##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 742931 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.863875649286406 31.0 30.0 33.0 26.0 34.0 2 30.170532660502793 31.0 30.0 34.0 26.0 34.0 3 30.22595368883517 31.0 30.0 34.0 26.0 34.0 4 33.80622022771967 35.0 35.0 37.0 28.0 37.0 5 33.7511182061322 37.0 35.0 37.0 28.0 37.0 6 33.71218726907344 37.0 35.0 37.0 29.0 37.0 7 33.7048568440407 37.0 35.0 37.0 29.0 37.0 8 33.72261757821386 37.0 35.0 37.0 29.0 37.0 9 35.25528750314632 39.0 35.0 39.0 29.0 39.0 10 35.14080446232557 39.0 35.0 39.0 29.0 39.0 11 35.18246782002635 39.0 35.0 39.0 29.0 39.0 12 35.0695515465097 39.0 35.0 39.0 29.0 39.0 13 35.08776588942984 39.0 35.0 39.0 29.0 39.0 14 36.17961829564253 40.0 36.0 41.0 29.0 41.0 15 36.160014860061025 40.0 36.0 41.0 29.0 41.0 16 36.144473712902006 40.0 36.0 41.0 28.0 41.0 17 36.1090572341173 40.0 36.0 41.0 28.0 41.0 18 36.087605713047374 40.0 36.0 41.0 28.0 41.0 19 36.16036078720635 40.0 37.0 41.0 28.0 41.0 20 36.12171386037196 40.0 36.0 41.0 28.0 41.0 21 36.0698893975349 40.0 36.0 41.0 28.0 41.0 22 35.98163624885756 40.0 36.0 41.0 27.0 41.0 23 35.91916611367677 40.0 36.0 41.0 27.0 41.0 24 35.84104311167524 40.0 36.0 41.0 27.0 41.0 25 35.73107327598391 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14146437437631576 1101 2 0.0734922205692321 1101 3 0.4692848673144745 1101 4 0.3130079694544037 1101 5 0.3086357629925516 1101 6 0.2722673919023322 1101 7 0.5257195215808395 1101 8 0.4224225164736666 1101 9 0.3649238929564689 1101 10 0.49422131265828284 1101 11 0.3628361183029156 1101 12 0.49895017533552277 1101 13 0.35181841527503366 1101 14 0.4752507296592796 1101 15 0.35045910379042766 1101 16 0.4163873707038235 1101 17 0.40699555587719516 1101 18 0.43508104407410286 1101 19 0.4230064320537963 1101 20 0.40774282703440434 1101 21 0.3868960249187978 1101 22 0.25293679602925323 1101 23 0.47735408104413324 1101 24 0.513607115944616 1101 25 0.5521751878101071 1102 1 -0.5381503780751231 1102 2 -0.5040101921687068 1102 3 -0.4227282289941172 1102 4 -0.4584489289997933 1102 5 -0.4214801472437202 1102 6 -0.38081712474308915 1102 7 -0.307709333117991 1102 8 -0.4553414271753198 1102 9 -0.1633043174672153 1102 10 -0.39536916419096 1102 11 -0.4354869876566312 1102 12 -0.350210060108175 1102 13 -0.12861288015938044 1102 14 -0.7130251755703512 1102 15 -0.48371909955150016 1102 16 -0.4547559541643409 1102 17 -0.5072032010275223 1102 18 -0.5429424541818051 1102 19 -0.6138071728237264 1102 20 -0.6440775137462964 1102 21 -0.6334566136118838 1102 22 -0.6351368674127471 1102 23 -0.43772742503135476 1102 24 -0.5568020119453152 1102 25 -0.5752338445584613 1103 1 -0.02767253390645763 1103 2 -0.26803994890568106 1103 3 0.11046551323899934 1103 4 0.16462616079813586 1103 5 0.00629677936881734 1103 6 0.014893002420997448 1103 7 -0.1130393298835628 1103 8 0.04342097855813165 1103 9 -0.023371048822440343 1103 10 0.002196503207713363 1103 11 -0.04371945790840215 1103 12 -0.28348040721336076 1103 13 -0.08492086579170888 1103 14 -0.0212644551440917 1103 15 -0.08755445014646313 1103 16 -0.040452973383658275 1103 17 0.06208335226394013 1103 18 -0.027922792264639895 1103 19 -0.19418611159089494 1103 20 -0.38809297090715233 1103 21 3.891211990705301E-4 1103 22 -0.21385943861272239 1103 23 -0.11742883626333622 1103 24 0.05669917117437251 1103 25 -0.015608016800257474 1104 1 -0.17164487506580883 1104 2 -0.2246076617517332 1104 3 -0.049599932557917725 1104 4 -0.21947421185595317 1104 5 -0.2667089324173162 1104 6 -0.3046414743911683 1104 7 -0.427641818169775 1104 8 -0.31813508359013554 1104 9 -0.41917006797669387 1104 10 -0.4169114823572002 1104 11 -0.4146398209727451 1104 12 -0.2282224515112503 1104 13 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1108 22 0.6968063786806269 1108 23 0.6313934646244732 1108 24 0.5629372268620827 1108 25 0.4245264852277586 1109 1 0.2729148480820811 1109 2 0.0935440007427637 1109 3 0.06400684796746603 1109 4 0.34644268517726573 1109 5 0.3399876151000285 1109 6 0.2712428315966804 1109 7 0.27780353552589787 1109 8 0.2103032564270677 1109 9 0.20574280498999542 1109 10 0.19081112196430183 1109 11 0.14610480310798124 1109 12 0.23247323637814787 1109 13 0.33670921831251377 1109 14 0.24953687340141073 1109 15 0.18747349211412256 1109 16 0.24681803879996522 1109 17 0.26754338888613205 1109 18 0.15205469538923921 1109 19 0.12887556748133733 1109 20 0.22163252115407062 1109 21 0.15675876758084684 1109 22 0.2738927353347904 1109 23 0.06380902569793534 1109 24 0.13145839212037203 1109 25 0.18728370991254195 1110 1 0.5176457521101163 1110 2 0.4085971681652225 1110 3 0.5775420269210514 1110 4 0.631144812310616 1110 5 0.46086948580186515 1110 6 0.27968676975400086 1110 7 0.4311968958903094 1110 8 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0.01966049992145713 2214 8 0.20742474076290307 2214 9 0.17215312185027898 2214 10 -0.056450596012979304 2214 11 0.3004939128479194 2214 12 0.2710327455739545 2214 13 0.16840987978172706 2214 14 0.24662990453462896 2214 15 0.21122581917962435 2214 16 0.1561281976772264 2214 17 0.04291551612885769 2214 18 0.14164963017720567 2214 19 0.34188775900120305 2214 20 0.4094615959914165 2214 21 0.4574522127759124 2214 22 0.3722771353922383 2214 23 0.21286586977709732 2214 24 0.27071039807115227 2214 25 0.20839395387843496 2215 1 0.22392819744019477 2215 2 0.028215905873931746 2215 3 0.009247573392990205 2215 4 -0.07733084709079208 2215 5 -0.130461402753987 2215 6 -0.15258028230157805 2215 7 -0.32457625050622596 2215 8 -0.23142477577057718 2215 9 -0.27400442124834257 2215 10 -0.13766188943220214 2215 11 -0.4947338007526412 2215 12 -0.07023385452216502 2215 13 -0.28118036273593106 2215 14 0.010195105902234047 2215 15 -0.28017251091233675 2215 16 -0.25564881257480465 2215 17 -0.18703487968367227 2215 18 -0.2059015365919734 2215 19 -0.36522653233375735 2215 20 -0.42292007735305504 2215 21 -0.47968095404804956 2215 22 -0.37678220286997544 2215 23 -0.3813335280997876 2215 24 -0.18595130485653755 2215 25 -0.22691329575364705 2216 1 -0.36987239012500694 2216 2 -0.21480668165372307 2216 3 -2.1779666153800434 2216 4 -1.5147114278852456 2216 5 -0.6272834514076777 2216 6 -0.2600062416371216 2216 7 -0.41435587419076114 2216 8 -0.3231048113321435 2216 9 -0.3104822761205881 2216 10 -0.3832619635591712 2216 11 -0.5178809143128476 2216 12 -0.08735451856411203 2216 13 -0.347389549755718 2216 14 -0.38978648347919886 2216 15 -0.39867572762288006 2216 16 -0.4627616097109808 2216 17 -0.6569095966355576 2216 18 -0.40054113964934857 2216 19 -0.2720924212729088 2216 20 -0.4421337380320338 2216 21 -0.14274240978292596 2216 22 -0.24580215840590824 2216 23 -0.3760386584245552 2216 24 -0.41092966935165975 2216 25 -0.32631973498860845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18221.0 3 21.0 4 300.0 5 2029.0 6 2722.0 7 2100.0 8 1540.0 9 1328.0 10 1189.0 11 1099.0 12 1089.0 13 911.0 14 959.0 15 978.0 16 973.0 17 1006.0 18 1203.0 19 1362.0 20 1726.0 21 2301.0 22 2987.0 23 3598.0 24 4241.0 25 5046.0 26 6052.0 27 6699.0 28 7832.0 29 9139.0 30 11977.0 31 15363.0 32 19946.0 33 25830.0 34 35489.0 35 49361.0 36 72597.0 37 124778.0 38 298939.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29188311469556 19.886806945202434 10.729084551169326 28.092225388932686 2 13.981891123038755 21.043921910016213 40.384763100905985 24.589423866039052 3 18.163215302141364 25.057014154588465 29.34714417647867 27.432626366791514 4 11.792750605372504 17.711739044406546 38.30073048506524 32.194779865155716 5 12.792034373771694 38.7096643926708 35.47487387130296 13.023427362254541 6 31.095606795428115 38.54996832959906 17.882364381130653 12.472060493842168 7 27.860750103332073 32.108876325982735 21.87637579754184 18.153997773143356 8 24.76576508661307 38.08148144159473 20.010123256026837 17.142630215765355 9 25.975879156160858 16.19628912165828 20.213423294020405 37.61440842816046 10 14.096377271020188 30.277307768413486 34.32970831600244 21.296606644563887 11 33.685563714437635 23.729917820389428 24.038310101832717 18.54620836334022 12 22.480899795941443 25.756335775934012 31.766641002309793 19.996123425814755 13 28.332720892575196 22.52462886763069 25.875958537087662 23.26669170270645 14 20.40754794186809 22.900915428216077 27.872305772676064 28.81923085723977 15 22.624422207684873 31.142366411186224 23.639882947420563 22.593328433708344 16 21.53355165836843 29.48047525402831 27.610444605069933 21.375528482533323 17 21.026072442895025 29.409769426460098 27.96710322641433 21.597054904230546 18 20.85983874658447 28.725721131196746 29.45216911418303 20.962271008035753 19 22.428223385783316 27.933048874052734 28.242903099888277 21.395824640275666 20 22.580714633758948 28.096068787470085 28.536893311043094 20.786323267727877 21 22.259840611706423 27.505250121156642 27.85081578805665 22.384093479080285 22 22.112282298086903 28.61275138606412 27.805812383653393 21.469153932195585 23 21.52833114379859 28.446094372536773 27.972697219649817 22.052877264014818 24 21.85451946412251 27.94566386380431 28.75677552827921 21.443041143793973 25 21.921726676035558 27.91929830229215 28.323129856074438 21.83584516559786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 315.0 1 315.0 2 339.0 3 363.0 4 363.0 5 363.0 6 572.5 7 782.0 8 782.0 9 782.0 10 1198.0 11 1614.0 12 1614.0 13 1614.0 14 2773.0 15 3932.0 16 3932.0 17 3932.0 18 6926.5 19 9921.0 20 9921.0 21 9921.0 22 15166.0 23 20411.0 24 20411.0 25 20411.0 26 28615.5 27 36820.0 28 36820.0 29 36820.0 30 47108.0 31 57396.0 32 57396.0 33 57396.0 34 65435.5 35 73475.0 36 73475.0 37 73475.0 38 80022.5 39 86570.0 40 86570.0 41 86570.0 42 90696.5 43 94823.0 44 94823.0 45 94823.0 46 97348.5 47 99874.0 48 99874.0 49 99874.0 50 95201.0 51 90528.0 52 90528.0 53 90528.0 54 78702.5 55 66877.0 56 66877.0 57 66877.0 58 56365.5 59 45854.0 60 45854.0 61 45854.0 62 36732.5 63 27611.0 64 27611.0 65 27611.0 66 21241.0 67 14871.0 68 14871.0 69 14871.0 70 10813.5 71 6756.0 72 6756.0 73 6756.0 74 4713.0 75 2670.0 76 2670.0 77 2670.0 78 1848.0 79 1026.0 80 1026.0 81 1026.0 82 675.0 83 324.0 84 324.0 85 324.0 86 209.5 87 95.0 88 95.0 89 95.0 90 57.0 91 19.0 92 19.0 93 19.0 94 11.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11252727373066947 2 0.11629612978863449 3 0.017902066275333778 4 0.0 5 0.005249478080736973 6 0.33542818915888556 7 0.024228360372632183 8 0.011710374180105555 9 0.011844976182175733 10 0.005114876078666794 11 0.006191692095228224 12 9.422140144912517E-4 13 2.692040041403576E-4 14 0.0 15 0.0022882340351930396 16 2.692040041403576E-4 17 1.346020020701788E-4 18 1.346020020701788E-4 19 1.346020020701788E-4 20 6.73010010350894E-4 21 0.01224878218838627 22 0.0 23 4.0380600621053636E-4 24 0.0 25 0.0063262940972984025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 742931.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.28790784217091 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79174513990156 78.28725900163963 2 6.48892856529634 11.06854282942849 3 0.9511357440720623 2.433611330574381 4 0.2834998476906005 0.967164355324217 5 0.1410381387082024 0.6014423888188242 6 0.08142781299965961 0.4166884686542697 7 0.051394532177097135 0.30683324867382233 8 0.035698715757747114 0.2435735023704472 9 0.025141963106617732 0.19298748891676268 >10 0.1282052810369952 1.9611991755352436 >50 0.010418956993557781 0.6179801786177566 >100 0.009313233823255093 1.408345321554638 >500 0.0015785141817692974 0.9487247285230255 >1k 4.735542545307892E-4 0.5456479813685005 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1831 0.24645626579049734 No Hit GGTATCAACGCAGAGTACTTTTTTT 1159 0.15600372039933721 No Hit GTACATGGGAAGCAGTGGTATCAAC 1063 0.14308192820060006 No Hit TATCAACGCAGAGTACTTTTTTTTT 889 0.11966117984038895 No Hit CCCATGTACTCTGCGTTGATACCAC 881 0.11858436382382752 No Hit GTCCTACAGTGGACATTTCTAAATT 802 0.10795080566028338 No Hit CTGTAGGACGTGGAATATGGCAAGA 768 0.10337433758989731 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.346020020701788E-4 0.0 8 0.0 0.0 0.0 1.346020020701788E-4 0.0 9 0.0 0.0 0.0 1.346020020701788E-4 0.0 10 0.0 0.0 0.0 1.346020020701788E-4 0.0 11 0.0 0.0 0.0 1.346020020701788E-4 0.0 12 0.0 0.0 0.0 1.346020020701788E-4 0.0 13 0.0 0.0 0.0 1.346020020701788E-4 1.346020020701788E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGGTC 40 0.005193023 14.283946 5 GTATAAC 55 1.9002285E-4 13.867025 1 TAGGACC 265 0.0 13.295748 4 GTACCAT 65 5.3247604E-5 13.185181 6 ATCTAGG 60 3.9676396E-4 12.711438 1 GTGTAAC 60 3.9676396E-4 12.711438 1 TTAATCC 100 1.3945464E-7 12.379419 4 GTCCTAC 475 0.0 12.042416 1 GGACCTG 415 0.0 11.931972 6 TGGCGAG 555 0.0 11.795916 18 GCCCTAG 65 7.7809225E-4 11.733636 1 CTTAAAC 65 7.8563206E-4 11.7201605 3 ATAGGAC 115 6.821392E-8 11.592767 3 TATGGCG 565 0.0 11.587138 16 GTAGGAC 575 0.0 11.427156 3 TCCTACA 580 0.0 11.340904 2 TGTAGGA 610 0.0 11.251988 2 ATGGCGA 600 0.0 11.227489 17 AGGACCT 450 0.0 11.215543 5 CCTACAC 170 1.0913936E-11 11.2030945 3 >>END_MODULE