FastQCFastQC Report
Thu 26 May 2016
SRR1926987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18310.24645626579049734No Hit
GGTATCAACGCAGAGTACTTTTTTT11590.15600372039933721No Hit
GTACATGGGAAGCAGTGGTATCAAC10630.14308192820060006No Hit
TATCAACGCAGAGTACTTTTTTTTT8890.11966117984038895No Hit
CCCATGTACTCTGCGTTGATACCAC8810.11858436382382752No Hit
GTCCTACAGTGGACATTTCTAAATT8020.10795080566028338No Hit
CTGTAGGACGTGGAATATGGCAAGA7680.10337433758989731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTC400.00519302314.2839465
GTATAAC551.9002285E-413.8670251
TAGGACC2650.013.2957484
GTACCAT655.3247604E-513.1851816
ATCTAGG603.9676396E-412.7114381
GTGTAAC603.9676396E-412.7114381
TTAATCC1001.3945464E-712.3794194
GTCCTAC4750.012.0424161
GGACCTG4150.011.9319726
TGGCGAG5550.011.79591618
GCCCTAG657.7809225E-411.7336361
CTTAAAC657.8563206E-411.72016053
ATAGGAC1156.821392E-811.5927673
TATGGCG5650.011.58713816
GTAGGAC5750.011.4271563
TCCTACA5800.011.3409042
TGTAGGA6100.011.2519882
ATGGCGA6000.011.22748917
AGGACCT4500.011.2155435
CCTACAC1701.0913936E-1111.20309453
Produced by FastQC (version 0.11.5)