FastQCFastQC Report
Thu 26 May 2016
SRR1926986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412534
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10750.260584582119292No Hit
GGTATCAACGCAGAGTACTTTTTTT6640.16095643025786965No Hit
TATCAACGCAGAGTACTTTTTTTTT5330.12920147187868153No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG5280.12798945056649877No Hit
GTCCTACAGTGGACATTTCTAAATT4670.11320279055786918No Hit
CACACACACACACACACACACACAC4520.1095667266213209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATCC453.5082507E-516.9003358
TAGGACC1500.016.497874
TAGCACT456.6512084E-414.8057814
GGTCTAG400.005146037314.3013591
CCTAAGC400.00517524114.289173
AGGACCT2200.014.2717945
GTGTTAG759.240284E-713.983551
ACTGTAC551.9434457E-413.8275488
GTTACAC500.001465637913.336563
CCAGTAC500.001465637913.336563
TGGCGAG2000.012.332918
TGTTAGG550.002999532712.1241452
TAATACT550.003018740612.1138214
TATAACT550.003018740612.1138214
GTAGCCC959.998803E-712.0329873
GGCGAGA1501.4551915E-1112.0166719
GGACCTG2550.011.5652786
GTAGGAC3100.011.3698783
TATGGCG2100.011.29386416
CTACACT1354.5420165E-911.2805964
Produced by FastQC (version 0.11.5)