 10 1480.0 11 1429.0 12 1515.0 13 1276.0 14 1433.0 15 1478.0 16 1544.0 17 1765.0 18 1943.0 19 2344.0 20 3125.0 21 4058.0 22 5016.0 23 5936.0 24 7155.0 25 7944.0 26 9128.0 27 9887.0 28 11020.0 29 13054.0 30 15519.0 31 18869.0 32 24112.0 33 31485.0 34 42812.0 35 59922.0 36 86724.0 37 136272.0 38 179477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76800157582512 18.997866969599258 12.854847348687734 25.379284105887894 2 14.11271190096191 20.424853340810074 42.14807186300306 23.31436289522495 3 18.646567284089578 24.78031184327766 29.95981651203011 26.61330436060266 4 11.762590364123474 17.02408228304192 39.17455648001854 32.03877087281607 5 12.580409770073159 38.07700489257605 36.678298988364986 12.664286348985806 6 30.703821081513354 37.56283876387703 19.442252959850506 12.291087194759106 7 27.370308462117997 31.493353063963426 22.88271526594495 18.253623207973625 8 24.092022924725086 37.24498268494704 21.89108730431965 16.771907086008223 9 25.806855495018116 16.013925855321347 20.736115542595428 37.44310310706511 10 13.864385828605899 29.80794478159997 35.28104048873563 21.046628901058504 11 33.68600457453955 23.522126205057305 24.388569305351187 18.403299915051957 12 22.42526884905142 25.60501133554839 32.14879993968734 19.820919875712846 13 28.15307735180005 22.626660240652438 26.00987595818065 23.21038644936687 14 20.1838444161432 22.79544212320376 28.249520060527573 28.771193400125473 15 22.84960784472105 31.124043843848696 23.65545799913303 22.370890312297227 16 21.444188350853008 29.1695836205411 28.142770980527317 21.24345704807858 17 21.180287701017118 29.19121953189685 28.15067617143588 21.477816595650154 18 20.73670804961646 28.68508840658142 29.691122186053647 20.887081357748468 19 22.88689302154002 27.91639796057122 28.03164426589046 21.165064751998298 20 22.503695486525274 28.163242227361945 28.707400935382164 20.625661350730613 21 22.37911829760653 27.552156464682227 27.889118795713053 22.179606441998185 22 22.064830197010778 28.519040067204987 27.936381431390867 21.479748304393365 23 21.561756184773127 28.268882809723316 28.178144083404238 21.991216922099323 24 22.311239154286792 27.89870827471544 28.521078883421623 21.26897368757615 25 21.944836350712432 27.97425445067904 28.414072007840517 21.666837190768003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 236.0 1 236.0 2 320.5 3 405.0 4 405.0 5 405.0 6 640.5 7 876.0 8 876.0 9 876.0 10 1264.5 11 1653.0 12 1653.0 13 1653.0 14 2958.5 15 4264.0 16 4264.0 17 4264.0 18 7252.0 19 10240.0 20 10240.0 21 10240.0 22 15983.5 23 21727.0 24 21727.0 25 21727.0 26 30061.0 27 38395.0 28 38395.0 29 38395.0 30 48916.5 31 59438.0 32 59438.0 33 59438.0 34 67482.5 35 75527.0 36 75527.0 37 75527.0 38 81611.0 39 87695.0 40 87695.0 41 87695.0 42 91471.5 43 95248.0 44 95248.0 45 95248.0 46 96724.0 47 98200.0 48 98200.0 49 98200.0 50 93016.5 51 87833.0 52 87833.0 53 87833.0 54 76744.5 55 65656.0 56 65656.0 57 65656.0 58 54989.0 59 44322.0 60 44322.0 61 44322.0 62 35583.0 63 26844.0 64 26844.0 65 26844.0 66 20516.5 67 14189.0 68 14189.0 69 14189.0 70 10303.0 71 6417.0 72 6417.0 73 6417.0 74 4435.0 75 2453.0 76 2453.0 77 2453.0 78 1681.0 79 909.0 80 909.0 81 909.0 82 591.5 83 274.0 84 274.0 85 274.0 86 185.5 87 97.0 88 97.0 89 97.0 90 59.5 91 22.0 92 22.0 93 22.0 94 13.5 95 5.0 96 5.0 97 5.0 98 5.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23313066758554965 2 0.14469715222544222 3 0.1459085702440738 4 0.06972383707235262 5 0.02328614635814093 6 0.02113251432501807 7 0.30810398273863926 8 0.30231609664962156 9 0.01884428028982503 10 0.03768856057965006 11 0.01669064825670217 12 0.018171270279474138 13 0.01574843424221092 14 0.017363658267053064 15 0.015210026233930203 16 0.019786494304316282 17 0.019651892302246104 18 0.015210026233930203 19 0.02342074836021111 20 0.017902066275333778 21 0.0158830362442811 22 0.017363658267053064 23 0.01870967828775485 24 0.01695985226084253 25 0.01655604625463199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 742931.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.1450210326287 #Duplication Level Percentage of deduplicated Percentage of total 1 93.71324090895979 82.60355590956063 2 4.951278354526394 8.728610693962567 3 0.7366820543692113 1.948045655302028 4 0.21946479447442066 0.7737891569947739 5 0.10008217431698632 0.4410872680080985 6 0.06345463204489309 0.33559259257288904 7 0.04407275682854737 0.27193558543327667 8 0.027864520721945583 0.19648950120800096 9 0.01917277557966904 0.15209862360514181 >10 0.1049996800849278 1.6629957014481698 >50 0.010378375820922324 0.6293155545490412 >100 0.007935517370076427 1.340976851016304 >500 0.0012208488019459394 0.7530131739179778 >1k 1.5260610024324243E-4 0.16249373242102846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1208 0.162599218500776 No Hit GTACATGGGAAGCAGTGGTATCAAC 922 0.12410304590870486 No Hit GTCCTACAGTGGACATTTCTAAATT 794 0.10687398964372195 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.346020020701788E-4 0.0 10 0.0 0.0 0.0 1.346020020701788E-4 0.0 11 0.0 0.0 0.0 1.346020020701788E-4 0.0 12 0.0 0.0 0.0 1.346020020701788E-4 0.0 13 0.0 0.0 0.0 2.692040041403576E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACCGG 35 0.002142253 16.315718 6 CTAGTAC 55 1.894725E-4 13.871761 3 GTACTAG 50 0.001447031 13.361524 1 TGTAGGA 525 0.0 12.171643 2 GTCCTAG 135 3.4197E-10 12.018301 1 TAGGACC 200 0.0 11.902509 4 GTAGGAC 460 0.0 11.817385 3 AAGACTG 105 2.6482303E-7 11.783574 5 GGACGTG 350 0.0 11.692932 6 GACGTGA 180 0.0 11.6325035 7 TAGGACG 385 0.0 11.624268 4 CTCTTTA 75 1.9840957E-4 11.452734 1 GTGTAGG 150 1.6189006E-10 11.452734 1 CTAGGAC 75 1.997882E-4 11.444203 3 AGGACGT 375 0.0 11.421002 5 TAACCTA 60 0.005790842 11.103753 5 GTGCTAT 70 0.0014335193 10.907366 1 CCTATAC 70 0.0014424627 10.899241 3 CTACACC 70 0.0014613196 10.882293 4 GCGCCAC 80 3.8336622E-4 10.666824 13 >>END_MODULE